1,2-Dibromoethane and glycidol are well known genotoxic carcinogens, which have been widely used in industry. To identify a specific biomarker for these carcinogens in cells, the cellular proteome of L5178Y mouse lymphoma cells treated with these compounds was analyzed by 2-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry (MS). Of 50 protein spots showing a greater than 1.5-fold increase or decrease in intensity compared to control cells on a 2-D gel, we focused on the candidate biomarker moesin. Western analysis using monoclonal rabbit anti-moesin confirmed the identity of the protein and its increased level of expression upon exposure to the carcinogenic compounds. Moesin expression also increased in cells treated with six additional genotoxic carcinogens, verifying that moesin could serve as a biomarker to monitor phenotypic change upon exposure to genotoxic carcinogens in L5178Y mouse lymphoma cells.
Bibliographical noteFunding Information:
This study was partly supported by grants from the Division of Genetic Toxicology National Institute of Toxicological Research, Korea Food & Drug Administration (KFDA), the National Research Foundation of Korea funded by the Korean Government (Ministry of Education, Science, and Technology; 2009-0092964, 2010-0017984, F01-2009-000-10183-0), Translational Research Center for Protein Function Control. The Center for Food and Drug Materials of Agriculture Science & Technology Development (2011-8-1178), Rural Development Administration, National R&D Program, Ministry of Health & Welfare, and from the Brain Korea 21 Project, Republic of Korea. We thank Drs. Young-ki Paik and Jong Shin Yoo for their valuable comments and Korea Basic Science Institute (KBSI) and Yonsei Proteome Research Center (YPRC) for technical support of proteomic analysis.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology