Abstract
In this study, we investigated the cellular functions of nuclear localizing protein (NLP) in Nicotiana benthamiana. The GFP fusion protein of Arabidopsis NLP is mostly targeted to the nucleus and cytosol, but it infrequently targets only the nucleus. Silencing of NLP using virus-induced gene silencing caused growth retardation and abnormal leaf development in N. benthamiana. At the cellular level, downregulation of NLP expression increased the size of pavement cells and stomata in the leaf tissues. NLP suppression also increased endoreduplication in leaf cells, suggesting a role of NLP in the endocycle. NLP transcript levels in leaves were significantly increased in response to infection with the nonhost pathogens. NLP deficiency attenuated HR cell death against nonhost pathogens and in response to gene-for-gene interaction. These findings suggest that NLP plays a role in plant development and plant defense responses against pathogens.
| Original language | English |
|---|---|
| Pages (from-to) | 199-208 |
| Number of pages | 10 |
| Journal | Journal of Plant Biology |
| Volume | 54 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2011 Jun 1 |
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All Science Journal Classification (ASJC) codes
- Plant Science
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Molecular Characterization of NLP Function in Nicotiana benthamiana. / Ahn, Eu Ree; Sarowar, Sujon; Jeon, Young; Pai, Hyun Sook.
In: Journal of Plant Biology, Vol. 54, No. 3, 01.06.2011, p. 199-208.Research output: Contribution to journal › Article
TY - JOUR
T1 - Molecular Characterization of NLP Function in Nicotiana benthamiana
AU - Ahn, Eu Ree
AU - Sarowar, Sujon
AU - Jeon, Young
AU - Pai, Hyun Sook
PY - 2011/6/1
Y1 - 2011/6/1
N2 - In this study, we investigated the cellular functions of nuclear localizing protein (NLP) in Nicotiana benthamiana. The GFP fusion protein of Arabidopsis NLP is mostly targeted to the nucleus and cytosol, but it infrequently targets only the nucleus. Silencing of NLP using virus-induced gene silencing caused growth retardation and abnormal leaf development in N. benthamiana. At the cellular level, downregulation of NLP expression increased the size of pavement cells and stomata in the leaf tissues. NLP suppression also increased endoreduplication in leaf cells, suggesting a role of NLP in the endocycle. NLP transcript levels in leaves were significantly increased in response to infection with the nonhost pathogens. NLP deficiency attenuated HR cell death against nonhost pathogens and in response to gene-for-gene interaction. These findings suggest that NLP plays a role in plant development and plant defense responses against pathogens.
AB - In this study, we investigated the cellular functions of nuclear localizing protein (NLP) in Nicotiana benthamiana. The GFP fusion protein of Arabidopsis NLP is mostly targeted to the nucleus and cytosol, but it infrequently targets only the nucleus. Silencing of NLP using virus-induced gene silencing caused growth retardation and abnormal leaf development in N. benthamiana. At the cellular level, downregulation of NLP expression increased the size of pavement cells and stomata in the leaf tissues. NLP suppression also increased endoreduplication in leaf cells, suggesting a role of NLP in the endocycle. NLP transcript levels in leaves were significantly increased in response to infection with the nonhost pathogens. NLP deficiency attenuated HR cell death against nonhost pathogens and in response to gene-for-gene interaction. These findings suggest that NLP plays a role in plant development and plant defense responses against pathogens.
UR - http://www.scopus.com/inward/record.url?scp=79955572329&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79955572329&partnerID=8YFLogxK
U2 - 10.1007/s12374-011-9156-9
DO - 10.1007/s12374-011-9156-9
M3 - Article
AN - SCOPUS:79955572329
VL - 54
SP - 199
EP - 208
JO - Journal of Plant Biology
JF - Journal of Plant Biology
SN - 1226-9239
IS - 3
ER -