Molecular cloning and expression of the human Δ7-sterol reductase

Fabian F. Moebius, Barbara U. Fitzky, Joon No Lee, Young-Ki Paik, Hartmut Glossmann

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

Inhibitors of the last steps of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development. Their molecular target is the Δ7-sterol reductase (EC 1.3.1.21), suspected to be defective in the Smith- Lemli-Opitz syndrome, a frequent inborn disorder of sterol metabolism. Molecular cloning of the cDNA revealed that the human enzyme is a membrane- bound protein with a predicted molecular mass of 55 kDa and six to nine putative transmembrane segments. The protein is structurally related to plant and yeast sterol reductases. In adults the ubiquitously transcribed mRNA is most abundant in adrenal gland, liver, testis, and brain. The Δ7-sterol reductase is the ultimate enzyme of cholesterol biosynthesis in vertebrates and is absent from yeast. Microsomes from Saccharomyces cerevisiae strains heterologously expressing the human cDNA remove the C7-8 double bond in 7- dehydrocholesterol. The conversion to cholesterol depends an NADPH and is potently inhibited by AY9944 (IC50 0.013 μM), BM15766 (IC50 1.2 μM), and triparanol (IC50 14 μM). Our work paves the way to clarify whether a defect in the Δ7-sterol reductase gene underlies the Smith-Lemli-Opitz syndrome.

Original languageEnglish
Pages (from-to)1899-1902
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number4
DOIs
Publication statusPublished - 1998 Feb 17

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Molecular Cloning
Sterols
trans-1,4-Bis(2-chlorobenzaminomethyl)cyclohexane Dihydrochloride
Oxidoreductases
Smith-Lemli-Opitz Syndrome
Inhibitory Concentration 50
Cholesterol
Triparanol
Complementary DNA
Yeasts
Phytosterols
Brain
Enzymes
Adrenal Glands
Microsomes
NADP
Saccharomyces cerevisiae
Vertebrates
Testis
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

Moebius, Fabian F. ; Fitzky, Barbara U. ; Lee, Joon No ; Paik, Young-Ki ; Glossmann, Hartmut. / Molecular cloning and expression of the human Δ7-sterol reductase. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 4. pp. 1899-1902.
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Molecular cloning and expression of the human Δ7-sterol reductase. / Moebius, Fabian F.; Fitzky, Barbara U.; Lee, Joon No; Paik, Young-Ki; Glossmann, Hartmut.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 4, 17.02.1998, p. 1899-1902.

Research output: Contribution to journalArticle

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