Monitoring expression of bphC gene from Ralstonia eutropha H850 induced by plant terpenes in soil

Kyung Ja Jung, Byung Hyuk Kim, Eungbin Kim, Jae Seong So, Sung Cheol Koh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A PCB degrader, Ralstonia eutropha H850 was shown to induce bphC gene encoding 2,3-dihydroxybiphenyl-1,2-dioxygenase in a carvone-amended pure culture in our previous study (Park et al., 1999). The present study was carried out to examine how plant terpenes, as natural substrates, would cause an expression of a PCB degradative gene in soil that was amended with terpenes. The population of Ralstonia eutropha H850 was maintained at least around 108 (CFU/g fresh soil) in the soil amended with carvone or limonene in the presence of succinate as a growth substrate at 50 th day. The gene expression was monitored by RT-PCR using total RNA directly extracted from each soil and bphC gene primers. The bphC gene expression of the seeded strain H850 was observed in the soil amended with biphenyl (4 days) but not with succinate, carvone and limonene. These results indicate that terpenes widely distributed in nature could be a potential inducing substrate for effective PCB biodegration in the soil but their bioavailability and specific induction behavior should be taken into account before PCB bioremediation implementation.

Original languageEnglish
Pages (from-to)340-343
Number of pages4
JournalJournal of Microbiology
Volume40
Issue number4
Publication statusPublished - 2002 Dec 1

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

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