Molecular cloning and functional characterization of an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935

Jang Bin Woo, Hae Na Kang, Eui Jeon Woo, Soo-Bok Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A gene encoding an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935 (SmβG) was characterised by cloning and expressing it in Escherichia coli. The purified enzyme produced β-1,3-glucooligosaccharides, mainly laminaripentaose, from insoluble curdlan. The optimum pH and temperature were 6.0 and 55 C, respectively. SmβG inhibited the growth of Candida albicans, which indicates that this enzyme could be potentially used as an anti-fungal agent to control invasive Candida infections. The results suggest that SmβG may be a useful bioavailable β-1,3-glucanase.

Original languageEnglish
Pages (from-to)184-187
Number of pages4
JournalFood Chemistry
Volume148
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Streptomyces matensis
Candida
Cloning
Streptomyces
Molecular Cloning
molecular cloning
Antifungal agents
curdlan
Gene encoding
candidiasis
Enzymes
enzymes
Candida albicans
Escherichia coli
Organism Cloning
Temperature
Growth
Infection
Genes
temperature

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science

Cite this

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title = "Molecular cloning and functional characterization of an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935",
abstract = "A gene encoding an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935 (SmβG) was characterised by cloning and expressing it in Escherichia coli. The purified enzyme produced β-1,3-glucooligosaccharides, mainly laminaripentaose, from insoluble curdlan. The optimum pH and temperature were 6.0 and 55 C, respectively. SmβG inhibited the growth of Candida albicans, which indicates that this enzyme could be potentially used as an anti-fungal agent to control invasive Candida infections. The results suggest that SmβG may be a useful bioavailable β-1,3-glucanase.",
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Molecular cloning and functional characterization of an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935. / Woo, Jang Bin; Kang, Hae Na; Woo, Eui Jeon; Lee, Soo-Bok.

In: Food Chemistry, Vol. 148, 01.01.2014, p. 184-187.

Research output: Contribution to journalArticle

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