Abstract
Human mesenchymal stem cells (hMSCs) are multipotent and have been recognized as a source for tissue engineering or cell therapy. It is, therefore, imperative to develop methods to acquire enough hMSCs that maintain self-renewal and differentiation potential. However, aged hMSCs are prone to have a gradual decline in differentiation and proliferation potential with continual cell cycle divisions during in vitro culture. The physiochemical properties of hMSCs are highly dependent on their micro-environment, i.e. the 'stem cell niche'. In this study, the heterogeneous aging of hMSC was examined on chemically defined self-assembly monolayer surfaces. Surface energy was shown to regulate aged hMSC morphology, survival, and proteoglycan expression. High surface energy supplied a preferable environment for hMSC survival and expression of proteoglycans. These results will prove valuable to the design of scaffolds for tissue engineering or for the modulation of implantation environments.
| Original language | English |
|---|---|
| Pages (from-to) | 36-40 |
| Number of pages | 5 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 90 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2012 Feb 1 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
Cite this
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Modulation of the heterogeneous senescence of human mesenchymal stem cells on chemically-modified surfaces. / Kim, Sung Hoon; Lee, Byung Man; Min, Seul Ki; Song, Sun U.; Cho, Jeong Ho; Cho, Kilwon; Shin, Hwa Sung.
In: Colloids and Surfaces B: Biointerfaces, Vol. 90, No. 1, 01.02.2012, p. 36-40.Research output: Contribution to journal › Article
TY - JOUR
T1 - Modulation of the heterogeneous senescence of human mesenchymal stem cells on chemically-modified surfaces
AU - Kim, Sung Hoon
AU - Lee, Byung Man
AU - Min, Seul Ki
AU - Song, Sun U.
AU - Cho, Jeong Ho
AU - Cho, Kilwon
AU - Shin, Hwa Sung
PY - 2012/2/1
Y1 - 2012/2/1
N2 - Human mesenchymal stem cells (hMSCs) are multipotent and have been recognized as a source for tissue engineering or cell therapy. It is, therefore, imperative to develop methods to acquire enough hMSCs that maintain self-renewal and differentiation potential. However, aged hMSCs are prone to have a gradual decline in differentiation and proliferation potential with continual cell cycle divisions during in vitro culture. The physiochemical properties of hMSCs are highly dependent on their micro-environment, i.e. the 'stem cell niche'. In this study, the heterogeneous aging of hMSC was examined on chemically defined self-assembly monolayer surfaces. Surface energy was shown to regulate aged hMSC morphology, survival, and proteoglycan expression. High surface energy supplied a preferable environment for hMSC survival and expression of proteoglycans. These results will prove valuable to the design of scaffolds for tissue engineering or for the modulation of implantation environments.
AB - Human mesenchymal stem cells (hMSCs) are multipotent and have been recognized as a source for tissue engineering or cell therapy. It is, therefore, imperative to develop methods to acquire enough hMSCs that maintain self-renewal and differentiation potential. However, aged hMSCs are prone to have a gradual decline in differentiation and proliferation potential with continual cell cycle divisions during in vitro culture. The physiochemical properties of hMSCs are highly dependent on their micro-environment, i.e. the 'stem cell niche'. In this study, the heterogeneous aging of hMSC was examined on chemically defined self-assembly monolayer surfaces. Surface energy was shown to regulate aged hMSC morphology, survival, and proteoglycan expression. High surface energy supplied a preferable environment for hMSC survival and expression of proteoglycans. These results will prove valuable to the design of scaffolds for tissue engineering or for the modulation of implantation environments.
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UR - http://www.scopus.com/inward/citedby.url?scp=82355180934&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2011.09.035
DO - 10.1016/j.colsurfb.2011.09.035
M3 - Article
VL - 90
SP - 36
EP - 40
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
IS - 1
ER -