Molecular characterization of metallo-β-lactamase-producing Acinetobacter baumannii and Acinetobacter genomospecies 3 from Korea: Identification of two new integrons carrying the blaVIM-2 gene cassettes

Jong Hwa Yum, Keonsoo Yi, Hyukmin Lee, Dongeun Yong, Kyungwon Lee, June Myung Kim, Gian Maria Rossolini, Yunsop Chong

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Carbapenem-resistant Acinetobacter spp. used to be rare, but are increasingly isolated in Korea. Among 28 isolates of imipenem-resistant Acinetobacter spp. found in a Korean hospital in 1998 and 1999, 14 produced metallo-β-lactamases. The blaVIM-2 gene was detected, by PCR, in 11 and two isolates of Acinetobacter baumannii and Acinetobacter genomospecies 3, respectively, and blaIMP-1 in one isolate of A. baumannii. The MICs of imipenem for the isolates were 8-32 mg/L. PFGE analysis of SmaI-digested genomic DNA gave identical patterns in eight of 11 blaVIM-2-positive A. baumannii isolates from respiratory specimens of ICU patients. The blaVIM-2 gene cassettes in the isolates are identical to those from Pseudomonas aeruginosa isolates in Europe, but are inserted into new class I integrons In105 and In106. The attC site of the last cassette of the array in In106 is interrupted by the insertion of a putative class II intron. This is the first report of VIM-2 β-lactamase-producing A. baumannii and Acinetobacter genomospecies 3. Production of the VIM-2 enzyme presents an emerging threat of carbapenem resistance among Acinetobacter spp. in Korea.

Original languageEnglish
Pages (from-to)837-840
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume49
Issue number5
Publication statusPublished - 2002 Jun 1

Fingerprint

Integrons
Acinetobacter baumannii
Acinetobacter
Korea
Carbapenems
Imipenem
Genes
Introns
Pseudomonas aeruginosa
Polymerase Chain Reaction
DNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

@article{750eabe4c57e4286a19ab7c509050515,
title = "Molecular characterization of metallo-β-lactamase-producing Acinetobacter baumannii and Acinetobacter genomospecies 3 from Korea: Identification of two new integrons carrying the blaVIM-2 gene cassettes",
abstract = "Carbapenem-resistant Acinetobacter spp. used to be rare, but are increasingly isolated in Korea. Among 28 isolates of imipenem-resistant Acinetobacter spp. found in a Korean hospital in 1998 and 1999, 14 produced metallo-β-lactamases. The blaVIM-2 gene was detected, by PCR, in 11 and two isolates of Acinetobacter baumannii and Acinetobacter genomospecies 3, respectively, and blaIMP-1 in one isolate of A. baumannii. The MICs of imipenem for the isolates were 8-32 mg/L. PFGE analysis of SmaI-digested genomic DNA gave identical patterns in eight of 11 blaVIM-2-positive A. baumannii isolates from respiratory specimens of ICU patients. The blaVIM-2 gene cassettes in the isolates are identical to those from Pseudomonas aeruginosa isolates in Europe, but are inserted into new class I integrons In105 and In106. The attC site of the last cassette of the array in In106 is interrupted by the insertion of a putative class II intron. This is the first report of VIM-2 β-lactamase-producing A. baumannii and Acinetobacter genomospecies 3. Production of the VIM-2 enzyme presents an emerging threat of carbapenem resistance among Acinetobacter spp. in Korea.",
author = "Yum, {Jong Hwa} and Keonsoo Yi and Hyukmin Lee and Dongeun Yong and Kyungwon Lee and Kim, {June Myung} and Rossolini, {Gian Maria} and Yunsop Chong",
year = "2002",
month = "6",
day = "1",
language = "English",
volume = "49",
pages = "837--840",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "5",

}

Molecular characterization of metallo-β-lactamase-producing Acinetobacter baumannii and Acinetobacter genomospecies 3 from Korea : Identification of two new integrons carrying the blaVIM-2 gene cassettes. / Yum, Jong Hwa; Yi, Keonsoo; Lee, Hyukmin; Yong, Dongeun; Lee, Kyungwon; Kim, June Myung; Rossolini, Gian Maria; Chong, Yunsop.

In: Journal of Antimicrobial Chemotherapy, Vol. 49, No. 5, 01.06.2002, p. 837-840.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Molecular characterization of metallo-β-lactamase-producing Acinetobacter baumannii and Acinetobacter genomospecies 3 from Korea

T2 - Identification of two new integrons carrying the blaVIM-2 gene cassettes

AU - Yum, Jong Hwa

AU - Yi, Keonsoo

AU - Lee, Hyukmin

AU - Yong, Dongeun

AU - Lee, Kyungwon

AU - Kim, June Myung

AU - Rossolini, Gian Maria

AU - Chong, Yunsop

PY - 2002/6/1

Y1 - 2002/6/1

N2 - Carbapenem-resistant Acinetobacter spp. used to be rare, but are increasingly isolated in Korea. Among 28 isolates of imipenem-resistant Acinetobacter spp. found in a Korean hospital in 1998 and 1999, 14 produced metallo-β-lactamases. The blaVIM-2 gene was detected, by PCR, in 11 and two isolates of Acinetobacter baumannii and Acinetobacter genomospecies 3, respectively, and blaIMP-1 in one isolate of A. baumannii. The MICs of imipenem for the isolates were 8-32 mg/L. PFGE analysis of SmaI-digested genomic DNA gave identical patterns in eight of 11 blaVIM-2-positive A. baumannii isolates from respiratory specimens of ICU patients. The blaVIM-2 gene cassettes in the isolates are identical to those from Pseudomonas aeruginosa isolates in Europe, but are inserted into new class I integrons In105 and In106. The attC site of the last cassette of the array in In106 is interrupted by the insertion of a putative class II intron. This is the first report of VIM-2 β-lactamase-producing A. baumannii and Acinetobacter genomospecies 3. Production of the VIM-2 enzyme presents an emerging threat of carbapenem resistance among Acinetobacter spp. in Korea.

AB - Carbapenem-resistant Acinetobacter spp. used to be rare, but are increasingly isolated in Korea. Among 28 isolates of imipenem-resistant Acinetobacter spp. found in a Korean hospital in 1998 and 1999, 14 produced metallo-β-lactamases. The blaVIM-2 gene was detected, by PCR, in 11 and two isolates of Acinetobacter baumannii and Acinetobacter genomospecies 3, respectively, and blaIMP-1 in one isolate of A. baumannii. The MICs of imipenem for the isolates were 8-32 mg/L. PFGE analysis of SmaI-digested genomic DNA gave identical patterns in eight of 11 blaVIM-2-positive A. baumannii isolates from respiratory specimens of ICU patients. The blaVIM-2 gene cassettes in the isolates are identical to those from Pseudomonas aeruginosa isolates in Europe, but are inserted into new class I integrons In105 and In106. The attC site of the last cassette of the array in In106 is interrupted by the insertion of a putative class II intron. This is the first report of VIM-2 β-lactamase-producing A. baumannii and Acinetobacter genomospecies 3. Production of the VIM-2 enzyme presents an emerging threat of carbapenem resistance among Acinetobacter spp. in Korea.

UR - http://www.scopus.com/inward/record.url?scp=0036001185&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036001185&partnerID=8YFLogxK

M3 - Article

C2 - 12003980

AN - SCOPUS:0036001185

VL - 49

SP - 837

EP - 840

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 5

ER -