Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey

Seok Hoon Jeong, Il Kwon Bae, Jung Hun Lee, Seung Ghyu Sohn, Geun Ho Kang, Ghil Ja Jeon, Young Ho Kim, Byeong Chul Jeong, Sang Hee Lee

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Abstract

To determine the prevalence and genotypes of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of Klebsiella pneumoniae and Escherichia coli, we performed antibiotic susceptibility testing, pI determination, induction testing, transconjugation, and DNA sequencing analysis. Among the 509 isolates collected from 13 university hospitals in Korea, 39.2% produced ESBLs. ESBL-producing isolates were detected in every region in Korea. A total of 44.6% of the isolates produced both TEM- and SHV-type ESBLs, and 52% of ESBL-producing isolates transferred resistance to ceftazidime by transconjugation. The ESBLs were TEM-19, TEM-20, TEM-52, SHV-2a, SHV-12, and one new variant identified for the first time in Korea, namely, TEM-116. TEM-1 and SHV-12 were by far the most common variants. TEM-1, TEM-116, and SHV-12 showed a high prevalence in K. pneumoniae. Two isolates (E. coli SH16 and K. pneumoniae SV3) produced CMY-1-like beta-lactamases, which play a decisive role in resistance to cefoxitin and cefotetan, as well as TEM-type enzymes (TEM-20 and TEM-52, respectively). Using MIC patterns and DNA sequencing analysis, we postulated a possible evolution scheme among TEM-type beta-lactamases in Korea: from TEM-1 to TEM-19, from TEM-19 to TEM-20, and from TEM-20 to TEM-52.

Original languageEnglish
Pages (from-to)2902-2906
Number of pages5
JournalJournal of Clinical Microbiology
Volume42
Issue number7
DOIs
Publication statusPublished - 2004 Jul 1

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Klebsiella pneumoniae
beta-Lactamases
Escherichia coli
Korea
DNA Sequence Analysis
Cefotetan
Cefoxitin
Ceftazidime
Surveys and Questionnaires
Genotype
Anti-Bacterial Agents
Enzymes

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Jeong, Seok Hoon ; Bae, Il Kwon ; Lee, Jung Hun ; Sohn, Seung Ghyu ; Kang, Geun Ho ; Jeon, Ghil Ja ; Kim, Young Ho ; Jeong, Byeong Chul ; Lee, Sang Hee. / Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey. In: Journal of Clinical Microbiology. 2004 ; Vol. 42, No. 7. pp. 2902-2906.
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abstract = "To determine the prevalence and genotypes of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of Klebsiella pneumoniae and Escherichia coli, we performed antibiotic susceptibility testing, pI determination, induction testing, transconjugation, and DNA sequencing analysis. Among the 509 isolates collected from 13 university hospitals in Korea, 39.2{\%} produced ESBLs. ESBL-producing isolates were detected in every region in Korea. A total of 44.6{\%} of the isolates produced both TEM- and SHV-type ESBLs, and 52{\%} of ESBL-producing isolates transferred resistance to ceftazidime by transconjugation. The ESBLs were TEM-19, TEM-20, TEM-52, SHV-2a, SHV-12, and one new variant identified for the first time in Korea, namely, TEM-116. TEM-1 and SHV-12 were by far the most common variants. TEM-1, TEM-116, and SHV-12 showed a high prevalence in K. pneumoniae. Two isolates (E. coli SH16 and K. pneumoniae SV3) produced CMY-1-like beta-lactamases, which play a decisive role in resistance to cefoxitin and cefotetan, as well as TEM-type enzymes (TEM-20 and TEM-52, respectively). Using MIC patterns and DNA sequencing analysis, we postulated a possible evolution scheme among TEM-type beta-lactamases in Korea: from TEM-1 to TEM-19, from TEM-19 to TEM-20, and from TEM-20 to TEM-52.",
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Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey. / Jeong, Seok Hoon; Bae, Il Kwon; Lee, Jung Hun; Sohn, Seung Ghyu; Kang, Geun Ho; Jeon, Ghil Ja; Kim, Young Ho; Jeong, Byeong Chul; Lee, Sang Hee.

In: Journal of Clinical Microbiology, Vol. 42, No. 7, 01.07.2004, p. 2902-2906.

Research output: Contribution to journalArticle

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AU - Jeong, Seok Hoon

AU - Bae, Il Kwon

AU - Lee, Jung Hun

AU - Sohn, Seung Ghyu

AU - Kang, Geun Ho

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AU - Kim, Young Ho

AU - Jeong, Byeong Chul

AU - Lee, Sang Hee

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N2 - To determine the prevalence and genotypes of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of Klebsiella pneumoniae and Escherichia coli, we performed antibiotic susceptibility testing, pI determination, induction testing, transconjugation, and DNA sequencing analysis. Among the 509 isolates collected from 13 university hospitals in Korea, 39.2% produced ESBLs. ESBL-producing isolates were detected in every region in Korea. A total of 44.6% of the isolates produced both TEM- and SHV-type ESBLs, and 52% of ESBL-producing isolates transferred resistance to ceftazidime by transconjugation. The ESBLs were TEM-19, TEM-20, TEM-52, SHV-2a, SHV-12, and one new variant identified for the first time in Korea, namely, TEM-116. TEM-1 and SHV-12 were by far the most common variants. TEM-1, TEM-116, and SHV-12 showed a high prevalence in K. pneumoniae. Two isolates (E. coli SH16 and K. pneumoniae SV3) produced CMY-1-like beta-lactamases, which play a decisive role in resistance to cefoxitin and cefotetan, as well as TEM-type enzymes (TEM-20 and TEM-52, respectively). Using MIC patterns and DNA sequencing analysis, we postulated a possible evolution scheme among TEM-type beta-lactamases in Korea: from TEM-1 to TEM-19, from TEM-19 to TEM-20, and from TEM-20 to TEM-52.

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