Moesin is a biomarker for the assessment of genotoxic carcinogens in mouse lymphoma

Yoen Jung Lee, In Kwon Choi, Yhun Yhong Sheen, Sue Nie Park, Ho Jeong Kwon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

1,2-Dibromoethane and glycidol are well known genotoxic carcinogens, which have been widely used in industry. To identify a specific biomarker for these carcinogens in cells, the cellular proteome of L5178Y mouse lymphoma cells treated with these compounds was analyzed by 2-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry (MS). Of 50 protein spots showing a greater than 1.5-fold increase or decrease in intensity compared to control cells on a 2-D gel, we focused on the candidate biomarker moesin. Western analysis using monoclonal rabbit anti-moesin confirmed the identity of the protein and its increased level of expression upon exposure to the carcinogenic compounds. Moesin expression also increased in cells treated with six additional genotoxic carcinogens, verifying that moesin could serve as a biomarker to monitor phenotypic change upon exposure to genotoxic carcinogens in L5178Y mouse lymphoma cells.

Original languageEnglish
Pages (from-to)203-210
Number of pages8
JournalMolecules and cells
Volume33
Issue number2
DOIs
Publication statusPublished - 2012 Feb 1

Fingerprint

Carcinogens
Lymphoma
Biomarkers
glycidol
Ethylene Dibromide
Gels
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Proteome
Electrophoresis
Mass Spectrometry
Industry
Proteins
moesin
Rabbits

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Lee, Yoen Jung ; Choi, In Kwon ; Sheen, Yhun Yhong ; Park, Sue Nie ; Kwon, Ho Jeong. / Moesin is a biomarker for the assessment of genotoxic carcinogens in mouse lymphoma. In: Molecules and cells. 2012 ; Vol. 33, No. 2. pp. 203-210.
@article{3e90cbd6bb9244cb85affa6c1347f8c3,
title = "Moesin is a biomarker for the assessment of genotoxic carcinogens in mouse lymphoma",
abstract = "1,2-Dibromoethane and glycidol are well known genotoxic carcinogens, which have been widely used in industry. To identify a specific biomarker for these carcinogens in cells, the cellular proteome of L5178Y mouse lymphoma cells treated with these compounds was analyzed by 2-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry (MS). Of 50 protein spots showing a greater than 1.5-fold increase or decrease in intensity compared to control cells on a 2-D gel, we focused on the candidate biomarker moesin. Western analysis using monoclonal rabbit anti-moesin confirmed the identity of the protein and its increased level of expression upon exposure to the carcinogenic compounds. Moesin expression also increased in cells treated with six additional genotoxic carcinogens, verifying that moesin could serve as a biomarker to monitor phenotypic change upon exposure to genotoxic carcinogens in L5178Y mouse lymphoma cells.",
author = "Lee, {Yoen Jung} and Choi, {In Kwon} and Sheen, {Yhun Yhong} and Park, {Sue Nie} and Kwon, {Ho Jeong}",
year = "2012",
month = "2",
day = "1",
doi = "10.1007/s10059-012-2271-8",
language = "English",
volume = "33",
pages = "203--210",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "2",

}

Moesin is a biomarker for the assessment of genotoxic carcinogens in mouse lymphoma. / Lee, Yoen Jung; Choi, In Kwon; Sheen, Yhun Yhong; Park, Sue Nie; Kwon, Ho Jeong.

In: Molecules and cells, Vol. 33, No. 2, 01.02.2012, p. 203-210.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Moesin is a biomarker for the assessment of genotoxic carcinogens in mouse lymphoma

AU - Lee, Yoen Jung

AU - Choi, In Kwon

AU - Sheen, Yhun Yhong

AU - Park, Sue Nie

AU - Kwon, Ho Jeong

PY - 2012/2/1

Y1 - 2012/2/1

N2 - 1,2-Dibromoethane and glycidol are well known genotoxic carcinogens, which have been widely used in industry. To identify a specific biomarker for these carcinogens in cells, the cellular proteome of L5178Y mouse lymphoma cells treated with these compounds was analyzed by 2-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry (MS). Of 50 protein spots showing a greater than 1.5-fold increase or decrease in intensity compared to control cells on a 2-D gel, we focused on the candidate biomarker moesin. Western analysis using monoclonal rabbit anti-moesin confirmed the identity of the protein and its increased level of expression upon exposure to the carcinogenic compounds. Moesin expression also increased in cells treated with six additional genotoxic carcinogens, verifying that moesin could serve as a biomarker to monitor phenotypic change upon exposure to genotoxic carcinogens in L5178Y mouse lymphoma cells.

AB - 1,2-Dibromoethane and glycidol are well known genotoxic carcinogens, which have been widely used in industry. To identify a specific biomarker for these carcinogens in cells, the cellular proteome of L5178Y mouse lymphoma cells treated with these compounds was analyzed by 2-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry (MS). Of 50 protein spots showing a greater than 1.5-fold increase or decrease in intensity compared to control cells on a 2-D gel, we focused on the candidate biomarker moesin. Western analysis using monoclonal rabbit anti-moesin confirmed the identity of the protein and its increased level of expression upon exposure to the carcinogenic compounds. Moesin expression also increased in cells treated with six additional genotoxic carcinogens, verifying that moesin could serve as a biomarker to monitor phenotypic change upon exposure to genotoxic carcinogens in L5178Y mouse lymphoma cells.

UR - http://www.scopus.com/inward/record.url?scp=84860208698&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860208698&partnerID=8YFLogxK

U2 - 10.1007/s10059-012-2271-8

DO - 10.1007/s10059-012-2271-8

M3 - Article

C2 - 22358511

AN - SCOPUS:84860208698

VL - 33

SP - 203

EP - 210

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 2

ER -