Molecular characterization of NbPAF encoding the α6 subunit of the 20S proteasome in Nicotiana benthamiana

Moonil Kim, Kyoung Sil Yang, Yu Kyung Kim, Kyung Hee Paek, Hyunsook Pai

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The 26S proteasome involved in degradation of proteins covalently modified with polyubiquitin consists of the 20S proteasome and 19S regulatory complex. The NbPAF gene encoding the α6 subunit of the 20S proteasome was identified from Nicotiana benthamiana. NbPAF exhibits high sequence homology with the corresponding genes from Arabidopsis, human and yeast. The deduced amino acid sequence of NbPAF reveals that this protein contains the proteasome α-type subunits signature and nuclear localization signal at the N-terminus. The genomic Southern blot analysis suggests that the N. benthamiana genome contains one copy of NbPAF. The NbPAF mRNA was detected abundantly in flowers and weakly in roots and stems, but it was almost undetectable in mature leaves. In response to stresses, accumulation of the NbPAF mRNA was stimulated by methyl jasmonate, NaCl and salicylic acid, but not by abscisic acid and cold treatment in leaves. The NbPAF-GFP fusion protein was localized in the cytoplasm and nucleus.

Original languageEnglish
Pages (from-to)127-132
Number of pages6
JournalMolecules and cells
Volume15
Issue number1
Publication statusPublished - 2003 Feb 1

Fingerprint

Proteasome Endopeptidase Complex
Tobacco
Polyubiquitin
Nuclear Localization Signals
Abscisic Acid
Messenger RNA
Salicylic Acid
Sequence Homology
Southern Blotting
Arabidopsis
Proteolysis
Genes
Amino Acid Sequence
Cytoplasm
Proteins
Yeasts
Genome

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Kim, Moonil ; Yang, Kyoung Sil ; Kim, Yu Kyung ; Paek, Kyung Hee ; Pai, Hyunsook. / Molecular characterization of NbPAF encoding the α6 subunit of the 20S proteasome in Nicotiana benthamiana. In: Molecules and cells. 2003 ; Vol. 15, No. 1. pp. 127-132.
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Molecular characterization of NbPAF encoding the α6 subunit of the 20S proteasome in Nicotiana benthamiana. / Kim, Moonil; Yang, Kyoung Sil; Kim, Yu Kyung; Paek, Kyung Hee; Pai, Hyunsook.

In: Molecules and cells, Vol. 15, No. 1, 01.02.2003, p. 127-132.

Research output: Contribution to journalArticle

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