Molecular cloning and identification of a novel oxygenase gene specifically induced during the growth of Rhodococcus sp. strain T104 on limonene

Young Choi Ki, Dockyu Kim, Sung Cheol Koh, Jae Seong So, Jong S. Kim, Eungbin Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Rhodococcus sp. strain T104 is able to utilize both limonene and biphenyl as growth substrates. Furthermore, T104 possesses separate pathways for the degradation of limonene and biphenyl. Previously, we found that a gene(s) involved in limonene degradation was also related to indigo-producing ability. To further corroborate this observation, we have cloned and sequenced a 8,842-bp genomic DNA region with four open reading frames, including one for indole oxygenase, which converts indole to indigo (a blue pigment). The reverse transcription PCR data demonstrated that the identified indole oxygenase gene is specifically induced by limonene, thereby implicating this gene in the degradation of limonene by T104.

Original languageEnglish
Pages (from-to)160-162
Number of pages3
JournalJournal of Microbiology
Volume42
Issue number2
Publication statusPublished - 2004 Jun 1

Fingerprint

Rhodococcus
Oxygenases
Molecular Cloning
Indigo Carmine
Growth
Genes
Open Reading Frames
Reverse Transcription
limonene
Polymerase Chain Reaction
DNA

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Ki, Young Choi ; Kim, Dockyu ; Koh, Sung Cheol ; So, Jae Seong ; Kim, Jong S. ; Kim, Eungbin. / Molecular cloning and identification of a novel oxygenase gene specifically induced during the growth of Rhodococcus sp. strain T104 on limonene. In: Journal of Microbiology. 2004 ; Vol. 42, No. 2. pp. 160-162.
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Molecular cloning and identification of a novel oxygenase gene specifically induced during the growth of Rhodococcus sp. strain T104 on limonene. / Ki, Young Choi; Kim, Dockyu; Koh, Sung Cheol; So, Jae Seong; Kim, Jong S.; Kim, Eungbin.

In: Journal of Microbiology, Vol. 42, No. 2, 01.06.2004, p. 160-162.

Research output: Contribution to journalArticle

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