Molecular proteomics imaging of tumor interfaces by mass spectrometry

Suki Kang, Hyo Sup Shim, Jong Sik Lee, Dong Su Kim, Hak Yong Kim, Seong Hyun Hong, Pan Soo Kim, Joo Heon Yoon, Nam Hoon Cho

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The specific molecular profiles of ovarian cancer interface zones (IZ), the region between tumors and normal tissues, were evaluated using a new method involving matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS). We analyzed three ovarian serous carcinomas using MALDI-IMS. Principal component analysis (PCA) was used to evaluate the quality of tissue spatial features based on MALDI-IMS, and for analysis of large data sets of MALDI-IMS. Twodimensional gel electrophoresis and fluorescence microscopy were used to verify interface-specific proteins. Unique profiles were identified for the tumors, the normal zone, and the IZ. Through MALDI analysis, two interfacespecific proteins, plastin 2 and peroxiredoxin 1 (PRDX 1), were identified as differentially regulated between zones. Fluorescence microscopy revealed high expression levels of plastin 2 and PRDX 1 along the IZ of ovarian tumors. This comparative proteomics study using tissue MALDIIMS suggested that the IZ is different from the adjacent tumor and normal zones, and that plastin 2 and PRDX 1 may be interface markers specific to ovarian tumors.

Original languageEnglish
Pages (from-to)1157-1164
Number of pages8
JournalJournal of Proteome Research
Volume9
Issue number2
DOIs
Publication statusPublished - 2010 Feb 5

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

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    Kang, S., Shim, H. S., Lee, J. S., Kim, D. S., Kim, H. Y., Hong, S. H., Kim, P. S., Yoon, J. H., & Cho, N. H. (2010). Molecular proteomics imaging of tumor interfaces by mass spectrometry. Journal of Proteome Research, 9(2), 1157-1164. https://doi.org/10.1021/pr900666q