Molecular cloning and characterization of a major egg antigen in Paragonimus westermani and its use in ELISA for the immunodiagnosis of paragonimiasis

Jae Sik Lee, Jongweon Lee, Sun Hyun Kim, Taisoon Yong

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A recombinant protein of a Paragonimus westermani egg antigen was produced and tested as an antigen for the serologic diagnosis of P. westermani infection. The P. westermani egg antigen gene contains a single open reading frame of 966 base pairs encoding 322 amino acids from 5′ methionine to the 3′ stop codon. The predicted amino acid sequence of this egg antigen was 40, 38, and was 35% identical to heat shock proteins from Schistosoma japonicum, Schistosoma mansoni, and Taenia saginata. The distribution this antigen was investigated in adult worms by indirect immunofluorescence assay, and found to be distributed in eggs and uteri. The specificity and sensitivity of the recombinant antigen were assessed by enzyme-linked immunosorbent assay (ELISA) using sera from patients infected with different parasites, which included 41 patients with paragonimiasis, and negative controls. The diagnostic positive and negative predictive absorbance value was 0.24 and the sensitivity of ELISA using the recombinant antigen was 90.2%, and its specificity 100%. Our results suggest that the developed recombinant major egg antigen-based ELISA offers a highly sensitive and specific assay for the diagnosis of paragonimiasis.

Original languageEnglish
Pages (from-to)677-681
Number of pages5
JournalParasitology Research
Volume100
Issue number4
DOIs
Publication statusPublished - 2007 Mar 1

Fingerprint

Paragonimus westermani
Paragonimiasis
Immunologic Tests
serodiagnosis
Molecular Cloning
Ovum
molecular cloning
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
antigens
Antigens
recombinant antigens
Taenia saginata
Schistosoma japonicum
Schistosoma mansoni
stop codon
assays
heat shock proteins
recombinant proteins
uterus

All Science Journal Classification (ASJC) codes

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

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title = "Molecular cloning and characterization of a major egg antigen in Paragonimus westermani and its use in ELISA for the immunodiagnosis of paragonimiasis",
abstract = "A recombinant protein of a Paragonimus westermani egg antigen was produced and tested as an antigen for the serologic diagnosis of P. westermani infection. The P. westermani egg antigen gene contains a single open reading frame of 966 base pairs encoding 322 amino acids from 5′ methionine to the 3′ stop codon. The predicted amino acid sequence of this egg antigen was 40, 38, and was 35{\%} identical to heat shock proteins from Schistosoma japonicum, Schistosoma mansoni, and Taenia saginata. The distribution this antigen was investigated in adult worms by indirect immunofluorescence assay, and found to be distributed in eggs and uteri. The specificity and sensitivity of the recombinant antigen were assessed by enzyme-linked immunosorbent assay (ELISA) using sera from patients infected with different parasites, which included 41 patients with paragonimiasis, and negative controls. The diagnostic positive and negative predictive absorbance value was 0.24 and the sensitivity of ELISA using the recombinant antigen was 90.2{\%}, and its specificity 100{\%}. Our results suggest that the developed recombinant major egg antigen-based ELISA offers a highly sensitive and specific assay for the diagnosis of paragonimiasis.",
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Molecular cloning and characterization of a major egg antigen in Paragonimus westermani and its use in ELISA for the immunodiagnosis of paragonimiasis. / Lee, Jae Sik; Lee, Jongweon; Kim, Sun Hyun; Yong, Taisoon.

In: Parasitology Research, Vol. 100, No. 4, 01.03.2007, p. 677-681.

Research output: Contribution to journalArticle

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