Molecular characterization of NbCHMP1 encoding a homolog of human CHMP1 in Nicotiana benthamiana

Kyoung Sil Yang, Un Ho Jin, Junwon Kim, Kiwon Song, Soo Jin Kim, Inhwan Hwang, Yong Pyo Lim, Hyun Sook Pai

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In humans, CHMP1 encodes a protein of dual function that plays a role in both modification of chromatin structure and endosomal vesicle trafficking. Recently, it was found that sal1, a CHMP1 homolog in maize, is important for the development of the aleurone cell layers in maize endosperm. In this study, we investigated the structure and function of a Nicotiana benthamiana CHMP1 homolog designated NbCHMP1. NbCHMP1 encodes a small protein with a bipartite nuclear localization signal at its N-terminus, and good homology with the corresponding genes from diverse plants and animals. NbCHMP1 mRNA was present at comparable levels in stems, roots, flowers, and leaves. A GFP fusion of the full length NbCHMP1 protein was localized to the cytosol in distinct structures, while a GFP fusion of its N-terminal 80 aa was targeted to the nucleus, suggesting dual-targeting of NbCHMP1 in plants. Overexpression of NbCHMP1 in yeast did not affect its growth and the expressed protein was present in the cytosol in particulate form. Virus-induced gene silencing of NbCHMP1 resulted in subtle alterations of leaf morphology and color, without significantly affecting plant viability or development. Thus the CHMP1 homolog apparently does not play an essential role in the development of the vegetative tissues of N. benthamiana, in contrast to the essential role of sal1 in formation of the aleurone cell layers during maize endosperm development.

Original languageEnglish
Pages (from-to)255-261
Number of pages7
JournalMolecules and Cells
Volume17
Issue number2
Publication statusPublished - 2004 Apr 30

Fingerprint

Tobacco
Zea mays
Endosperm
Cytosol
Proteins
Nuclear Localization Signals
Plant Genes
Gene Silencing
Chromatin
Color
Yeasts
Viruses
Messenger RNA
Growth

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Yang, Kyoung Sil ; Jin, Un Ho ; Kim, Junwon ; Song, Kiwon ; Kim, Soo Jin ; Hwang, Inhwan ; Lim, Yong Pyo ; Pai, Hyun Sook. / Molecular characterization of NbCHMP1 encoding a homolog of human CHMP1 in Nicotiana benthamiana. In: Molecules and Cells. 2004 ; Vol. 17, No. 2. pp. 255-261.
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abstract = "In humans, CHMP1 encodes a protein of dual function that plays a role in both modification of chromatin structure and endosomal vesicle trafficking. Recently, it was found that sal1, a CHMP1 homolog in maize, is important for the development of the aleurone cell layers in maize endosperm. In this study, we investigated the structure and function of a Nicotiana benthamiana CHMP1 homolog designated NbCHMP1. NbCHMP1 encodes a small protein with a bipartite nuclear localization signal at its N-terminus, and good homology with the corresponding genes from diverse plants and animals. NbCHMP1 mRNA was present at comparable levels in stems, roots, flowers, and leaves. A GFP fusion of the full length NbCHMP1 protein was localized to the cytosol in distinct structures, while a GFP fusion of its N-terminal 80 aa was targeted to the nucleus, suggesting dual-targeting of NbCHMP1 in plants. Overexpression of NbCHMP1 in yeast did not affect its growth and the expressed protein was present in the cytosol in particulate form. Virus-induced gene silencing of NbCHMP1 resulted in subtle alterations of leaf morphology and color, without significantly affecting plant viability or development. Thus the CHMP1 homolog apparently does not play an essential role in the development of the vegetative tissues of N. benthamiana, in contrast to the essential role of sal1 in formation of the aleurone cell layers during maize endosperm development.",
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Yang, KS, Jin, UH, Kim, J, Song, K, Kim, SJ, Hwang, I, Lim, YP & Pai, HS 2004, 'Molecular characterization of NbCHMP1 encoding a homolog of human CHMP1 in Nicotiana benthamiana', Molecules and Cells, vol. 17, no. 2, pp. 255-261.

Molecular characterization of NbCHMP1 encoding a homolog of human CHMP1 in Nicotiana benthamiana. / Yang, Kyoung Sil; Jin, Un Ho; Kim, Junwon; Song, Kiwon; Kim, Soo Jin; Hwang, Inhwan; Lim, Yong Pyo; Pai, Hyun Sook.

In: Molecules and Cells, Vol. 17, No. 2, 30.04.2004, p. 255-261.

Research output: Contribution to journalArticle

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T1 - Molecular characterization of NbCHMP1 encoding a homolog of human CHMP1 in Nicotiana benthamiana

AU - Yang, Kyoung Sil

AU - Jin, Un Ho

AU - Kim, Junwon

AU - Song, Kiwon

AU - Kim, Soo Jin

AU - Hwang, Inhwan

AU - Lim, Yong Pyo

AU - Pai, Hyun Sook

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N2 - In humans, CHMP1 encodes a protein of dual function that plays a role in both modification of chromatin structure and endosomal vesicle trafficking. Recently, it was found that sal1, a CHMP1 homolog in maize, is important for the development of the aleurone cell layers in maize endosperm. In this study, we investigated the structure and function of a Nicotiana benthamiana CHMP1 homolog designated NbCHMP1. NbCHMP1 encodes a small protein with a bipartite nuclear localization signal at its N-terminus, and good homology with the corresponding genes from diverse plants and animals. NbCHMP1 mRNA was present at comparable levels in stems, roots, flowers, and leaves. A GFP fusion of the full length NbCHMP1 protein was localized to the cytosol in distinct structures, while a GFP fusion of its N-terminal 80 aa was targeted to the nucleus, suggesting dual-targeting of NbCHMP1 in plants. Overexpression of NbCHMP1 in yeast did not affect its growth and the expressed protein was present in the cytosol in particulate form. Virus-induced gene silencing of NbCHMP1 resulted in subtle alterations of leaf morphology and color, without significantly affecting plant viability or development. Thus the CHMP1 homolog apparently does not play an essential role in the development of the vegetative tissues of N. benthamiana, in contrast to the essential role of sal1 in formation of the aleurone cell layers during maize endosperm development.

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