Molecular and biochemical analysis of phthalate and terephthalate degradation by Rhodococcus sp. strain DK17

Young Choi Ki, Dockyu Kim, Jun Sul Woo, Jong Chan Chae, Gerben J. Zylstra, Min Kim Young, Eungbin Kim

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42 Citations (Scopus)

Abstract

Alkylbenzene-degrading Rhodococcus sp. strain DK17 is able to utilize phthalate and terephthalate as growth substrates. The genes encoding the transformation of phthalate and terephthalate to protocatechuate are organized as two separate operons, located 6.7 kb away from each other. Interestingly, both the phthalate and terephthalate operons are induced in response to terephthalate while expression of the terephthalate genes is undetectable in phthalate-grown cells. In addition to two known plasmids (380-kb pDK1 and 330-kb pDK2), a third megaplasmid (750-kb pDK3) was newly identified in DK17. The phthalate and terephthalate operons are duplicated and are present on both pDK2 and pDK3. RT-PCR experiments, coupled with sequence analysis, suggest that phthalate and terephthalate degradation in DK17 proceeds through oxygenation at carbons 3 and 4 and at carbons 1 and 2 to form 3,4-dihydro-3,4- dihydroxyphthalate and 1,2-dihydro-1,2-dihydroxyterephthalate, respectively. The 3,4-dihydroxyphthalate pathway was further corroborated through colorometric tests. Apparently, the two dihydrodiol metabolites are subsequently dehydrogenated and decarboxylated to form protocatechuate, which is further degraded by a protocatechuate 3,4-dioxygenase as confirmed by a ring-cleavage enzyme assay.

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalFEMS Microbiology Letters
Volume252
Issue number2
DOIs
Publication statusPublished - 2005 Nov 15

Bibliographical note

Funding Information:
This work was supported by a grant from KOSEF through the Advanced Environmental Biotechnology Research Center at POSTECH and also by a grant from the Ministry of Science and Technology, Republic of Korea to EK through the 21C Frontier Microbial Genomics and Applications Center Program. GJZ acknowledges the support of NSF through Grant CHE-0221978. We thank Laurie Seliger for excellent DNA sequencing assistance.

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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