Molecular characteristics of colorectal serrated polyps and hyperplastic polyps

A STROBE compliant article

Otgontuya Sambuudash, Heeman Kim, Hannah Jo, Hyun Sik Kim, Kyong Joo Lee, Hong Jun Park, Jae Woo Kim, Meeyon Cho, Hyunsoo Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The serrated neoplasia pathway of colorectal carcinogenesis is characterized by BRAF mutation and aberrant DNA methylation, which have not been reported on Korean patients. The aim of this study was to investigate BRAF mutation and DNA methylation in colorectal serrated polyps and the right colon. Between 2005 and 2013, 146 colon polyps (47 tubular adenomas [TAs], 53 traditional serrated adenomas [TSAs], 17 sessile serrated adenomas/polyps [SSAs], and 29 hyperplastic polyps in the proximal colon [PHPs]) were collected from patients. Paraffinembedded colon polyp tissue was used for DNA extraction. BRAF V600E mutation was identified through polymerase chain reaction (PCR) and pyrosequencing assay. The methylation status of the long interspersed nucleotide element-1, insulin-like growth factor binding protein 7 (IGFBP7), mutL homolog 1 (hMLH1), and CD133 genes were evaluated through disulfite conversion, PCR, and pyrosequencing assay. BRAF V600E mutation was found in 2.1% of TAs, 47.2% of TSAs, 41.2% of SSAs, and 20.7% of PHPs. TSA and SSA had higher BRAF mutation rates than did TA (P<0.0001). TSA had higher BRAF mutation rates than did PHP (P=0.018). IGFBP7 hypermethylation was found in 17% of TAs, 37.7% of TSAs, 88.2% of SSAs, and 37.5% of PHPs. TSA and SSA had higher hypermethylation of IGFBP7 than did TA (P=0.021 and P<0.0001, respectively). SSA had higher hypermethylation of IGFBP7 than did PHP (P=0.002). hMLH1 hypermethylation was found in 2.1% of TAs, 5.7% of TSAs, 0% of SSAs, and 0% of PHPs. CD133 hypermethylation was found in 21.3% of TAs, 9.4% of TSAs, 35.3% of SSAs, and 17.4% of PHPs. BRAF mutation and methylation in TSA and SSA are different from those in PHP in Koreans. These findings suggested that PHP may have different molecular characteristics compared with other serrated polyps.

Original languageEnglish
Pages (from-to)e5592
JournalMedicine (United States)
Volume95
Issue number49
DOIs
Publication statusPublished - 2016 Jan 1

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Polyps
Adenoma
Colon
Mutation
Mutation Rate
DNA Methylation
Methylation
Long Interspersed Nucleotide Elements

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Sambuudash, Otgontuya ; Kim, Heeman ; Jo, Hannah ; Kim, Hyun Sik ; Lee, Kyong Joo ; Park, Hong Jun ; Kim, Jae Woo ; Cho, Meeyon ; Kim, Hyunsoo. / Molecular characteristics of colorectal serrated polyps and hyperplastic polyps : A STROBE compliant article. In: Medicine (United States). 2016 ; Vol. 95, No. 49. pp. e5592.
@article{86e48b2c4d7e40cfb0d42d6d439a8940,
title = "Molecular characteristics of colorectal serrated polyps and hyperplastic polyps: A STROBE compliant article",
abstract = "The serrated neoplasia pathway of colorectal carcinogenesis is characterized by BRAF mutation and aberrant DNA methylation, which have not been reported on Korean patients. The aim of this study was to investigate BRAF mutation and DNA methylation in colorectal serrated polyps and the right colon. Between 2005 and 2013, 146 colon polyps (47 tubular adenomas [TAs], 53 traditional serrated adenomas [TSAs], 17 sessile serrated adenomas/polyps [SSAs], and 29 hyperplastic polyps in the proximal colon [PHPs]) were collected from patients. Paraffinembedded colon polyp tissue was used for DNA extraction. BRAF V600E mutation was identified through polymerase chain reaction (PCR) and pyrosequencing assay. The methylation status of the long interspersed nucleotide element-1, insulin-like growth factor binding protein 7 (IGFBP7), mutL homolog 1 (hMLH1), and CD133 genes were evaluated through disulfite conversion, PCR, and pyrosequencing assay. BRAF V600E mutation was found in 2.1{\%} of TAs, 47.2{\%} of TSAs, 41.2{\%} of SSAs, and 20.7{\%} of PHPs. TSA and SSA had higher BRAF mutation rates than did TA (P<0.0001). TSA had higher BRAF mutation rates than did PHP (P=0.018). IGFBP7 hypermethylation was found in 17{\%} of TAs, 37.7{\%} of TSAs, 88.2{\%} of SSAs, and 37.5{\%} of PHPs. TSA and SSA had higher hypermethylation of IGFBP7 than did TA (P=0.021 and P<0.0001, respectively). SSA had higher hypermethylation of IGFBP7 than did PHP (P=0.002). hMLH1 hypermethylation was found in 2.1{\%} of TAs, 5.7{\%} of TSAs, 0{\%} of SSAs, and 0{\%} of PHPs. CD133 hypermethylation was found in 21.3{\%} of TAs, 9.4{\%} of TSAs, 35.3{\%} of SSAs, and 17.4{\%} of PHPs. BRAF mutation and methylation in TSA and SSA are different from those in PHP in Koreans. These findings suggested that PHP may have different molecular characteristics compared with other serrated polyps.",
author = "Otgontuya Sambuudash and Heeman Kim and Hannah Jo and Kim, {Hyun Sik} and Lee, {Kyong Joo} and Park, {Hong Jun} and Kim, {Jae Woo} and Meeyon Cho and Hyunsoo Kim",
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Molecular characteristics of colorectal serrated polyps and hyperplastic polyps : A STROBE compliant article. / Sambuudash, Otgontuya; Kim, Heeman; Jo, Hannah; Kim, Hyun Sik; Lee, Kyong Joo; Park, Hong Jun; Kim, Jae Woo; Cho, Meeyon; Kim, Hyunsoo.

In: Medicine (United States), Vol. 95, No. 49, 01.01.2016, p. e5592.

Research output: Contribution to journalArticle

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T1 - Molecular characteristics of colorectal serrated polyps and hyperplastic polyps

T2 - A STROBE compliant article

AU - Sambuudash, Otgontuya

AU - Kim, Heeman

AU - Jo, Hannah

AU - Kim, Hyun Sik

AU - Lee, Kyong Joo

AU - Park, Hong Jun

AU - Kim, Jae Woo

AU - Cho, Meeyon

AU - Kim, Hyunsoo

PY - 2016/1/1

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N2 - The serrated neoplasia pathway of colorectal carcinogenesis is characterized by BRAF mutation and aberrant DNA methylation, which have not been reported on Korean patients. The aim of this study was to investigate BRAF mutation and DNA methylation in colorectal serrated polyps and the right colon. Between 2005 and 2013, 146 colon polyps (47 tubular adenomas [TAs], 53 traditional serrated adenomas [TSAs], 17 sessile serrated adenomas/polyps [SSAs], and 29 hyperplastic polyps in the proximal colon [PHPs]) were collected from patients. Paraffinembedded colon polyp tissue was used for DNA extraction. BRAF V600E mutation was identified through polymerase chain reaction (PCR) and pyrosequencing assay. The methylation status of the long interspersed nucleotide element-1, insulin-like growth factor binding protein 7 (IGFBP7), mutL homolog 1 (hMLH1), and CD133 genes were evaluated through disulfite conversion, PCR, and pyrosequencing assay. BRAF V600E mutation was found in 2.1% of TAs, 47.2% of TSAs, 41.2% of SSAs, and 20.7% of PHPs. TSA and SSA had higher BRAF mutation rates than did TA (P<0.0001). TSA had higher BRAF mutation rates than did PHP (P=0.018). IGFBP7 hypermethylation was found in 17% of TAs, 37.7% of TSAs, 88.2% of SSAs, and 37.5% of PHPs. TSA and SSA had higher hypermethylation of IGFBP7 than did TA (P=0.021 and P<0.0001, respectively). SSA had higher hypermethylation of IGFBP7 than did PHP (P=0.002). hMLH1 hypermethylation was found in 2.1% of TAs, 5.7% of TSAs, 0% of SSAs, and 0% of PHPs. CD133 hypermethylation was found in 21.3% of TAs, 9.4% of TSAs, 35.3% of SSAs, and 17.4% of PHPs. BRAF mutation and methylation in TSA and SSA are different from those in PHP in Koreans. These findings suggested that PHP may have different molecular characteristics compared with other serrated polyps.

AB - The serrated neoplasia pathway of colorectal carcinogenesis is characterized by BRAF mutation and aberrant DNA methylation, which have not been reported on Korean patients. The aim of this study was to investigate BRAF mutation and DNA methylation in colorectal serrated polyps and the right colon. Between 2005 and 2013, 146 colon polyps (47 tubular adenomas [TAs], 53 traditional serrated adenomas [TSAs], 17 sessile serrated adenomas/polyps [SSAs], and 29 hyperplastic polyps in the proximal colon [PHPs]) were collected from patients. Paraffinembedded colon polyp tissue was used for DNA extraction. BRAF V600E mutation was identified through polymerase chain reaction (PCR) and pyrosequencing assay. The methylation status of the long interspersed nucleotide element-1, insulin-like growth factor binding protein 7 (IGFBP7), mutL homolog 1 (hMLH1), and CD133 genes were evaluated through disulfite conversion, PCR, and pyrosequencing assay. BRAF V600E mutation was found in 2.1% of TAs, 47.2% of TSAs, 41.2% of SSAs, and 20.7% of PHPs. TSA and SSA had higher BRAF mutation rates than did TA (P<0.0001). TSA had higher BRAF mutation rates than did PHP (P=0.018). IGFBP7 hypermethylation was found in 17% of TAs, 37.7% of TSAs, 88.2% of SSAs, and 37.5% of PHPs. TSA and SSA had higher hypermethylation of IGFBP7 than did TA (P=0.021 and P<0.0001, respectively). SSA had higher hypermethylation of IGFBP7 than did PHP (P=0.002). hMLH1 hypermethylation was found in 2.1% of TAs, 5.7% of TSAs, 0% of SSAs, and 0% of PHPs. CD133 hypermethylation was found in 21.3% of TAs, 9.4% of TSAs, 35.3% of SSAs, and 17.4% of PHPs. BRAF mutation and methylation in TSA and SSA are different from those in PHP in Koreans. These findings suggested that PHP may have different molecular characteristics compared with other serrated polyps.

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