Modulatory Effects of Ionized Alkali Mineral Complex (IAMC) on mRNA Expression of Porcine Cytokines

In Soo Choi, Sung Jae Shin, Han Sang Yoo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

It has been recognized that ionized alkali mineral complex (IAMC)-fed farm animals demonstrate higher weight gains hut less incidence of diseases than the unfed ones. However, how these beneficial effects in the IAMC-fed animals are induced has not yet been elucidated clearly. In this study, porcine peripheral blood mononuclear cells (PBMC) were cultured for 4, 24, and 48 hr in the presence of IAMC, and the effects of IAMC on mRNA expression of porcine cytokines were evaluated via a reverse transcription-polymerase chain reaction (RT-PCR). Expression levels of IL-4, IL-6, and IL-10 in IAMC-treated cells were usually higher than those in the untreated ones. However, IAMC-treated cells demonstrated a reduced expression of IL-2. In addition, expression of IFN-γ was generally reduced in the cells treated with IAMC. The expression of IL-12 p35 and IL-12 p40 was not detectable in both the untreated and the IAMC-treated cells. Therefore, these results indicate that IAMC has immunomodulatory effects in vitro on the expression of porcine Th1-and Th2-type cytokines.

Original languageEnglish
Pages (from-to)1179-1182
Number of pages4
JournalJournal of Veterinary Medical Science
Volume63
Issue number11
DOIs
Publication statusPublished - 2001 Nov 1

Fingerprint

Alkalies
alkalis
Minerals
cytokines
Swine
Cytokines
minerals
Messenger RNA
swine
interleukin-12
Interleukin-12
cells
Domestic Animals
interleukin-4
mononuclear leukocytes
interleukin-10
interleukin-2
farmed animal species
interleukin-6
Interleukin-4

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

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abstract = "It has been recognized that ionized alkali mineral complex (IAMC)-fed farm animals demonstrate higher weight gains hut less incidence of diseases than the unfed ones. However, how these beneficial effects in the IAMC-fed animals are induced has not yet been elucidated clearly. In this study, porcine peripheral blood mononuclear cells (PBMC) were cultured for 4, 24, and 48 hr in the presence of IAMC, and the effects of IAMC on mRNA expression of porcine cytokines were evaluated via a reverse transcription-polymerase chain reaction (RT-PCR). Expression levels of IL-4, IL-6, and IL-10 in IAMC-treated cells were usually higher than those in the untreated ones. However, IAMC-treated cells demonstrated a reduced expression of IL-2. In addition, expression of IFN-γ was generally reduced in the cells treated with IAMC. The expression of IL-12 p35 and IL-12 p40 was not detectable in both the untreated and the IAMC-treated cells. Therefore, these results indicate that IAMC has immunomodulatory effects in vitro on the expression of porcine Th1-and Th2-type cytokines.",
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Modulatory Effects of Ionized Alkali Mineral Complex (IAMC) on mRNA Expression of Porcine Cytokines. / Choi, In Soo; Shin, Sung Jae; Yoo, Han Sang.

In: Journal of Veterinary Medical Science, Vol. 63, No. 11, 01.11.2001, p. 1179-1182.

Research output: Contribution to journalArticle

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