Molecular characterization of TEM-type β-lactamases identified in cold-seep sediments of edison seamount (South of Lihir Island, Papua New Guinea)

Seok Song Jae, Ho Jeon Jeong, Hun Lee Jung, Hoon Jeong Seok, Chul Jeong Byeong, Sang Jin Kim, Jung Hyun Lee, Hee Lee Sang

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

To determine the prevalence and genotypes of β-lactamases among lones of a metagenomic library from the cold-seep sediments of Edison seamount (10,000 years old), we performed pulse-field gel electrophoresis, antibiotic susceptibility testing, pI determination, and DNA sequencing analysis. Among the 8,823 clones of the library, thirty clones produced ß-lactamases and had high levels of genetic diversity. Consistent with minimum inhibitory concentration patterns, we found that five (16.7%) of thirty clones produced an extended-spectrum β-lactamase. 837- and 259-bp fragments specific to blaTEM genes were amplified, as determined by banding patterns of PCR amplification with designed primers. TEM-1 1 was the most prevalent β-lactamase and conferred resistance to ampicillin, piperacillin, and cephalothin. TEM-116 had a spectrum that was extended to ceftazidime, cefotaxime, and aztreonam. The resistance levels conferred by the pre-antibiotic era alleles of TEM-type β-lactamases were essentially the same as the resistance levels conferred by the TEM-type alleles which had been isolated from clinically resistant strains of bacteria of the antibiotic era. Our first report on TEM-type β-lactamases of the pre-antibiotic era indicates that TEM-type β-lactamases paint a picture in which most of the diversity of the enzymes may not be the result of recent evolution, but that of ancient evolution.

Original languageEnglish
Pages (from-to)172-178
Number of pages7
JournalJournal of Microbiology
Volume43
Issue number2
Publication statusPublished - 2005 Apr 1

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Papua New Guinea
Islands
Anti-Bacterial Agents
Clone Cells
Alleles
Ampicillin Resistance
Aztreonam
Cephalothin
Metagenomics
Piperacillin
Paint
Ceftazidime
Cefotaxime
Microbial Sensitivity Tests
DNA Sequence Analysis
Libraries
Electrophoresis
Gels
Genotype
Bacteria

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Jae, Seok Song ; Jeong, Ho Jeon ; Jung, Hun Lee ; Seok, Hoon Jeong ; Byeong, Chul Jeong ; Kim, Sang Jin ; Lee, Jung Hyun ; Sang, Hee Lee. / Molecular characterization of TEM-type β-lactamases identified in cold-seep sediments of edison seamount (South of Lihir Island, Papua New Guinea). In: Journal of Microbiology. 2005 ; Vol. 43, No. 2. pp. 172-178.
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abstract = "To determine the prevalence and genotypes of β-lactamases among lones of a metagenomic library from the cold-seep sediments of Edison seamount (10,000 years old), we performed pulse-field gel electrophoresis, antibiotic susceptibility testing, pI determination, and DNA sequencing analysis. Among the 8,823 clones of the library, thirty clones produced {\ss}-lactamases and had high levels of genetic diversity. Consistent with minimum inhibitory concentration patterns, we found that five (16.7{\%}) of thirty clones produced an extended-spectrum β-lactamase. 837- and 259-bp fragments specific to blaTEM genes were amplified, as determined by banding patterns of PCR amplification with designed primers. TEM-1 1 was the most prevalent β-lactamase and conferred resistance to ampicillin, piperacillin, and cephalothin. TEM-116 had a spectrum that was extended to ceftazidime, cefotaxime, and aztreonam. The resistance levels conferred by the pre-antibiotic era alleles of TEM-type β-lactamases were essentially the same as the resistance levels conferred by the TEM-type alleles which had been isolated from clinically resistant strains of bacteria of the antibiotic era. Our first report on TEM-type β-lactamases of the pre-antibiotic era indicates that TEM-type β-lactamases paint a picture in which most of the diversity of the enzymes may not be the result of recent evolution, but that of ancient evolution.",
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Molecular characterization of TEM-type β-lactamases identified in cold-seep sediments of edison seamount (South of Lihir Island, Papua New Guinea). / Jae, Seok Song; Jeong, Ho Jeon; Jung, Hun Lee; Seok, Hoon Jeong; Byeong, Chul Jeong; Kim, Sang Jin; Lee, Jung Hyun; Sang, Hee Lee.

In: Journal of Microbiology, Vol. 43, No. 2, 01.04.2005, p. 172-178.

Research output: Contribution to journalArticle

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