MMP expression profiling in recurred stage IB lung cancer

Namhoon Cho, Kyi Pyo Hong, Sung Hui Hong, Suki Kang, Kyung Young Chung, Sang Ho Cho

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We aimed to clarify the prime role of recurrence in stage I lung cancer. To determine the expression profiles, quantitative RT-PCR and real-time PCR were performed subsequently to evaluate the validity of meaningful molecules identified by 0.12 K c-DNA array experiment surveys. In all, 10 lung cancer patients presenting with recurrence of stage IB were selected and compared with 10 stage IB lung cancer patients without recurrence since biopsied 3 years previously. On c-DNA microarray data analysis using pairs of recurred and the corresponding nonrecurred patients, the following genes were found to be upregulated in the recurred cases: matrix metalloproteinase (MMP)-10 in five cases, MMP-12 in two cases, MMP-11, MMP-14, MMP-15, fos, cyclin E2, E 2F3, TGF-α in each one case. The most frequently upregulated genes in recurred lung cancers were MMP-10 (stromelysin-2) and MMP-12 (macrophage elastase). On transcriptional assay by quantitative RT-PCR and real-time RT-PCR analysis to validate those molecules, both transcripts of MMP-10 and MMP-12 were significantly more upregulated in recurred stage IB lung cancer than in the non-recurred stage IB lung cancer (P = 0.004). Transcript levels were identical to c-DNA array data. The protein levels of these entities were also evaluated by immunohistochemistry of archival slides. By immunohistochemistry, MMP-10 monoclonal antibody showed more intense immunoreactivity in the recurred stage IB lung cancer than in the nonrecurred stage IB lung cancer (P = 0.0313). Our approach revealed that MMP-10 plays an important role in the recurrence in stage IB lung cancer, irrespective of the histologic type.

Original languageEnglish
Pages (from-to)845-851
Number of pages7
JournalOncogene
Volume23
Issue number3
DOIs
Publication statusPublished - 2004 Jan 22

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Matrix Metalloproteinase 10
Matrix Metalloproteinases
Lung Neoplasms
Matrix Metalloproteinase 12
Oligonucleotide Array Sequence Analysis
Recurrence
Matrix Metalloproteinase 15
Real-Time Polymerase Chain Reaction
Matrix Metalloproteinase 11
Immunohistochemistry
Matrix Metalloproteinase 14
Cyclin E
Polymerase Chain Reaction
Pancreatic Elastase
Microarray Analysis
Genes
Macrophages
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Cho, N., Hong, K. P., Hong, S. H., Kang, S., Chung, K. Y., & Cho, S. H. (2004). MMP expression profiling in recurred stage IB lung cancer. Oncogene, 23(3), 845-851. https://doi.org/10.1038/sj.onc.1207140
Cho, Namhoon ; Hong, Kyi Pyo ; Hong, Sung Hui ; Kang, Suki ; Chung, Kyung Young ; Cho, Sang Ho. / MMP expression profiling in recurred stage IB lung cancer. In: Oncogene. 2004 ; Vol. 23, No. 3. pp. 845-851.
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Cho, N, Hong, KP, Hong, SH, Kang, S, Chung, KY & Cho, SH 2004, 'MMP expression profiling in recurred stage IB lung cancer', Oncogene, vol. 23, no. 3, pp. 845-851. https://doi.org/10.1038/sj.onc.1207140

MMP expression profiling in recurred stage IB lung cancer. / Cho, Namhoon; Hong, Kyi Pyo; Hong, Sung Hui; Kang, Suki; Chung, Kyung Young; Cho, Sang Ho.

In: Oncogene, Vol. 23, No. 3, 22.01.2004, p. 845-851.

Research output: Contribution to journalArticle

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Cho N, Hong KP, Hong SH, Kang S, Chung KY, Cho SH. MMP expression profiling in recurred stage IB lung cancer. Oncogene. 2004 Jan 22;23(3):845-851. https://doi.org/10.1038/sj.onc.1207140