Molecular Characterization of NLP Function in Nicotiana benthamiana

Eu Ree Ahn, Sujon Sarowar, Young Jeon, Hyun Sook Pai

Research output: Contribution to journalArticle

Abstract

In this study, we investigated the cellular functions of nuclear localizing protein (NLP) in Nicotiana benthamiana. The GFP fusion protein of Arabidopsis NLP is mostly targeted to the nucleus and cytosol, but it infrequently targets only the nucleus. Silencing of NLP using virus-induced gene silencing caused growth retardation and abnormal leaf development in N. benthamiana. At the cellular level, downregulation of NLP expression increased the size of pavement cells and stomata in the leaf tissues. NLP suppression also increased endoreduplication in leaf cells, suggesting a role of NLP in the endocycle. NLP transcript levels in leaves were significantly increased in response to infection with the nonhost pathogens. NLP deficiency attenuated HR cell death against nonhost pathogens and in response to gene-for-gene interaction. These findings suggest that NLP plays a role in plant development and plant defense responses against pathogens.

Original languageEnglish
Pages (from-to)199-208
Number of pages10
JournalJournal of Plant Biology
Volume54
Issue number3
DOIs
Publication statusPublished - 2011 Jun 1

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Nicotiana benthamiana
nuclear proteins
pathogens
leaves
protein deficiencies
endopolyploidy
gene interaction
leaf development
gene silencing
cytosol
stomata
growth retardation
plant development
cell death
protein synthesis
Arabidopsis
cells
viruses

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Ahn, Eu Ree ; Sarowar, Sujon ; Jeon, Young ; Pai, Hyun Sook. / Molecular Characterization of NLP Function in Nicotiana benthamiana. In: Journal of Plant Biology. 2011 ; Vol. 54, No. 3. pp. 199-208.
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Molecular Characterization of NLP Function in Nicotiana benthamiana. / Ahn, Eu Ree; Sarowar, Sujon; Jeon, Young; Pai, Hyun Sook.

In: Journal of Plant Biology, Vol. 54, No. 3, 01.06.2011, p. 199-208.

Research output: Contribution to journalArticle

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