Mitigation of membrane biofouling in MBR using a cellulolytic bacterium, undibacterium sp. DM-1, isolated from activated sludge

Chang Hyun Nahm, Seonki Lee, Sang Hyun Lee, Kibaek Lee, Jaewoo Lee, Hyeokpil Kwon, Kwang Ho Choo, Jung Kee Lee, Jae Young Jang, Chung Hak Lee, Pyungkyu Park

Research output: Contribution to journalArticle

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Abstract

Biofilm formation on the membrane surface results in the loss of permeability in membrane bioreactors (MBRs) for wastewater treatment. Studies have revealed that cellulose is not only produced by a number of bacterial species but also plays a key role during formation of their biofilm. Hence, in this study, cellulase was introduced to a MBR as a cellulose-induced biofilm control strategy. For practical application of cellulase to MBR, a cellulolytic (i.e., cellulase-producing) bacterium, Undibacterium sp. DM-1, was isolated from a lab-scale MBR for wastewater treatment. Prior to its application to MBR, it was confirmed that the cell-free supernatant of DM-1 was capable of inhibiting biofilm formation and of detaching the mature biofilm of activated sludge and cellulose-producing bacteria. This suggested that cellulase could be an effective anti-biofouling agent for MBRs used in wastewater treatment. Undibacterium sp. DM-1-entrapping beads (i.e., cellulolytic-beads) were applied to a continuous MBR to mitigate membrane biofouling 2.2-fold, compared with an MBR with vacant-beads as a control. Subsequent analysis of the cellulose content in the biofilm formed on the membrane surface revealed that this mitigation was associated with an approximately 30% reduction in cellulose by cellulolytic-beads in MBR.

Original languageEnglish
Pages (from-to)573-583
Number of pages11
JournalJournal of Microbiology and Biotechnology
Volume27
Issue number3
DOIs
Publication statusPublished - 2017 Mar 1

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Biofouling
Bioreactors
Sewage
Bacteria
Membranes
Biofilms
Cellulose
Cellulase
Waste Water

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Nahm, Chang Hyun ; Lee, Seonki ; Lee, Sang Hyun ; Lee, Kibaek ; Lee, Jaewoo ; Kwon, Hyeokpil ; Choo, Kwang Ho ; Lee, Jung Kee ; Jang, Jae Young ; Lee, Chung Hak ; Park, Pyungkyu. / Mitigation of membrane biofouling in MBR using a cellulolytic bacterium, undibacterium sp. DM-1, isolated from activated sludge. In: Journal of Microbiology and Biotechnology. 2017 ; Vol. 27, No. 3. pp. 573-583.
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Mitigation of membrane biofouling in MBR using a cellulolytic bacterium, undibacterium sp. DM-1, isolated from activated sludge. / Nahm, Chang Hyun; Lee, Seonki; Lee, Sang Hyun; Lee, Kibaek; Lee, Jaewoo; Kwon, Hyeokpil; Choo, Kwang Ho; Lee, Jung Kee; Jang, Jae Young; Lee, Chung Hak; Park, Pyungkyu.

In: Journal of Microbiology and Biotechnology, Vol. 27, No. 3, 01.03.2017, p. 573-583.

Research output: Contribution to journalArticle

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AU - Nahm, Chang Hyun

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AU - Lee, Sang Hyun

AU - Lee, Kibaek

AU - Lee, Jaewoo

AU - Kwon, Hyeokpil

AU - Choo, Kwang Ho

AU - Lee, Jung Kee

AU - Jang, Jae Young

AU - Lee, Chung Hak

AU - Park, Pyungkyu

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