Mitochondrial ATP synthase is a target for TNBS-induced protein carbonylation in XS-106 dendritic cells

Hwan Je Jeong, Hyung Lee Tae, Hyun Kim Dong, Hun Cho Young, JuHee Lee, SooChan Kim, Sang Kyou Lee, Jaewon Lee, Mingeol Lee

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

ROS are produced in dendritic cells (DCs) during antigen presentation in contact hypersensitivity (CHS). As a result, ROS cause a number of nonenzymatic protein modifications, including carbonylation, which is the most widely used marker of oxidative stress. 2,4,6-Trinitrobenzene sulfonic acid (TNBS) is a well-characterized contact allergen that results in the formation of ROS. However, proteins that are carbonylated in DCs in response to TNBS have not been identified. To study ROS-dependent protein carbonylation in response to TNBS, we used the well-established mouse DC line, XS-106. We focused on the effects of TNBS on oxidation by examining selected oxidative markers. We identified TNBS-induced ROS and myeloperoxidase (MPO) proteins and demonstrated that the increase in ROS resulted in IL-12 production. The increase in oxidation was further confirmed by an oxidation-dependent increase in protein modifications, such as carbonylation. In fact, TNBS strongly induced carbonylation of mitochondrial adenosine triphosphate (ATP) synthase in XS-106 DCs, as determined by MALDI-TOF analysis and 2-D Western blotting. ROS production and protein carbonylation were confirmed in human monocyte-derived DCs (MoDCs). Furthermore, glutathione (GSH) decreased ROS and protein carbonylation in Mo-DCs. Carbonylation of ATP synthase in DCs may contribute to the pathophysiology of CHS.

Original languageEnglish
Pages (from-to)2384-2393
Number of pages10
JournalProteomics
Volume8
Issue number12
DOIs
Publication statusPublished - 2008 Jun 1

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Protein Carbonylation
Carbonylation
Sulfonic Acids
Dendritic Cells
Adenosine Triphosphate
Proteins
Contact Dermatitis
Oxidation
Oxidative stress
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Antigen Presentation
Interleukin-12
sym-trinitrobenzene
Allergens
Peroxidase
Glutathione
Monocytes
Oxidative Stress
Western Blotting
Antigens

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Jeong, Hwan Je ; Tae, Hyung Lee ; Dong, Hyun Kim ; Young, Hun Cho ; Lee, JuHee ; Kim, SooChan ; Lee, Sang Kyou ; Lee, Jaewon ; Lee, Mingeol. / Mitochondrial ATP synthase is a target for TNBS-induced protein carbonylation in XS-106 dendritic cells. In: Proteomics. 2008 ; Vol. 8, No. 12. pp. 2384-2393.
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Mitochondrial ATP synthase is a target for TNBS-induced protein carbonylation in XS-106 dendritic cells. / Jeong, Hwan Je; Tae, Hyung Lee; Dong, Hyun Kim; Young, Hun Cho; Lee, JuHee; Kim, SooChan; Lee, Sang Kyou; Lee, Jaewon; Lee, Mingeol.

In: Proteomics, Vol. 8, No. 12, 01.06.2008, p. 2384-2393.

Research output: Contribution to journalArticle

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T1 - Mitochondrial ATP synthase is a target for TNBS-induced protein carbonylation in XS-106 dendritic cells

AU - Jeong, Hwan Je

AU - Tae, Hyung Lee

AU - Dong, Hyun Kim

AU - Young, Hun Cho

AU - Lee, JuHee

AU - Kim, SooChan

AU - Lee, Sang Kyou

AU - Lee, Jaewon

AU - Lee, Mingeol

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