Molecular identification of proline-rich protein genes induced during root formation in grape (Vitis vinifera L.) stem cuttings

P. Thomas, M. M. Lee, John Schiefelbein

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Vegetative reproduction relies on the initiation of new plant organs in response to environmental changes. The rapid formation of roots, and ultimately whole plants, from stem cuttings of grape (Vitis vinifera L.) provides a useful system to investigate the physiological and molecular basis of organ initiation during vegetative reproduction. In the present study the differential RNA display technique was employed to identify two genes, VvPRP1 and VvPRP2, that are induced in stem cuttings of grape during rooting. Each of these genes encodes a distinct type of proline-rich protein that is related to different groups of putative cell wall proteins, and their expression is rapidly induced in stem segments within 6 h after severing. Further, each gene's transcript becomes most concentrated in the basal portion of the stem segment in the region of new root formation. Induction of these genes is not significantly enhanced by indole-3-acetic acid (IAA) treatment, and the expression of the VvPRP1 gene, but not the VvPRP2 gene, is wound-inducible. These results suggest that these VvPRP genes play an important role in the initiation of new roots on grape stem cuttings, perhaps by altering the cell wall mechanical properties to enable root emergence.

Original languageEnglish
Pages (from-to)1497-1504
Number of pages8
JournalPlant, Cell and Environment
Volume26
Issue number9
DOIs
Publication statusPublished - 2003 Sep 1

Fingerprint

Vitis
Proline
Vitis vinifera
proline
grapes
stems
Genes
Proteins
genes
proteins
Cell Wall
Reproduction
cell walls
Plant Stems
gene induction
plant damage
plant organs
acid treatment
indole acetic acid
mechanical properties

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

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abstract = "Vegetative reproduction relies on the initiation of new plant organs in response to environmental changes. The rapid formation of roots, and ultimately whole plants, from stem cuttings of grape (Vitis vinifera L.) provides a useful system to investigate the physiological and molecular basis of organ initiation during vegetative reproduction. In the present study the differential RNA display technique was employed to identify two genes, VvPRP1 and VvPRP2, that are induced in stem cuttings of grape during rooting. Each of these genes encodes a distinct type of proline-rich protein that is related to different groups of putative cell wall proteins, and their expression is rapidly induced in stem segments within 6 h after severing. Further, each gene's transcript becomes most concentrated in the basal portion of the stem segment in the region of new root formation. Induction of these genes is not significantly enhanced by indole-3-acetic acid (IAA) treatment, and the expression of the VvPRP1 gene, but not the VvPRP2 gene, is wound-inducible. These results suggest that these VvPRP genes play an important role in the initiation of new roots on grape stem cuttings, perhaps by altering the cell wall mechanical properties to enable root emergence.",
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Molecular identification of proline-rich protein genes induced during root formation in grape (Vitis vinifera L.) stem cuttings. / Thomas, P.; Lee, M. M.; Schiefelbein, John.

In: Plant, Cell and Environment, Vol. 26, No. 9, 01.09.2003, p. 1497-1504.

Research output: Contribution to journalArticle

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