Mitochondrial Sirt3 supports cell proliferation by regulating glutamine-dependent oxidation in renal cell carcinoma

Jieun Choi, Eunjin Koh, Yu Shin Lee, Hyun Woo Lee, Hyeok Gu Kang, Young Eun Yoon, WoongKyu Han, Kyung Hwa Choi, Kyung Sup Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Clear cell renal carcinoma (RCC), the most common malignancy arising in the adult kidney, exhibits increased aerobic glycolysis and low mitochondrial respiration due to von Hippel-Lindau gene defects and constitutive hypoxia-inducible factor-α expression. Sirt3 is a major mitochondrial deacetylase that mediates various types of energy metabolism. However, the role of Sirt3 as a tumor suppressor or oncogene in cancer depends on cell types. We show increased Sirt3 expression in the mitochondrial fraction of human RCC tissues. Sirt3 depletion by lentiviral short-hairpin RNA, as well as the stable expression of the inactive mutant of Sirt3, inhibited cell proliferation and tumor growth in xenograft nude mice, respectively. Furthermore, mitochondrial pyruvate, which was used for oxidation in RCC, might be derived from glutamine, but not from glucose and cytosolic pyruvate, due to depletion of mitochondrial pyruvate carrier and the relatively high expression of malic enzyme 2. Depletion of Sirt3 suppressed glutamate dehydrogenase activity, leading to impaired mitochondrial oxygen consumption. Our findings suggest that Sirt3 plays a tumor-progressive role in human RCC by regulating glutamine-derived mitochondrial respiration, particularly in cells where mitochondrial usage of cytosolic pyruvate is severely compromised.

Original languageEnglish
Pages (from-to)547-553
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume474
Issue number3
DOIs
Publication statusPublished - 2016 Jun 3

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Cell proliferation
Glutamine
Pyruvic Acid
Renal Cell Carcinoma
Tumors
Cells
Cell Proliferation
Oxidation
Kidney
Neoplasms
Glutamate Dehydrogenase
Carcinoma
Respiration
Heterografts
Small Interfering RNA
Genes
Tissue
Oxygen
Glucose
Glycolysis

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Choi, Jieun ; Koh, Eunjin ; Lee, Yu Shin ; Lee, Hyun Woo ; Kang, Hyeok Gu ; Yoon, Young Eun ; Han, WoongKyu ; Choi, Kyung Hwa ; Kim, Kyung Sup. / Mitochondrial Sirt3 supports cell proliferation by regulating glutamine-dependent oxidation in renal cell carcinoma. In: Biochemical and Biophysical Research Communications. 2016 ; Vol. 474, No. 3. pp. 547-553.
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Mitochondrial Sirt3 supports cell proliferation by regulating glutamine-dependent oxidation in renal cell carcinoma. / Choi, Jieun; Koh, Eunjin; Lee, Yu Shin; Lee, Hyun Woo; Kang, Hyeok Gu; Yoon, Young Eun; Han, WoongKyu; Choi, Kyung Hwa; Kim, Kyung Sup.

In: Biochemical and Biophysical Research Communications, Vol. 474, No. 3, 03.06.2016, p. 547-553.

Research output: Contribution to journalArticle

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