Molecular cloning and expression of s-(2-aminoethyl)-L-cysteine resistant aspartokinase gene of Corynebacteriumglutamicum

Jong Kwon Han; Jong Won Oh; Hyune Hwan Lee; Stephen Chung; Hyung Hwan Hyun; Jae Heung Lee

doi:10.1007/BF01088176

Molecular cloning and expression of s-(2-aminoethyl)-L-cysteine resistant aspartokinase gene of Corynebacteriumglutamicum

Jong Kwon Han, Jong Won Oh, Hyune Hwan Lee, Stephen Chung, Hyung Hwan Hyun, Jae Heung Lee

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A 2.9 kb DNA fragment encoding s-(2-aminoethyl)-l-cysteine (AEC) resistant aspartokinase of Corynebacteriumglutamicum was cloned on the C.glutamicum/E.coli shuttle vector pECCG117. A recombinant plasmid, designated pAK12, conferred the AEC resistance, the ability to excrete lysine and threonine, and the 3-5 fold increased specific activity of aspartokinase to host strain.

Original language	English
Pages (from-to)	721-726
Number of pages	6
Journal	Biotechnology Letters
Volume	13
Issue number	10
DOIs	https://doi.org/10.1007/BF01088176
Publication status	Published - 1991 Oct

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/BF01088176

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abstract = "A 2.9 kb DNA fragment encoding s-(2-aminoethyl)-l-cysteine (AEC) resistant aspartokinase of Corynebacteriumglutamicum was cloned on the C.glutamicum/E.coli shuttle vector pECCG117. A recombinant plasmid, designated pAK12, conferred the AEC resistance, the ability to excrete lysine and threonine, and the 3-5 fold increased specific activity of aspartokinase to host strain.",

author = "Han, {Jong Kwon} and Oh, {Jong Won} and Lee, {Hyune Hwan} and Stephen Chung and Hyun, {Hyung Hwan} and Lee, {Jae Heung}",

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doi = "10.1007/BF01088176",

language = "English",

volume = "13",

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journal = "Biotechnology Letters",

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AU - Han, Jong Kwon

AU - Oh, Jong Won

AU - Lee, Hyune Hwan

AU - Chung, Stephen

AU - Hyun, Hyung Hwan

AU - Lee, Jae Heung

PY - 1991/10

Y1 - 1991/10

N2 - A 2.9 kb DNA fragment encoding s-(2-aminoethyl)-l-cysteine (AEC) resistant aspartokinase of Corynebacteriumglutamicum was cloned on the C.glutamicum/E.coli shuttle vector pECCG117. A recombinant plasmid, designated pAK12, conferred the AEC resistance, the ability to excrete lysine and threonine, and the 3-5 fold increased specific activity of aspartokinase to host strain.

AB - A 2.9 kb DNA fragment encoding s-(2-aminoethyl)-l-cysteine (AEC) resistant aspartokinase of Corynebacteriumglutamicum was cloned on the C.glutamicum/E.coli shuttle vector pECCG117. A recombinant plasmid, designated pAK12, conferred the AEC resistance, the ability to excrete lysine and threonine, and the 3-5 fold increased specific activity of aspartokinase to host strain.

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SN - 0141-5492

IS - 10

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Molecular cloning and expression of s-(2-aminoethyl)-L-cysteine resistant aspartokinase gene of Corynebacteriumglutamicum

Abstract

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