Molecular epidemiology of community-associated antimicrobial-resistant Staphylococcus aureus in Seoul, Korea (2003)

Pervasiveness of multidrug-resistant SCCmec type II methicillin-resistant S. aureus

Hye Yoon Jeong, Jung Eun Lee, Bo Kyung Choi, Kyung Won Seo, Seung Hee Park, Young Lim Kim, Kyoung Min Baek, Kyungwon Lee, Dong Kwon Rhee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

There is an extremely high incidence of antimicrobial resistance of the clinical isolates of Staphylococcus aureus in Korea. This study carried out a molecular investigation to determine the prevalence of the community-associated antimicrobial-resistant S. aureus and methicillin-resistant S. aureus (MRSA). The percentage resistance from the nasal swabs of healthy volunteers in 2003 in Seoul is as follows: penicillin (91%), erythromycin (EM, 14%), gentamicin (GM, 9.3%), tetracycline (TE, 8.2%), cephalothin (4%), oxacillin (OX, MRSA; 3.8%), clindamycin (CC, 2.6%), ciprofloxacin (CIP, 0.8%), and sulfamethoxazole/ trimethoprim (0.6%). The community-associated MRSA (C-MRSA) strains were examined by pulsed-field gel electrophoresis (PFGE) analysis of the SmaI macro-fragments, multilocus sequence typing (MLST), and staphylococcal cassette chromosome mec (SCCmec) typing using the PCR analysis. The Korean C-MRSA isolates were clustered into three distinct groups. One PFGE group containing the C-MRSA strains showed resistance to CC, EM, and GM, a high level (32-96 μg/ml) of resistance to methicillin, sequence type 5 (ST5), and SCCmec type II, which is the most common hospital associated-MRSA (H-MRSA) isolated in Korea. These results highlight the heterogeneous genetic background of the C-MRSA as well as the pervasiveness of the H-MRSA isolates in this community.

Original languageEnglish
Pages (from-to)178-185
Number of pages8
JournalMicrobial Drug Resistance
Volume13
Issue number3
DOIs
Publication statusPublished - 2007 Sep 1

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Molecular Epidemiology
Korea
Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus
Chromosomes
Pulsed Field Gel Electrophoresis
Cephalothin
Multilocus Sequence Typing
Oxacillin
Methicillin Resistance
Clindamycin
Seoul
Sulfamethoxazole Drug Combination Trimethoprim
Erythromycin
Ciprofloxacin
Gentamicins
Tetracycline
Nose
Penicillins
Healthy Volunteers

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Pharmacology
  • Microbiology (medical)

Cite this

Jeong, Hye Yoon ; Lee, Jung Eun ; Choi, Bo Kyung ; Seo, Kyung Won ; Park, Seung Hee ; Kim, Young Lim ; Baek, Kyoung Min ; Lee, Kyungwon ; Rhee, Dong Kwon. / Molecular epidemiology of community-associated antimicrobial-resistant Staphylococcus aureus in Seoul, Korea (2003) : Pervasiveness of multidrug-resistant SCCmec type II methicillin-resistant S. aureus. In: Microbial Drug Resistance. 2007 ; Vol. 13, No. 3. pp. 178-185.
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abstract = "There is an extremely high incidence of antimicrobial resistance of the clinical isolates of Staphylococcus aureus in Korea. This study carried out a molecular investigation to determine the prevalence of the community-associated antimicrobial-resistant S. aureus and methicillin-resistant S. aureus (MRSA). The percentage resistance from the nasal swabs of healthy volunteers in 2003 in Seoul is as follows: penicillin (91{\%}), erythromycin (EM, 14{\%}), gentamicin (GM, 9.3{\%}), tetracycline (TE, 8.2{\%}), cephalothin (4{\%}), oxacillin (OX, MRSA; 3.8{\%}), clindamycin (CC, 2.6{\%}), ciprofloxacin (CIP, 0.8{\%}), and sulfamethoxazole/ trimethoprim (0.6{\%}). The community-associated MRSA (C-MRSA) strains were examined by pulsed-field gel electrophoresis (PFGE) analysis of the SmaI macro-fragments, multilocus sequence typing (MLST), and staphylococcal cassette chromosome mec (SCCmec) typing using the PCR analysis. The Korean C-MRSA isolates were clustered into three distinct groups. One PFGE group containing the C-MRSA strains showed resistance to CC, EM, and GM, a high level (32-96 μg/ml) of resistance to methicillin, sequence type 5 (ST5), and SCCmec type II, which is the most common hospital associated-MRSA (H-MRSA) isolated in Korea. These results highlight the heterogeneous genetic background of the C-MRSA as well as the pervasiveness of the H-MRSA isolates in this community.",
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Molecular epidemiology of community-associated antimicrobial-resistant Staphylococcus aureus in Seoul, Korea (2003) : Pervasiveness of multidrug-resistant SCCmec type II methicillin-resistant S. aureus. / Jeong, Hye Yoon; Lee, Jung Eun; Choi, Bo Kyung; Seo, Kyung Won; Park, Seung Hee; Kim, Young Lim; Baek, Kyoung Min; Lee, Kyungwon; Rhee, Dong Kwon.

In: Microbial Drug Resistance, Vol. 13, No. 3, 01.09.2007, p. 178-185.

Research output: Contribution to journalArticle

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