Abstract
Simple and sensitive assays for DNA detection still represent a highly pursued research area with important implications in biomedical-related sciences. Graphene oxide (GO) is a highly efficient quenching platform for fluorophore-tagged DNA, which is why its use for fluorescent sensing has been widespread over the past decade. GO-based biosensing systems frequently rely upon the isolation of biomolecule-material complexes prior to detection via hybridization-induced desorption of the fluorescent dye. Simple mix-and-read detection formats that do not require purification/isolation/wash steps are envisioned as promising schemes for decentralized analysis, with potential for commercial scalability. For GO-based mix-and-read assays, the aging process of the quenching material in aqueous media can be a crucial parameter affecting the analytical performance, which has so far not been addressed in the literature. To get this goal, top-down characterization microstructures to atomic levels is needed. Herein, we revisit GO as a well-known quenching system, aiming at a centrifugation-free, mix-and-read, no-wash format, toward the detection of an apolipoprotein-E-encoding DNA sequence as a model analyte. We look into the progression of GO aging in water medium through a top-down characterization and investigate the analytical performance of fresh versus aged dispersions in terms of hybridization-based detection. We found that aged GO, while still retaining a high quenching efficiency, undergoes morphological changes over time with concomitant detrimental effects on its analytical performance toward DNA detection.
| Original language | English |
|---|---|
| Pages (from-to) | 1611-1616 |
| Number of pages | 6 |
| Journal | ACS Omega |
| Volume | 4 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2019 Jan 18 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Chemical Engineering(all)
Cite this
}
Mix-and-Read No-Wash Fluorescence DNA Sensing System Using Graphene Oxide : Analytical Performance of Fresh Versus Aged Dispersions. / Manzanares Palenzuela, C. Lorena; Pourrahimi, Amir Masoud; Sofer, Zdeněk; Pumera, Martin.
In: ACS Omega, Vol. 4, No. 1, 18.01.2019, p. 1611-1616.Research output: Contribution to journal › Article
TY - JOUR
T1 - Mix-and-Read No-Wash Fluorescence DNA Sensing System Using Graphene Oxide
T2 - Analytical Performance of Fresh Versus Aged Dispersions
AU - Manzanares Palenzuela, C. Lorena
AU - Pourrahimi, Amir Masoud
AU - Sofer, Zdeněk
AU - Pumera, Martin
PY - 2019/1/18
Y1 - 2019/1/18
N2 - Simple and sensitive assays for DNA detection still represent a highly pursued research area with important implications in biomedical-related sciences. Graphene oxide (GO) is a highly efficient quenching platform for fluorophore-tagged DNA, which is why its use for fluorescent sensing has been widespread over the past decade. GO-based biosensing systems frequently rely upon the isolation of biomolecule-material complexes prior to detection via hybridization-induced desorption of the fluorescent dye. Simple mix-and-read detection formats that do not require purification/isolation/wash steps are envisioned as promising schemes for decentralized analysis, with potential for commercial scalability. For GO-based mix-and-read assays, the aging process of the quenching material in aqueous media can be a crucial parameter affecting the analytical performance, which has so far not been addressed in the literature. To get this goal, top-down characterization microstructures to atomic levels is needed. Herein, we revisit GO as a well-known quenching system, aiming at a centrifugation-free, mix-and-read, no-wash format, toward the detection of an apolipoprotein-E-encoding DNA sequence as a model analyte. We look into the progression of GO aging in water medium through a top-down characterization and investigate the analytical performance of fresh versus aged dispersions in terms of hybridization-based detection. We found that aged GO, while still retaining a high quenching efficiency, undergoes morphological changes over time with concomitant detrimental effects on its analytical performance toward DNA detection.
AB - Simple and sensitive assays for DNA detection still represent a highly pursued research area with important implications in biomedical-related sciences. Graphene oxide (GO) is a highly efficient quenching platform for fluorophore-tagged DNA, which is why its use for fluorescent sensing has been widespread over the past decade. GO-based biosensing systems frequently rely upon the isolation of biomolecule-material complexes prior to detection via hybridization-induced desorption of the fluorescent dye. Simple mix-and-read detection formats that do not require purification/isolation/wash steps are envisioned as promising schemes for decentralized analysis, with potential for commercial scalability. For GO-based mix-and-read assays, the aging process of the quenching material in aqueous media can be a crucial parameter affecting the analytical performance, which has so far not been addressed in the literature. To get this goal, top-down characterization microstructures to atomic levels is needed. Herein, we revisit GO as a well-known quenching system, aiming at a centrifugation-free, mix-and-read, no-wash format, toward the detection of an apolipoprotein-E-encoding DNA sequence as a model analyte. We look into the progression of GO aging in water medium through a top-down characterization and investigate the analytical performance of fresh versus aged dispersions in terms of hybridization-based detection. We found that aged GO, while still retaining a high quenching efficiency, undergoes morphological changes over time with concomitant detrimental effects on its analytical performance toward DNA detection.
UR - http://www.scopus.com/inward/record.url?scp=85060213232&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060213232&partnerID=8YFLogxK
U2 - 10.1021/acsomega.8b02885
DO - 10.1021/acsomega.8b02885
M3 - Article
AN - SCOPUS:85060213232
VL - 4
SP - 1611
EP - 1616
JO - ACS Omega
JF - ACS Omega
SN - 2470-1343
IS - 1
ER -