Molecular Characterization of the NelF2Bβ Gene Encoding a Putative elF2B β-Subunit in Nicotiana tabacum

Moon Il Kim, Seong Whan Park, Sung Hoon Yu, Hye Sun Cho, Hyun Jung Ha, Inhwan Hwang, Hyun Sook Pai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The NeIF2Bβ cDNA encoding β-subunit of the translation initiation factor 2B (eIF2B-β) was identified from Nicotiana tabacum through protein interaction with PRK1, a reproductive-organ-specific receptor-like kinase (Park et al., 2000). The eIF2B is a guanine nucleotide-exchange protein that consists of five subunits, which function in the regulation of translation in eukaryotic cells. The NeIF2Bβ that shows a high homology in the amino acid sequence with other β-subunits also exhibits sequence similarity to a and δ subunits of eIF2B from yeast and animals. The NeIF2Bβ gene was expressed in all of the tissues examined, but the mRNA level was higher in reproductive tissues than in vegetative tissues. During anther development, the NeIF2Bβ mRNA was detected in all stages with a slightly higher level in the earliest stage. The NeIF2Bβ-GFP fusion protein was mainly localized in the cytosol.

Original languageEnglish
Pages (from-to)110-114
Number of pages5
JournalMolecules and cells
Volume11
Issue number1
Publication statusPublished - 2001 Feb 28

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Molecular Characterization of the NelF2Bβ Gene Encoding a Putative elF2B β-Subunit in Nicotiana tabacum'. Together they form a unique fingerprint.

  • Cite this

    Kim, M. I., Park, S. W., Yu, S. H., Cho, H. S., Ha, H. J., Hwang, I., & Pai, H. S. (2001). Molecular Characterization of the NelF2Bβ Gene Encoding a Putative elF2B β-Subunit in Nicotiana tabacum. Molecules and cells, 11(1), 110-114.