Molecular cloning and expression of s-(2-aminoethyl)-L-cysteine resistant aspartokinase gene of Corynebacteriumglutamicum

Jong Kwon Han, Jong-Won Oh, Hyune Hwan Lee, Stephen Chung, Hyung Hwan Hyun, Jae Heung Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A 2.9 kb DNA fragment encoding s-(2-aminoethyl)-l-cysteine (AEC) resistant aspartokinase of Corynebacteriumglutamicum was cloned on the C.glutamicum/E.coli shuttle vector pECCG117. A recombinant plasmid, designated pAK12, conferred the AEC resistance, the ability to excrete lysine and threonine, and the 3-5 fold increased specific activity of aspartokinase to host strain.

Original languageEnglish
Pages (from-to)721-726
Number of pages6
JournalBiotechnology Letters
Volume13
Issue number10
DOIs
Publication statusPublished - 1991 Oct 1

Fingerprint

Aspartate Kinase
Cloning
Molecular Cloning
Escherichia coli
Cysteine
DNA
Genes
Genetic Vectors
Threonine
Lysine
Plasmids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Han, Jong Kwon ; Oh, Jong-Won ; Lee, Hyune Hwan ; Chung, Stephen ; Hyun, Hyung Hwan ; Lee, Jae Heung. / Molecular cloning and expression of s-(2-aminoethyl)-L-cysteine resistant aspartokinase gene of Corynebacteriumglutamicum. In: Biotechnology Letters. 1991 ; Vol. 13, No. 10. pp. 721-726.
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Molecular cloning and expression of s-(2-aminoethyl)-L-cysteine resistant aspartokinase gene of Corynebacteriumglutamicum. / Han, Jong Kwon; Oh, Jong-Won; Lee, Hyune Hwan; Chung, Stephen; Hyun, Hyung Hwan; Lee, Jae Heung.

In: Biotechnology Letters, Vol. 13, No. 10, 01.10.1991, p. 721-726.

Research output: Contribution to journalArticle

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AU - Hyun, Hyung Hwan

AU - Lee, Jae Heung

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