Moesin is a biomarker for the assessment of genotoxic carcinogens in mouse lymphoma

Yoen Jung Lee, In Kwon Choi, Yhun Yhong Sheen, Sue Nie Park, Ho Jeong Kwon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

1,2-Dibromoethane and glycidol are well known genotoxic carcinogens, which have been widely used in industry. To identify a specific biomarker for these carcinogens in cells, the cellular proteome of L5178Y mouse lymphoma cells treated with these compounds was analyzed by 2-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry (MS). Of 50 protein spots showing a greater than 1.5-fold increase or decrease in intensity compared to control cells on a 2-D gel, we focused on the candidate biomarker moesin. Western analysis using monoclonal rabbit anti-moesin confirmed the identity of the protein and its increased level of expression upon exposure to the carcinogenic compounds. Moesin expression also increased in cells treated with six additional genotoxic carcinogens, verifying that moesin could serve as a biomarker to monitor phenotypic change upon exposure to genotoxic carcinogens in L5178Y mouse lymphoma cells.

Original languageEnglish
Pages (from-to)203-210
Number of pages8
JournalMolecules and cells
Volume33
Issue number2
DOIs
Publication statusPublished - 2012 Feb 1

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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