Molecular cloning and anti-fungal effect of endo-β-1,3-glucanase from Thermotoga maritima

Chang Bin Woo, Hae Na Kang, Soo Bok Lee

Research output: Contribution to journalEditorial

4 Citations (Scopus)

Abstract

A gene encoding an endo-β-1,3-glucanase from Thermotoga maritima MSB8 (TmβG) was cloned and expressed in Escherichia coli. The purified enzyme produced various β-1,3-glucooligosaccharides from soluble laminarin, and mainly β-1,3-glucooligosaccharides smaller than laminaritetrose from insoluble curdlan. The optimum pH and temperature of the enzyme were 5.0 and 80°C, respectively. TmβG inhibited the growth of Candida albicans, which indicates that the enzyme could potentially be used as an anti-fungal agent to control invasive infections.

Original languageEnglish
Pages (from-to)1243-1246
Number of pages4
JournalFood Science and Biotechnology
Volume23
Issue number4
DOIs
Publication statusPublished - 2014 Aug

Fingerprint

Thermotoga maritima
Molecular Cloning
molecular cloning
Enzymes
enzymes
curdlan
Infection Control
Candida albicans
Escherichia coli
Temperature
Growth
infection
Genes
temperature
genes
glucooligosaccharides

All Science Journal Classification (ASJC) codes

  • Food Science
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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title = "Molecular cloning and anti-fungal effect of endo-β-1,3-glucanase from Thermotoga maritima",
abstract = "A gene encoding an endo-β-1,3-glucanase from Thermotoga maritima MSB8 (TmβG) was cloned and expressed in Escherichia coli. The purified enzyme produced various β-1,3-glucooligosaccharides from soluble laminarin, and mainly β-1,3-glucooligosaccharides smaller than laminaritetrose from insoluble curdlan. The optimum pH and temperature of the enzyme were 5.0 and 80°C, respectively. TmβG inhibited the growth of Candida albicans, which indicates that the enzyme could potentially be used as an anti-fungal agent to control invasive infections.",
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Molecular cloning and anti-fungal effect of endo-β-1,3-glucanase from Thermotoga maritima. / Woo, Chang Bin; Kang, Hae Na; Lee, Soo Bok.

In: Food Science and Biotechnology, Vol. 23, No. 4, 08.2014, p. 1243-1246.

Research output: Contribution to journalEditorial

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AB - A gene encoding an endo-β-1,3-glucanase from Thermotoga maritima MSB8 (TmβG) was cloned and expressed in Escherichia coli. The purified enzyme produced various β-1,3-glucooligosaccharides from soluble laminarin, and mainly β-1,3-glucooligosaccharides smaller than laminaritetrose from insoluble curdlan. The optimum pH and temperature of the enzyme were 5.0 and 80°C, respectively. TmβG inhibited the growth of Candida albicans, which indicates that the enzyme could potentially be used as an anti-fungal agent to control invasive infections.

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