Molecular weight and structure characterization of sodium hyaluronate and its gamma radiation degradation products by flow field-flow fractionation and on-line multiangle light scattering

Da Young Shin, Euijin Hwang, Il Hwan Cho, Myeong Hee Moon

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A combined flow field-flow fractionation (FlFFF)/multiangle light scattering (MALS)/differential refractive index (DRI) detection method has been utilized for the size fractionation and characterization of ultrahigh molecular weight (MW) sodium hyaluronate (NaHA) samples. Separation of broad MW NaHA polymers was carried out by a frit inlet asymmetrical FlFFF channel employed with a linear field programming method followed by the on-line monitoring of light scattering at multiple angles for the calculation of MW and for the study of the conformation of NaHA samples. NaHA samples examined were: (1) two different viscosity fractions of NaHA obtained by a refinement process and (2) NaHA products of gamma radiation degradation. While the NaHA samples of two different viscosity fractions exhibited clearly different MW distributions and similar conformation, the radiation degraded NaHA samples showed a clear difference in both MW distribution and polymer structure.

Original languageEnglish
Pages (from-to)270-275
Number of pages6
JournalJournal of Chromatography A
Volume1160
Issue number1-2
DOIs
Publication statusPublished - 2007 Aug 10

Fingerprint

Hyaluronic Acid
Fractionation
Gamma rays
Light scattering
Molecular structure
Flow fields
Molecular weight
Molecular weight distribution
Degradation
Conformations
Polymers
Viscosity
Inlet flow
Refractive index
Radiation
Monitoring

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

@article{3a5a2016723b4ce7bcb712d0d5f7a2e8,
title = "Molecular weight and structure characterization of sodium hyaluronate and its gamma radiation degradation products by flow field-flow fractionation and on-line multiangle light scattering",
abstract = "A combined flow field-flow fractionation (FlFFF)/multiangle light scattering (MALS)/differential refractive index (DRI) detection method has been utilized for the size fractionation and characterization of ultrahigh molecular weight (MW) sodium hyaluronate (NaHA) samples. Separation of broad MW NaHA polymers was carried out by a frit inlet asymmetrical FlFFF channel employed with a linear field programming method followed by the on-line monitoring of light scattering at multiple angles for the calculation of MW and for the study of the conformation of NaHA samples. NaHA samples examined were: (1) two different viscosity fractions of NaHA obtained by a refinement process and (2) NaHA products of gamma radiation degradation. While the NaHA samples of two different viscosity fractions exhibited clearly different MW distributions and similar conformation, the radiation degraded NaHA samples showed a clear difference in both MW distribution and polymer structure.",
author = "Shin, {Da Young} and Euijin Hwang and Cho, {Il Hwan} and Moon, {Myeong Hee}",
year = "2007",
month = "8",
day = "10",
doi = "10.1016/j.chroma.2007.05.047",
language = "English",
volume = "1160",
pages = "270--275",
journal = "Journal of Chromatography A",
issn = "0021-9673",
number = "1-2",

}

TY - JOUR

T1 - Molecular weight and structure characterization of sodium hyaluronate and its gamma radiation degradation products by flow field-flow fractionation and on-line multiangle light scattering

AU - Shin, Da Young

AU - Hwang, Euijin

AU - Cho, Il Hwan

AU - Moon, Myeong Hee

PY - 2007/8/10

Y1 - 2007/8/10

N2 - A combined flow field-flow fractionation (FlFFF)/multiangle light scattering (MALS)/differential refractive index (DRI) detection method has been utilized for the size fractionation and characterization of ultrahigh molecular weight (MW) sodium hyaluronate (NaHA) samples. Separation of broad MW NaHA polymers was carried out by a frit inlet asymmetrical FlFFF channel employed with a linear field programming method followed by the on-line monitoring of light scattering at multiple angles for the calculation of MW and for the study of the conformation of NaHA samples. NaHA samples examined were: (1) two different viscosity fractions of NaHA obtained by a refinement process and (2) NaHA products of gamma radiation degradation. While the NaHA samples of two different viscosity fractions exhibited clearly different MW distributions and similar conformation, the radiation degraded NaHA samples showed a clear difference in both MW distribution and polymer structure.

AB - A combined flow field-flow fractionation (FlFFF)/multiangle light scattering (MALS)/differential refractive index (DRI) detection method has been utilized for the size fractionation and characterization of ultrahigh molecular weight (MW) sodium hyaluronate (NaHA) samples. Separation of broad MW NaHA polymers was carried out by a frit inlet asymmetrical FlFFF channel employed with a linear field programming method followed by the on-line monitoring of light scattering at multiple angles for the calculation of MW and for the study of the conformation of NaHA samples. NaHA samples examined were: (1) two different viscosity fractions of NaHA obtained by a refinement process and (2) NaHA products of gamma radiation degradation. While the NaHA samples of two different viscosity fractions exhibited clearly different MW distributions and similar conformation, the radiation degraded NaHA samples showed a clear difference in both MW distribution and polymer structure.

UR - http://www.scopus.com/inward/record.url?scp=34447504970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447504970&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2007.05.047

DO - 10.1016/j.chroma.2007.05.047

M3 - Article

C2 - 17537445

AN - SCOPUS:34447504970

VL - 1160

SP - 270

EP - 275

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2

ER -