Molecular cloning and the allergenic characterization of tropomyosin from Tyrophagus putrescentiae

Yong Jeong Kyoung, Haeseok Lee, Sik Lee Jae, Jongweon Lee, In Yong Lee, Han Il Ree, Chein Soo Hong, Tai Soon Yong

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Storage mites have been recognized as a cause of asthma and rhinitis. Studies from several countries have shown that the IgE-mediated allergy to storage mites is of considerable importance, especially in rural populations. This study aimed to identify and characterize new allergens from Tyrophagus putrescentiae. A partial cDNA sequence encoding tropomyosin was isolated from the cDNA library by immunoscreening using anti-mouse IgG1 sera raised against T. putrescentiae whole body extract. The deduced amino acid sequence shares 64-94% identity with previously known allergenic tropomyosins. Its recombinant protein was produced by using a pET 28b expression system and purified by affinity chromatography using Ni-NTA agarose. The IgE reactivities of tropomyosins from T. putrescentiae and Dermatophagoides farinae were compared by enzyme linked immunosorbent assay (ELISA). Recombinant Tyr p 10 showed 12.5% (5/40) IgE-binding reactivity, whereas recombinant Der f 10 showed 25% (10/40) IgE-binding reactivity against the same sera from storage mite-sensitized and house dust mite-sensitized subjects. Both recombinant Tyr p 10 and Der f 10 showed little inhibition of IgE binding to T. putrescentiae crude extract by ELISA. Tropomyosin seems to contribute only a small portion of the cross-reactivity with house dust mites.

Original languageEnglish
Pages (from-to)431-436
Number of pages6
JournalProtein and Peptide Letters
Volume14
Issue number5
DOIs
Publication statusPublished - 2007 May

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry

Fingerprint Dive into the research topics of 'Molecular cloning and the allergenic characterization of tropomyosin from Tyrophagus putrescentiae'. Together they form a unique fingerprint.

Cite this