Modeling of the kinetic resolution of α-methylbenzylamine with ω-transaminase in a two-liquid-phase system

Jong Shik Shin, Byung Gee Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A mathematical model describing the kinetic resolution of α-methylbenzylamine (α-MBA) in a two-liquid-phase system (water/cyclohexanone) is presented by using ω-transaminase from Bacillus thuringiensis JS64. The model takes into accounts the kinetics of ω-transaminase and the mass transfer of α-MBA and acetophenone between the two phases. The model in the phase separation state at low agitation speeds is extended to that in an emulsion state at high agitation speeds by factoring in faster enzyme inactivation and higher mass transfer rate owing to the increased interfacial area. Model predictions were reasonably consistent with the experimental results in both 2- and 100-ml batch reactors. Various simulation results by using the model indicate that: (1) pH in the aqueous phase should be maintained at 7 because a high aqueous concentration of α-MBA is attained and the optimal pH for enzyme activity appears to be around 7; (2) maximum agitation during the phase separation state is the optimal mixing condition given the two counteracting factors, i.e. mass transfer limitation and interfacial enzyme inactivation; and 3) the optimal volume ratio (V(aq):V(or)) is 4:1. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)426-432
Number of pages7
JournalEnzyme and Microbial Technology
Volume25
Issue number3-5
DOIs
Publication statusPublished - 1999 Aug 1

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Transaminases
Kinetics
Mass transfer
Liquids
Enzymes
Phase separation
Bacillus thuringiensis
Emulsions
Batch reactors
Enzyme activity
Bacilli
Theoretical Models
Water
Mathematical models

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "A mathematical model describing the kinetic resolution of α-methylbenzylamine (α-MBA) in a two-liquid-phase system (water/cyclohexanone) is presented by using ω-transaminase from Bacillus thuringiensis JS64. The model takes into accounts the kinetics of ω-transaminase and the mass transfer of α-MBA and acetophenone between the two phases. The model in the phase separation state at low agitation speeds is extended to that in an emulsion state at high agitation speeds by factoring in faster enzyme inactivation and higher mass transfer rate owing to the increased interfacial area. Model predictions were reasonably consistent with the experimental results in both 2- and 100-ml batch reactors. Various simulation results by using the model indicate that: (1) pH in the aqueous phase should be maintained at 7 because a high aqueous concentration of α-MBA is attained and the optimal pH for enzyme activity appears to be around 7; (2) maximum agitation during the phase separation state is the optimal mixing condition given the two counteracting factors, i.e. mass transfer limitation and interfacial enzyme inactivation; and 3) the optimal volume ratio (V(aq):V(or)) is 4:1. Copyright (C) 1999 Elsevier Science Inc.",
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Modeling of the kinetic resolution of α-methylbenzylamine with ω-transaminase in a two-liquid-phase system. / Shin, Jong Shik; Kim, Byung Gee.

In: Enzyme and Microbial Technology, Vol. 25, No. 3-5, 01.08.1999, p. 426-432.

Research output: Contribution to journalArticle

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