Mixed-culture H2 fermentation performance and the relation between microbial community composition and hydraulic retention times for a fixed bed reactor fed with galactose/glucose mixtures

Parthiban Anburajan, Jong Hun Park, Periyasamy Sivagurunathan, Arivalagan Pugazhendhi, Gopalakrishnan Kumar, Chang Su Choi, Sang Hyoun Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study examined the mesophilic continuous biohydrogen fermentation from galactose and glucose mixture with an initial substrate concentration of 15 g/L (galactose 12 g/L and glucose 3 g/L) as a resembling carbon source of pretreated red algal hydrolyzate. A fixed bed reactor was fed with the sugar mixture at various hydraulic retention times (HRTs) ranging 12 to 1.5 h. The maximum hydrogen production rate of 52.6 L/L-d was found at 2 h HRT, while the maximum hydrogen yield of 2.3 ± 0.1 mol/mol hexoseadded was achieved at 3 h HRT. Microbial communities and species distribution were analyzed via quantitative polymerase chain reaction (qPCR) and the dominant bacterial population was found as Clostridia followed by Lactobacillus sp. Packing material retained higher 16S rRNA gene copy numbers of total bacteria and Clostridium butyricum fraction compared to fermentation liquor. The finding of the study has demonstrated that H2 production from galactose and glucose mixture could be a viable approach for hydrogen production.

Original languageEnglish
Pages (from-to)339-345
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume124
Issue number3
DOIs
Publication statusPublished - 2017 Sep 1

Fingerprint

Galactose
Fermentation
Glucose
Hydrogen
Clostridium
Hydraulics
Hydrogen production
Clostridium butyricum
Chemical analysis
Gene Dosage
Polymerase chain reaction
Lactobacillus
rRNA Genes
Sugars
Bacteria
Carbon
Genes
Polymerase Chain Reaction
Substrates
Population

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

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title = "Mixed-culture H2 fermentation performance and the relation between microbial community composition and hydraulic retention times for a fixed bed reactor fed with galactose/glucose mixtures",
abstract = "This study examined the mesophilic continuous biohydrogen fermentation from galactose and glucose mixture with an initial substrate concentration of 15 g/L (galactose 12 g/L and glucose 3 g/L) as a resembling carbon source of pretreated red algal hydrolyzate. A fixed bed reactor was fed with the sugar mixture at various hydraulic retention times (HRTs) ranging 12 to 1.5 h. The maximum hydrogen production rate of 52.6 L/L-d was found at 2 h HRT, while the maximum hydrogen yield of 2.3 ± 0.1 mol/mol hexoseadded was achieved at 3 h HRT. Microbial communities and species distribution were analyzed via quantitative polymerase chain reaction (qPCR) and the dominant bacterial population was found as Clostridia followed by Lactobacillus sp. Packing material retained higher 16S rRNA gene copy numbers of total bacteria and Clostridium butyricum fraction compared to fermentation liquor. The finding of the study has demonstrated that H2 production from galactose and glucose mixture could be a viable approach for hydrogen production.",
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Mixed-culture H2 fermentation performance and the relation between microbial community composition and hydraulic retention times for a fixed bed reactor fed with galactose/glucose mixtures. / Anburajan, Parthiban; Park, Jong Hun; Sivagurunathan, Periyasamy; Pugazhendhi, Arivalagan; Kumar, Gopalakrishnan; Choi, Chang Su; Kim, Sang Hyoun.

In: Journal of Bioscience and Bioengineering, Vol. 124, No. 3, 01.09.2017, p. 339-345.

Research output: Contribution to journalArticle

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AU - Pugazhendhi, Arivalagan

AU - Kumar, Gopalakrishnan

AU - Choi, Chang Su

AU - Kim, Sang Hyoun

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