Abstract
A 2.9 kb DNA fragment encoding s-(2-aminoethyl)-l-cysteine (AEC) resistant aspartokinase of Corynebacteriumglutamicum was cloned on the C.glutamicum/E.coli shuttle vector pECCG117. A recombinant plasmid, designated pAK12, conferred the AEC resistance, the ability to excrete lysine and threonine, and the 3-5 fold increased specific activity of aspartokinase to host strain.
Original language | English |
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Pages (from-to) | 721-726 |
Number of pages | 6 |
Journal | Biotechnology Letters |
Volume | 13 |
Issue number | 10 |
DOIs | |
Publication status | Published - 1991 Oct |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology