Modified parylene-N films as chemical microenvironments for differentiation and spheroid formation of osteoblast cells

Tae Hun Kim, Jong Sook Lee, Hanhee Jo, Yusun Park, Mijin Yun, Zhiquan Song, Jae Chul Pyun, Misu Lee

Research output: Contribution to journalArticlepeer-review

Abstract

In this work, the influence of parylene N film on the spheroid formation of osteoblast-like cells (MG-63) was determined and compared with that of high-hydrophilicity microenvironments, such as hydrophilic culture matrix and ultraviolet-treated parylene N film. To elucidate the change in cell properties due to the microenvironment of parylene N film, global gene expression profiles of MG-63 cells on parylene N film were analyzed. We confirmed the upregulated expression of osteoblast differentiation- and proliferation-related genes, such as Runx2, ALPL, and BGLAP and MKi67 and PCNA, respectively, using the real-time polymerase chain reaction. In addition, the differentiation and proliferation of osteoblast cells cultured on parylene N film were validated using immunostaining. Finally, the formation of spheroids and regulation of differentiation in human mesenchymal stem cells (MSCs) on parylene N film was demonstrated. The results of this study confirm that the microenvironment with the controlled hydrophobic property of parylene N film could effectively trigger the bone differentiation and maintains the proliferation of MSCs, similar to MG-63 cells without any scaffold structures or physical treatments.

Original languageEnglish
Article number15219
JournalScientific reports
Volume10
Issue number1
DOIs
Publication statusPublished - 2020 Dec 1

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea [Seoul, Korea; grant number NRF-2018R1C1B6003894] and Incheon National University [2017 research grant; Incheon, Korea].

All Science Journal Classification (ASJC) codes

  • General

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