Mitochondrial Cl--Selective Fluorescent Probe for Biological Applications

Sang Hyun Park, Insu Shin, Young Hyun Kim, Injae Shin

Research output: Contribution to journalArticlepeer-review

Abstract

Herein we describe the development of the first mitochondrial Cl-selective fluorescent probe, Mito-MQAE, and its applications in biological systems. Fluorescence of Mito-MQAE is insensitive to pH over the physiological pH range and is quenched by Cl- with a Stern-Volmer quenching constant of 201 M-1 at pH 7.0. The results of cell studies using Mito-MQAE show that substances with the ability to disrupt mitochondrial membranes cause increases in the mitochondrial Cl- concentration.

Original languageEnglish
Pages (from-to)12116-12119
Number of pages4
JournalAnalytical Chemistry
Volume92
Issue number18
DOIs
Publication statusPublished - 2020 Sep 15

Bibliographical note

Funding Information:
This work was supported financially by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT; Grant No. 2020R1A2C3003462).

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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