Molecular recognition of proteolytic activity in metastatic cancer cells using fluorogenic gold nanoprobes

Yoochan Hong, Minhee Ku, Dan Heo, Seungyeon Hwang, Eugene Lee, Joseph Park, Jihye Choi, Hyeon Jung Lee, Miran Seo, Eunjig Lee, Jong In Yook, Seungjoo Haam, yongmin Huh, Dae Sung Yoon, Jinsuck Suh, Jaemoon Yang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We describe the development of biomarker-sensitive nanoprobes based on nanoparticle surface energy transfer (NSET) effect that enabling recognition of the expression of membrane type-1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells and its proteolytic activity simultaneously. First of all, we confirmed invasiveness of cancer cell lines (HT1080 and MCF7) via migration and invasion assay. We also prepared gold nanoparticle (GNP) acts as a quencher for fluorescein isothiocyanate (FITC). This FITC is conjugated in end-terminal of activatable fluorogenic peptide (ActFP). The ActFP attach to surface of GNP (GNP-ActFP) for a targeting moiety and proteolytic activity ligand toward MT1-MMP. The GNP-ActFP can generate fluorescence signal when ActFP is cleaved by proteolytic activity after targeting toward MT1-MMP. In order to study specificity for MT1-MMP, GNP-ActFP is treated to HT1080 and MCF7 cells, and then, we determine the in vitro targeting potential and fluorogenic activity of GNP-ActFP for MT1-MMP via fluorescence multi-reader. We also confirmed fluorogenic activity of GNP-ActFP via confocal microscopic imaging, and finally, endocytosis of GNP-ActFP is observed via cellular transmission electron microscopic imaging.

Original languageEnglish
Pages (from-to)171-178
Number of pages8
JournalBiosensors and Bioelectronics
Volume57
DOIs
Publication statusPublished - 2014 Jul 15

Fingerprint

Nanoprobes
Molecular recognition
Gold
Nanoparticles
Peptides
Matrix Metalloproteinase 14
Cells
Neoplasms
Fluorescein
Fluorescence
Imaging techniques
Energy Transfer
MCF-7 Cells
Biomarkers
Endocytosis
Interfacial energy
Energy transfer
Assays
Ligands
Electrons

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Hong, Yoochan ; Ku, Minhee ; Heo, Dan ; Hwang, Seungyeon ; Lee, Eugene ; Park, Joseph ; Choi, Jihye ; Jung Lee, Hyeon ; Seo, Miran ; Lee, Eunjig ; In Yook, Jong ; Haam, Seungjoo ; Huh, yongmin ; Sung Yoon, Dae ; Suh, Jinsuck ; Yang, Jaemoon. / Molecular recognition of proteolytic activity in metastatic cancer cells using fluorogenic gold nanoprobes. In: Biosensors and Bioelectronics. 2014 ; Vol. 57. pp. 171-178.
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abstract = "We describe the development of biomarker-sensitive nanoprobes based on nanoparticle surface energy transfer (NSET) effect that enabling recognition of the expression of membrane type-1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells and its proteolytic activity simultaneously. First of all, we confirmed invasiveness of cancer cell lines (HT1080 and MCF7) via migration and invasion assay. We also prepared gold nanoparticle (GNP) acts as a quencher for fluorescein isothiocyanate (FITC). This FITC is conjugated in end-terminal of activatable fluorogenic peptide (ActFP). The ActFP attach to surface of GNP (GNP-ActFP) for a targeting moiety and proteolytic activity ligand toward MT1-MMP. The GNP-ActFP can generate fluorescence signal when ActFP is cleaved by proteolytic activity after targeting toward MT1-MMP. In order to study specificity for MT1-MMP, GNP-ActFP is treated to HT1080 and MCF7 cells, and then, we determine the in vitro targeting potential and fluorogenic activity of GNP-ActFP for MT1-MMP via fluorescence multi-reader. We also confirmed fluorogenic activity of GNP-ActFP via confocal microscopic imaging, and finally, endocytosis of GNP-ActFP is observed via cellular transmission electron microscopic imaging.",
author = "Yoochan Hong and Minhee Ku and Dan Heo and Seungyeon Hwang and Eugene Lee and Joseph Park and Jihye Choi and {Jung Lee}, Hyeon and Miran Seo and Eunjig Lee and {In Yook}, Jong and Seungjoo Haam and yongmin Huh and {Sung Yoon}, Dae and Jinsuck Suh and Jaemoon Yang",
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Hong, Y, Ku, M, Heo, D, Hwang, S, Lee, E, Park, J, Choi, J, Jung Lee, H, Seo, M, Lee, E, In Yook, J, Haam, S, Huh, Y, Sung Yoon, D, Suh, J & Yang, J 2014, 'Molecular recognition of proteolytic activity in metastatic cancer cells using fluorogenic gold nanoprobes', Biosensors and Bioelectronics, vol. 57, pp. 171-178. https://doi.org/10.1016/j.bios.2014.02.011

Molecular recognition of proteolytic activity in metastatic cancer cells using fluorogenic gold nanoprobes. / Hong, Yoochan; Ku, Minhee; Heo, Dan; Hwang, Seungyeon; Lee, Eugene; Park, Joseph; Choi, Jihye; Jung Lee, Hyeon; Seo, Miran; Lee, Eunjig; In Yook, Jong; Haam, Seungjoo; Huh, yongmin; Sung Yoon, Dae; Suh, Jinsuck; Yang, Jaemoon.

In: Biosensors and Bioelectronics, Vol. 57, 15.07.2014, p. 171-178.

Research output: Contribution to journalArticle

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T1 - Molecular recognition of proteolytic activity in metastatic cancer cells using fluorogenic gold nanoprobes

AU - Hong, Yoochan

AU - Ku, Minhee

AU - Heo, Dan

AU - Hwang, Seungyeon

AU - Lee, Eugene

AU - Park, Joseph

AU - Choi, Jihye

AU - Jung Lee, Hyeon

AU - Seo, Miran

AU - Lee, Eunjig

AU - In Yook, Jong

AU - Haam, Seungjoo

AU - Huh, yongmin

AU - Sung Yoon, Dae

AU - Suh, Jinsuck

AU - Yang, Jaemoon

PY - 2014/7/15

Y1 - 2014/7/15

N2 - We describe the development of biomarker-sensitive nanoprobes based on nanoparticle surface energy transfer (NSET) effect that enabling recognition of the expression of membrane type-1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells and its proteolytic activity simultaneously. First of all, we confirmed invasiveness of cancer cell lines (HT1080 and MCF7) via migration and invasion assay. We also prepared gold nanoparticle (GNP) acts as a quencher for fluorescein isothiocyanate (FITC). This FITC is conjugated in end-terminal of activatable fluorogenic peptide (ActFP). The ActFP attach to surface of GNP (GNP-ActFP) for a targeting moiety and proteolytic activity ligand toward MT1-MMP. The GNP-ActFP can generate fluorescence signal when ActFP is cleaved by proteolytic activity after targeting toward MT1-MMP. In order to study specificity for MT1-MMP, GNP-ActFP is treated to HT1080 and MCF7 cells, and then, we determine the in vitro targeting potential and fluorogenic activity of GNP-ActFP for MT1-MMP via fluorescence multi-reader. We also confirmed fluorogenic activity of GNP-ActFP via confocal microscopic imaging, and finally, endocytosis of GNP-ActFP is observed via cellular transmission electron microscopic imaging.

AB - We describe the development of biomarker-sensitive nanoprobes based on nanoparticle surface energy transfer (NSET) effect that enabling recognition of the expression of membrane type-1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells and its proteolytic activity simultaneously. First of all, we confirmed invasiveness of cancer cell lines (HT1080 and MCF7) via migration and invasion assay. We also prepared gold nanoparticle (GNP) acts as a quencher for fluorescein isothiocyanate (FITC). This FITC is conjugated in end-terminal of activatable fluorogenic peptide (ActFP). The ActFP attach to surface of GNP (GNP-ActFP) for a targeting moiety and proteolytic activity ligand toward MT1-MMP. The GNP-ActFP can generate fluorescence signal when ActFP is cleaved by proteolytic activity after targeting toward MT1-MMP. In order to study specificity for MT1-MMP, GNP-ActFP is treated to HT1080 and MCF7 cells, and then, we determine the in vitro targeting potential and fluorogenic activity of GNP-ActFP for MT1-MMP via fluorescence multi-reader. We also confirmed fluorogenic activity of GNP-ActFP via confocal microscopic imaging, and finally, endocytosis of GNP-ActFP is observed via cellular transmission electron microscopic imaging.

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