Molecular sensing for biomarkers of invasive cancer cells using localized surface plasmon resonance

Yoochan Hong, Minhee Ku, Jinsuck Suh, yongmin Huh, Dae Sung Yoon, Jaemoon Yang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we describe the development of cancer biomarker-sensitive nanobiosensor based on localized surface plasmon resonance (LSPR) that enabling recognition for proteolytic activity of membrane type 1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells. First of all, we prepared biomarker-detectable substrate based on gold nanorods (GNRs) using nanoparticle adsorption method. The sensitivity of sensing chip was confirmed using various solvents that having different refractive indexes. Subsequently, MT1-MMP-specific cleavable peptide was conjugated onto surface of GNRs and molecular sensing about proteolytic activity was conducted using MT1-MMP and cell lysates. Collectively, we developed biomarker detectable sensor, which allows for the effective detection of proteolytic activity about MT1-MMP extracted from invasive cancer cells.

Original languageEnglish
Title of host publicationNano-Bio Sensing, Imaging, and Spectroscopy
Volume8879
DOIs
Publication statusPublished - 2013 Sep 17
EventSPIE Nano-Bio Sensing, Imaging, and Spectroscopy - Jeju, Korea, Republic of
Duration: 2013 Feb 202013 Feb 23

Other

OtherSPIE Nano-Bio Sensing, Imaging, and Spectroscopy
CountryKorea, Republic of
CityJeju
Period13/2/2013/2/23

Fingerprint

Localized Surface Plasmon Resonance
Matrix Metalloproteinase 14
biomarkers
Biomarkers
Surface plasmon resonance
surface plasmon resonance
Cancer
Sensing
Membrane
cancer
Cells
membranes
Membranes
Nanorods
Cell
matrices
Gold
nanorods
gold
Tumor Biomarkers

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Hong, Y., Ku, M., Suh, J., Huh, Y., Yoon, D. S., & Yang, J. (2013). Molecular sensing for biomarkers of invasive cancer cells using localized surface plasmon resonance. In Nano-Bio Sensing, Imaging, and Spectroscopy (Vol. 8879). [88790Z] https://doi.org/10.1117/12.2018326
Hong, Yoochan ; Ku, Minhee ; Suh, Jinsuck ; Huh, yongmin ; Yoon, Dae Sung ; Yang, Jaemoon. / Molecular sensing for biomarkers of invasive cancer cells using localized surface plasmon resonance. Nano-Bio Sensing, Imaging, and Spectroscopy. Vol. 8879 2013.
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Hong, Y, Ku, M, Suh, J, Huh, Y, Yoon, DS & Yang, J 2013, Molecular sensing for biomarkers of invasive cancer cells using localized surface plasmon resonance. in Nano-Bio Sensing, Imaging, and Spectroscopy. vol. 8879, 88790Z, SPIE Nano-Bio Sensing, Imaging, and Spectroscopy, Jeju, Korea, Republic of, 13/2/20. https://doi.org/10.1117/12.2018326

Molecular sensing for biomarkers of invasive cancer cells using localized surface plasmon resonance. / Hong, Yoochan; Ku, Minhee; Suh, Jinsuck; Huh, yongmin; Yoon, Dae Sung; Yang, Jaemoon.

Nano-Bio Sensing, Imaging, and Spectroscopy. Vol. 8879 2013. 88790Z.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Hong Y, Ku M, Suh J, Huh Y, Yoon DS, Yang J. Molecular sensing for biomarkers of invasive cancer cells using localized surface plasmon resonance. In Nano-Bio Sensing, Imaging, and Spectroscopy. Vol. 8879. 2013. 88790Z https://doi.org/10.1117/12.2018326