Molecular characterization of Clonorchis sinensis tetraspanin 2 extracellular loop 2

Tae Yun Kim, Eun Joo Chung, Woon Mok Sohn, Sung Hong Hong, Taisoon Yong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In the course of examining EST of the liver fluke Clonorchis sinensis, a cDNA encoding the protein similar to tetraspanin 2 (TSP-2) of blood fluke schistosome was identified as CsTSP2. TSPs are a family of eukaryotic cell membrane-spanning proteins thought to anchor multiple proteins to one area of the cell membrane. Multiple sequence alignment of CsTSP2 revealed over 40% of identities with those of schistosomes. The CCC, PXSC, and CG motives characteristic to extracellular loop 2 (EC2) region of TSP-2 were well conserved. PCR-amplified EC2 of CsTSP2 (CsTSP2EC2) was subcloned into pEXP5 NT/ TOPO bacterial expression plasmid vector. Recombinant CsTSP2EC2 protein (rCsTSP2EC2) fused with 6X His tag was overexpressed bacterially and purified by using Ni- NTA affinity chromatography under denaturing condition. The purified rCsTSP2EC2 was recognized specifically by the sera from human infected with C. sinensis. Considering biological role and specific immunity of TSPs, rCsTSP2EC2 might be a probable vaccine candidate against clonorchiasis. Protective effects of immunizing rCsTSP2EC2 should be further studied.

Original languageEnglish
Pages (from-to)707-711
Number of pages5
JournalParasitology Research
Volume110
Issue number2
DOIs
Publication statusPublished - 2012 Feb 1

Fingerprint

Clonorchis sinensis
Recombinant Proteins
recombinant proteins
Schistosoma
cell membranes
Clonorchiasis
blood flukes
liver flukes
Fasciola hepatica
plasmid vectors
Trematoda
Sequence Alignment
Expressed Sequence Tags
sequence alignment
Eukaryotic Cells
affinity chromatography
Affinity Chromatography
membrane proteins
protective effect
eukaryotic cells

All Science Journal Classification (ASJC) codes

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Kim, Tae Yun ; Chung, Eun Joo ; Sohn, Woon Mok ; Hong, Sung Hong ; Yong, Taisoon. / Molecular characterization of Clonorchis sinensis tetraspanin 2 extracellular loop 2. In: Parasitology Research. 2012 ; Vol. 110, No. 2. pp. 707-711.
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Molecular characterization of Clonorchis sinensis tetraspanin 2 extracellular loop 2. / Kim, Tae Yun; Chung, Eun Joo; Sohn, Woon Mok; Hong, Sung Hong; Yong, Taisoon.

In: Parasitology Research, Vol. 110, No. 2, 01.02.2012, p. 707-711.

Research output: Contribution to journalArticle

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