Modulation of matrix metalloproteinase secretion by adenosine A3 receptor in preeclamptic villous explants

Young Han Kim, Han Sung Hwang, YoungTae Kim, Haeng Soo Kim, Yong Won Park

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The impairment of trophoblast invasion associated with preeclampsia may involve matrix metalloproteinase (MMP). Adenosine A3 receptors expression in trophoblast is upregulated in preeclampsia. This study was designed to determine if A3 receptors modulate MMP secretion in villous explants from preeclamptic pregnancies. Villous explants from normal and preeclamptic pregnancies were cultured at 20% and 3% oxygen. There were significantly increased A3 receptor and reduced MMP-2/-9 expression at low oxygen level in normal and preeclamptic explants. Interestingly, in preeclamptic explants, after high oxygen culture, the expression of MMP-2/-9 were recovered to almost same level of those in normal explants. Treatment of preeclamptic explants with A3 receptor agonist in low oxygen level resulted in an enhanced expression of MMP-2/-9 in a time- and dose-dependent manner. In summary, this study indicates that trophoblastic adenosine A3 receptors are 1) modulated by oxygen tension, and 2) regulate MMP-2/-9 expression.

Original languageEnglish
Pages (from-to)939-949
Number of pages11
JournalReproductive Sciences
Volume15
Issue number9
DOIs
Publication statusPublished - 2008 Sep 1

Fingerprint

Adenosine A3 Receptors
Matrix Metalloproteinases
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Oxygen
Trophoblasts
Pre-Eclampsia
Pregnancy

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynaecology

Cite this

Kim, Young Han ; Hwang, Han Sung ; Kim, YoungTae ; Kim, Haeng Soo ; Park, Yong Won. / Modulation of matrix metalloproteinase secretion by adenosine A3 receptor in preeclamptic villous explants. In: Reproductive Sciences. 2008 ; Vol. 15, No. 9. pp. 939-949.
@article{53ace670280e462ebdb38c54f14612d7,
title = "Modulation of matrix metalloproteinase secretion by adenosine A3 receptor in preeclamptic villous explants",
abstract = "The impairment of trophoblast invasion associated with preeclampsia may involve matrix metalloproteinase (MMP). Adenosine A3 receptors expression in trophoblast is upregulated in preeclampsia. This study was designed to determine if A3 receptors modulate MMP secretion in villous explants from preeclamptic pregnancies. Villous explants from normal and preeclamptic pregnancies were cultured at 20{\%} and 3{\%} oxygen. There were significantly increased A3 receptor and reduced MMP-2/-9 expression at low oxygen level in normal and preeclamptic explants. Interestingly, in preeclamptic explants, after high oxygen culture, the expression of MMP-2/-9 were recovered to almost same level of those in normal explants. Treatment of preeclamptic explants with A3 receptor agonist in low oxygen level resulted in an enhanced expression of MMP-2/-9 in a time- and dose-dependent manner. In summary, this study indicates that trophoblastic adenosine A3 receptors are 1) modulated by oxygen tension, and 2) regulate MMP-2/-9 expression.",
author = "Kim, {Young Han} and Hwang, {Han Sung} and YoungTae Kim and Kim, {Haeng Soo} and Park, {Yong Won}",
year = "2008",
month = "9",
day = "1",
doi = "10.1177/1933719108322431",
language = "English",
volume = "15",
pages = "939--949",
journal = "Reproductive Sciences",
issn = "1933-7191",
publisher = "SAGE Publications Inc.",
number = "9",

}

Modulation of matrix metalloproteinase secretion by adenosine A3 receptor in preeclamptic villous explants. / Kim, Young Han; Hwang, Han Sung; Kim, YoungTae; Kim, Haeng Soo; Park, Yong Won.

In: Reproductive Sciences, Vol. 15, No. 9, 01.09.2008, p. 939-949.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Modulation of matrix metalloproteinase secretion by adenosine A3 receptor in preeclamptic villous explants

AU - Kim, Young Han

AU - Hwang, Han Sung

AU - Kim, YoungTae

AU - Kim, Haeng Soo

AU - Park, Yong Won

PY - 2008/9/1

Y1 - 2008/9/1

N2 - The impairment of trophoblast invasion associated with preeclampsia may involve matrix metalloproteinase (MMP). Adenosine A3 receptors expression in trophoblast is upregulated in preeclampsia. This study was designed to determine if A3 receptors modulate MMP secretion in villous explants from preeclamptic pregnancies. Villous explants from normal and preeclamptic pregnancies were cultured at 20% and 3% oxygen. There were significantly increased A3 receptor and reduced MMP-2/-9 expression at low oxygen level in normal and preeclamptic explants. Interestingly, in preeclamptic explants, after high oxygen culture, the expression of MMP-2/-9 were recovered to almost same level of those in normal explants. Treatment of preeclamptic explants with A3 receptor agonist in low oxygen level resulted in an enhanced expression of MMP-2/-9 in a time- and dose-dependent manner. In summary, this study indicates that trophoblastic adenosine A3 receptors are 1) modulated by oxygen tension, and 2) regulate MMP-2/-9 expression.

AB - The impairment of trophoblast invasion associated with preeclampsia may involve matrix metalloproteinase (MMP). Adenosine A3 receptors expression in trophoblast is upregulated in preeclampsia. This study was designed to determine if A3 receptors modulate MMP secretion in villous explants from preeclamptic pregnancies. Villous explants from normal and preeclamptic pregnancies were cultured at 20% and 3% oxygen. There were significantly increased A3 receptor and reduced MMP-2/-9 expression at low oxygen level in normal and preeclamptic explants. Interestingly, in preeclamptic explants, after high oxygen culture, the expression of MMP-2/-9 were recovered to almost same level of those in normal explants. Treatment of preeclamptic explants with A3 receptor agonist in low oxygen level resulted in an enhanced expression of MMP-2/-9 in a time- and dose-dependent manner. In summary, this study indicates that trophoblastic adenosine A3 receptors are 1) modulated by oxygen tension, and 2) regulate MMP-2/-9 expression.

UR - http://www.scopus.com/inward/record.url?scp=57449115129&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57449115129&partnerID=8YFLogxK

U2 - 10.1177/1933719108322431

DO - 10.1177/1933719108322431

M3 - Article

VL - 15

SP - 939

EP - 949

JO - Reproductive Sciences

JF - Reproductive Sciences

SN - 1933-7191

IS - 9

ER -