Molecular epidemiology and resistome analysis of multidrug-resistant ST11 Klebsiella pneumoniae strain containing multiple copies of extended-spectrum β-lactamase genes using whole-genome sequencing

Roshan D. Souza, Naina Adren Pinto, Insik Hwang, Hwang Younjee, Young Lag Cho, Hyunsoo Kim, Dongeun Yong, Jongrak Choi, Kyungwon Lee, Yunsop Chong

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The aim of this work was to investigate the mechanism responsible for multidrug resistance in ST11 Klebsiella pneumoniae YMC 2013/7/B3993 containing multiple copies of ESBL genes using multiple parallel sequencing technology. In-depth analysis of the strain revealed multiple copies of ESBL genes, 2 copies of blaSHV-12 and 1 copy of blaCTX-M-15. Furthermore, 1 copy of blaOXA-9 and 3 copies of blaTEM-1 were found. The insertion of Tn1331 was detected, which consisted of blaOXA-9, blaTEM-1, aac(6')-lb-cr, and aadA1 genes. The acquisition of multiple copies of resistance genes was due to the insertion of transposons in the bacterial genome and plasmid. The genotypic analysis revealed that the isolates belonging to ST11 showed severe resistance phenotypes and greater dissemination potential. To the best of our knowledge, this is the first report demonstrating multiple copies of same ESBL genes in K. pneumoniae ST11 isolate. Furthermore, massive parallel sequencing studies of genetic factors to enhance the fitness of this type strain would be warranted to determine whether ST11 K. pneumoniae can spread the KPC-type gene.

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalNew Microbiologica
Volume40
Issue number1
Publication statusPublished - 2017 Jan

Fingerprint

Molecular Epidemiology
Klebsiella pneumoniae
Genome
Genes
Bacterial Genomes
Multiple Drug Resistance
Plasmids
Technology
Phenotype

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Souza, Roshan D. ; Pinto, Naina Adren ; Hwang, Insik ; Younjee, Hwang ; Cho, Young Lag ; Kim, Hyunsoo ; Yong, Dongeun ; Choi, Jongrak ; Lee, Kyungwon ; Chong, Yunsop. / Molecular epidemiology and resistome analysis of multidrug-resistant ST11 Klebsiella pneumoniae strain containing multiple copies of extended-spectrum β-lactamase genes using whole-genome sequencing. In: New Microbiologica. 2017 ; Vol. 40, No. 1. pp. 38-44.
@article{d925d59728954d308c36deaeb0d985f3,
title = "Molecular epidemiology and resistome analysis of multidrug-resistant ST11 Klebsiella pneumoniae strain containing multiple copies of extended-spectrum β-lactamase genes using whole-genome sequencing",
abstract = "The aim of this work was to investigate the mechanism responsible for multidrug resistance in ST11 Klebsiella pneumoniae YMC 2013/7/B3993 containing multiple copies of ESBL genes using multiple parallel sequencing technology. In-depth analysis of the strain revealed multiple copies of ESBL genes, 2 copies of blaSHV-12 and 1 copy of blaCTX-M-15. Furthermore, 1 copy of blaOXA-9 and 3 copies of blaTEM-1 were found. The insertion of Tn1331 was detected, which consisted of blaOXA-9, blaTEM-1, aac(6')-lb-cr, and aadA1 genes. The acquisition of multiple copies of resistance genes was due to the insertion of transposons in the bacterial genome and plasmid. The genotypic analysis revealed that the isolates belonging to ST11 showed severe resistance phenotypes and greater dissemination potential. To the best of our knowledge, this is the first report demonstrating multiple copies of same ESBL genes in K. pneumoniae ST11 isolate. Furthermore, massive parallel sequencing studies of genetic factors to enhance the fitness of this type strain would be warranted to determine whether ST11 K. pneumoniae can spread the KPC-type gene.",
author = "Souza, {Roshan D.} and Pinto, {Naina Adren} and Insik Hwang and Hwang Younjee and Cho, {Young Lag} and Hyunsoo Kim and Dongeun Yong and Jongrak Choi and Kyungwon Lee and Yunsop Chong",
year = "2017",
month = "1",
language = "English",
volume = "40",
pages = "38--44",
journal = "New Microbiologica",
issn = "1121-7138",
publisher = "Luigi Ponzio e figlio Editori",
number = "1",

}

Molecular epidemiology and resistome analysis of multidrug-resistant ST11 Klebsiella pneumoniae strain containing multiple copies of extended-spectrum β-lactamase genes using whole-genome sequencing. / Souza, Roshan D.; Pinto, Naina Adren; Hwang, Insik; Younjee, Hwang; Cho, Young Lag; Kim, Hyunsoo; Yong, Dongeun; Choi, Jongrak; Lee, Kyungwon; Chong, Yunsop.

In: New Microbiologica, Vol. 40, No. 1, 01.2017, p. 38-44.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Molecular epidemiology and resistome analysis of multidrug-resistant ST11 Klebsiella pneumoniae strain containing multiple copies of extended-spectrum β-lactamase genes using whole-genome sequencing

AU - Souza, Roshan D.

AU - Pinto, Naina Adren

AU - Hwang, Insik

AU - Younjee, Hwang

AU - Cho, Young Lag

AU - Kim, Hyunsoo

AU - Yong, Dongeun

AU - Choi, Jongrak

AU - Lee, Kyungwon

AU - Chong, Yunsop

PY - 2017/1

Y1 - 2017/1

N2 - The aim of this work was to investigate the mechanism responsible for multidrug resistance in ST11 Klebsiella pneumoniae YMC 2013/7/B3993 containing multiple copies of ESBL genes using multiple parallel sequencing technology. In-depth analysis of the strain revealed multiple copies of ESBL genes, 2 copies of blaSHV-12 and 1 copy of blaCTX-M-15. Furthermore, 1 copy of blaOXA-9 and 3 copies of blaTEM-1 were found. The insertion of Tn1331 was detected, which consisted of blaOXA-9, blaTEM-1, aac(6')-lb-cr, and aadA1 genes. The acquisition of multiple copies of resistance genes was due to the insertion of transposons in the bacterial genome and plasmid. The genotypic analysis revealed that the isolates belonging to ST11 showed severe resistance phenotypes and greater dissemination potential. To the best of our knowledge, this is the first report demonstrating multiple copies of same ESBL genes in K. pneumoniae ST11 isolate. Furthermore, massive parallel sequencing studies of genetic factors to enhance the fitness of this type strain would be warranted to determine whether ST11 K. pneumoniae can spread the KPC-type gene.

AB - The aim of this work was to investigate the mechanism responsible for multidrug resistance in ST11 Klebsiella pneumoniae YMC 2013/7/B3993 containing multiple copies of ESBL genes using multiple parallel sequencing technology. In-depth analysis of the strain revealed multiple copies of ESBL genes, 2 copies of blaSHV-12 and 1 copy of blaCTX-M-15. Furthermore, 1 copy of blaOXA-9 and 3 copies of blaTEM-1 were found. The insertion of Tn1331 was detected, which consisted of blaOXA-9, blaTEM-1, aac(6')-lb-cr, and aadA1 genes. The acquisition of multiple copies of resistance genes was due to the insertion of transposons in the bacterial genome and plasmid. The genotypic analysis revealed that the isolates belonging to ST11 showed severe resistance phenotypes and greater dissemination potential. To the best of our knowledge, this is the first report demonstrating multiple copies of same ESBL genes in K. pneumoniae ST11 isolate. Furthermore, massive parallel sequencing studies of genetic factors to enhance the fitness of this type strain would be warranted to determine whether ST11 K. pneumoniae can spread the KPC-type gene.

UR - http://www.scopus.com/inward/record.url?scp=85018784995&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018784995&partnerID=8YFLogxK

M3 - Article

C2 - 28072891

AN - SCOPUS:85018784995

VL - 40

SP - 38

EP - 44

JO - New Microbiologica

JF - New Microbiologica

SN - 1121-7138

IS - 1

ER -