Mite and cockroach allergens activate protease-activated receptor 2 and delay epidermal permeability barrier recovery

Se Kyoo Jeong, Hyun Jeong Kim, Jong Kyung Youm, Sung Ku Ahn, Eung Ho Choi, Myung Hyun Sohn, Kyu Earn Kim, Jeong Hee Hong, DongMin Shin, Seung Hun Lee

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Protease-activated receptor-2 (PAR-2) is known to be involved in epidermal permeability barrier function homeostasis. PAR-2 activation occurs after barrier disruption and further activation of PAR-2 by activating peptide significantly delays barrier recovery rate. Cockroach and house dust mite allergens, both known to be associated with the development of asthma, allergic rhinitis, and atopic dermatitis, have protease activity, which can activate PAR-2. In this study, we investigated the effects of both allergens on the epidermal barrier function as well as on the epidermal calcium gradient. Both allergens, when topically applied on the barrier-disrupted site, increased protease activities in the epidermis and delayed barrier recovery and lamellar body secretion in murine skin. The topical application of PAR-2-specific antagonist or protease inhibitors normalized the barrier recovery. Cockroach allergens induced intracellular calcium oscillations in cultured human keratinocytes through PAR-2-involved pathway, which was confirmed by desensitization protocol. Abnormal calcium ion distribution after barrier disruption was also observed in allergens-applied skin. These results suggest that allergens with protease activity can influence the epidermal permeability barrier homeostasis through PAR-2 activation and consequent modulation of the calcium ions in skin.

Original languageEnglish
Pages (from-to)1930-1939
Number of pages10
JournalJournal of Investigative Dermatology
Volume128
Issue number8
DOIs
Publication statusPublished - 2008 Jan 1

Fingerprint

PAR-2 Receptor
Cockroaches
Mites
Allergens
Permeability
Recovery
Calcium
Skin
Peptide Hydrolases
Chemical activation
Homeostasis
Ions
Dermatophagoides Antigens
Calcium Signaling
Atopic Dermatitis
Protease Inhibitors
Keratinocytes
Epidermis
Asthma
Modulation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Jeong, Se Kyoo ; Kim, Hyun Jeong ; Youm, Jong Kyung ; Ahn, Sung Ku ; Choi, Eung Ho ; Sohn, Myung Hyun ; Kim, Kyu Earn ; Hong, Jeong Hee ; Shin, DongMin ; Lee, Seung Hun. / Mite and cockroach allergens activate protease-activated receptor 2 and delay epidermal permeability barrier recovery. In: Journal of Investigative Dermatology. 2008 ; Vol. 128, No. 8. pp. 1930-1939.
@article{bafdf9a5524c4d50a82ec8a56c68886b,
title = "Mite and cockroach allergens activate protease-activated receptor 2 and delay epidermal permeability barrier recovery",
abstract = "Protease-activated receptor-2 (PAR-2) is known to be involved in epidermal permeability barrier function homeostasis. PAR-2 activation occurs after barrier disruption and further activation of PAR-2 by activating peptide significantly delays barrier recovery rate. Cockroach and house dust mite allergens, both known to be associated with the development of asthma, allergic rhinitis, and atopic dermatitis, have protease activity, which can activate PAR-2. In this study, we investigated the effects of both allergens on the epidermal barrier function as well as on the epidermal calcium gradient. Both allergens, when topically applied on the barrier-disrupted site, increased protease activities in the epidermis and delayed barrier recovery and lamellar body secretion in murine skin. The topical application of PAR-2-specific antagonist or protease inhibitors normalized the barrier recovery. Cockroach allergens induced intracellular calcium oscillations in cultured human keratinocytes through PAR-2-involved pathway, which was confirmed by desensitization protocol. Abnormal calcium ion distribution after barrier disruption was also observed in allergens-applied skin. These results suggest that allergens with protease activity can influence the epidermal permeability barrier homeostasis through PAR-2 activation and consequent modulation of the calcium ions in skin.",
author = "Jeong, {Se Kyoo} and Kim, {Hyun Jeong} and Youm, {Jong Kyung} and Ahn, {Sung Ku} and Choi, {Eung Ho} and Sohn, {Myung Hyun} and Kim, {Kyu Earn} and Hong, {Jeong Hee} and DongMin Shin and Lee, {Seung Hun}",
year = "2008",
month = "1",
day = "1",
doi = "10.1038/jid.2008.13",
language = "English",
volume = "128",
pages = "1930--1939",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "8",

}

Mite and cockroach allergens activate protease-activated receptor 2 and delay epidermal permeability barrier recovery. / Jeong, Se Kyoo; Kim, Hyun Jeong; Youm, Jong Kyung; Ahn, Sung Ku; Choi, Eung Ho; Sohn, Myung Hyun; Kim, Kyu Earn; Hong, Jeong Hee; Shin, DongMin; Lee, Seung Hun.

In: Journal of Investigative Dermatology, Vol. 128, No. 8, 01.01.2008, p. 1930-1939.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mite and cockroach allergens activate protease-activated receptor 2 and delay epidermal permeability barrier recovery

AU - Jeong, Se Kyoo

AU - Kim, Hyun Jeong

AU - Youm, Jong Kyung

AU - Ahn, Sung Ku

AU - Choi, Eung Ho

AU - Sohn, Myung Hyun

AU - Kim, Kyu Earn

AU - Hong, Jeong Hee

AU - Shin, DongMin

AU - Lee, Seung Hun

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Protease-activated receptor-2 (PAR-2) is known to be involved in epidermal permeability barrier function homeostasis. PAR-2 activation occurs after barrier disruption and further activation of PAR-2 by activating peptide significantly delays barrier recovery rate. Cockroach and house dust mite allergens, both known to be associated with the development of asthma, allergic rhinitis, and atopic dermatitis, have protease activity, which can activate PAR-2. In this study, we investigated the effects of both allergens on the epidermal barrier function as well as on the epidermal calcium gradient. Both allergens, when topically applied on the barrier-disrupted site, increased protease activities in the epidermis and delayed barrier recovery and lamellar body secretion in murine skin. The topical application of PAR-2-specific antagonist or protease inhibitors normalized the barrier recovery. Cockroach allergens induced intracellular calcium oscillations in cultured human keratinocytes through PAR-2-involved pathway, which was confirmed by desensitization protocol. Abnormal calcium ion distribution after barrier disruption was also observed in allergens-applied skin. These results suggest that allergens with protease activity can influence the epidermal permeability barrier homeostasis through PAR-2 activation and consequent modulation of the calcium ions in skin.

AB - Protease-activated receptor-2 (PAR-2) is known to be involved in epidermal permeability barrier function homeostasis. PAR-2 activation occurs after barrier disruption and further activation of PAR-2 by activating peptide significantly delays barrier recovery rate. Cockroach and house dust mite allergens, both known to be associated with the development of asthma, allergic rhinitis, and atopic dermatitis, have protease activity, which can activate PAR-2. In this study, we investigated the effects of both allergens on the epidermal barrier function as well as on the epidermal calcium gradient. Both allergens, when topically applied on the barrier-disrupted site, increased protease activities in the epidermis and delayed barrier recovery and lamellar body secretion in murine skin. The topical application of PAR-2-specific antagonist or protease inhibitors normalized the barrier recovery. Cockroach allergens induced intracellular calcium oscillations in cultured human keratinocytes through PAR-2-involved pathway, which was confirmed by desensitization protocol. Abnormal calcium ion distribution after barrier disruption was also observed in allergens-applied skin. These results suggest that allergens with protease activity can influence the epidermal permeability barrier homeostasis through PAR-2 activation and consequent modulation of the calcium ions in skin.

UR - http://www.scopus.com/inward/record.url?scp=47349102541&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47349102541&partnerID=8YFLogxK

U2 - 10.1038/jid.2008.13

DO - 10.1038/jid.2008.13

M3 - Article

VL - 128

SP - 1930

EP - 1939

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 8

ER -