Molecular overlap with optical near-fields based on plasmonic nanolithography for ultrasensitive label-free detection by light-matter colocalization

Kiheung Kim, Wonju Lee, Kyungwha Chung, Hongki Lee, Taehwang Son, Youngjin Oh, Yun Feng Xiao, Dong Ha Kim, Donghyun Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In this work, we investigate the detection sensitivity of surface plasmon resonance (SPR) biosensors by engineering spatial distribution of electromagnetic near-fields for colocalization with molecular distribution. The light-matter colocalization was based on plasmonic nanolithography, the concept of which was confirmed by detecting streptavidin biotin interactions on triangular nanoaperture arrays after the structure of the aperture arrays was optimized for colocalization efficiency. The colocalization was shown to amplify optical signature significantly and thereby to achieve detection on the order of 100 streptavidin molecules with a binding capacity below 1 fg/mm2, an enhancement by more than three orders of magnitude over conventional SPR detection.

Original languageEnglish
Pages (from-to)89-98
Number of pages10
JournalBiosensors and Bioelectronics
Volume96
DOIs
Publication statusPublished - 2017 Oct 15

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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