Monitoring the status of T-cell activation in a microfluidic system

Joo Young Park, Hyun Ok Kim, Kyun Do Kim, Sung Kyu Kim, Sang Kyou Lee, Hyungil Jung

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Leukocyte adhesion to the endothelium through surface molecules such as E-selectin and intercellular adhesion molecule-1 (ICAM-1) is a critical cellular event reflecting the physiological status of both cell types. Here we present a microfluidic system that can not only easily monitor the interaction between leukocytes and endothelial cells under physiological conditions, but also screen drug candidates for potential modulation of this interaction. Shear stress, which is an important factor for the binding of activated T cells to tumor necrosis factor-alpha (TNF-α)-treated human umbilical vein endothelial cells (HUVECs), was easily controlled by adjusting the flow rate in the microfluidic system. Whole blood of patients with systemic lupus erythematosus (SLE) who have auto-reactive T cells were infused into the activated HUVECs which subsequently showed a higher level of binding compared to a control blood sample from a person without SLE. When these autoreactive T cells were treated with immunosuppressors tacrolimus and cyclosporin A, the binding of the T cells to HUVECs was dramatically decreased. Therefore, this microfluidic system is capable of differentiating the physiological status of T cells or endothelial cells representing different disease conditions, as well as being useful for the identification of novel reagents that modulate the functions of leukocytes or endothelial cells.

Original languageEnglish
Pages (from-to)2831-2836
Number of pages6
JournalAnalyst
Volume136
Issue number13
DOIs
Publication statusPublished - 2011 Jul 7

Fingerprint

Microfluidics
T-cells
Endothelial cells
adhesion
blood
Chemical activation
T-Lymphocytes
Human Umbilical Vein Endothelial Cells
Monitoring
monitoring
Leukocytes
tumor
Endothelial Cells
shear stress
drug
Systemic Lupus Erythematosus
Blood
Adhesion
E-Selectin
Tacrolimus

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Park, Joo Young ; Kim, Hyun Ok ; Kim, Kyun Do ; Kim, Sung Kyu ; Lee, Sang Kyou ; Jung, Hyungil. / Monitoring the status of T-cell activation in a microfluidic system. In: Analyst. 2011 ; Vol. 136, No. 13. pp. 2831-2836.
@article{a69a6a515211448380b5b3d54dd872a4,
title = "Monitoring the status of T-cell activation in a microfluidic system",
abstract = "Leukocyte adhesion to the endothelium through surface molecules such as E-selectin and intercellular adhesion molecule-1 (ICAM-1) is a critical cellular event reflecting the physiological status of both cell types. Here we present a microfluidic system that can not only easily monitor the interaction between leukocytes and endothelial cells under physiological conditions, but also screen drug candidates for potential modulation of this interaction. Shear stress, which is an important factor for the binding of activated T cells to tumor necrosis factor-alpha (TNF-α)-treated human umbilical vein endothelial cells (HUVECs), was easily controlled by adjusting the flow rate in the microfluidic system. Whole blood of patients with systemic lupus erythematosus (SLE) who have auto-reactive T cells were infused into the activated HUVECs which subsequently showed a higher level of binding compared to a control blood sample from a person without SLE. When these autoreactive T cells were treated with immunosuppressors tacrolimus and cyclosporin A, the binding of the T cells to HUVECs was dramatically decreased. Therefore, this microfluidic system is capable of differentiating the physiological status of T cells or endothelial cells representing different disease conditions, as well as being useful for the identification of novel reagents that modulate the functions of leukocytes or endothelial cells.",
author = "Park, {Joo Young} and Kim, {Hyun Ok} and Kim, {Kyun Do} and Kim, {Sung Kyu} and Lee, {Sang Kyou} and Hyungil Jung",
year = "2011",
month = "7",
day = "7",
doi = "10.1039/c1an15038c",
language = "English",
volume = "136",
pages = "2831--2836",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "13",

}

Monitoring the status of T-cell activation in a microfluidic system. / Park, Joo Young; Kim, Hyun Ok; Kim, Kyun Do; Kim, Sung Kyu; Lee, Sang Kyou; Jung, Hyungil.

In: Analyst, Vol. 136, No. 13, 07.07.2011, p. 2831-2836.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Monitoring the status of T-cell activation in a microfluidic system

AU - Park, Joo Young

AU - Kim, Hyun Ok

AU - Kim, Kyun Do

AU - Kim, Sung Kyu

AU - Lee, Sang Kyou

AU - Jung, Hyungil

PY - 2011/7/7

Y1 - 2011/7/7

N2 - Leukocyte adhesion to the endothelium through surface molecules such as E-selectin and intercellular adhesion molecule-1 (ICAM-1) is a critical cellular event reflecting the physiological status of both cell types. Here we present a microfluidic system that can not only easily monitor the interaction between leukocytes and endothelial cells under physiological conditions, but also screen drug candidates for potential modulation of this interaction. Shear stress, which is an important factor for the binding of activated T cells to tumor necrosis factor-alpha (TNF-α)-treated human umbilical vein endothelial cells (HUVECs), was easily controlled by adjusting the flow rate in the microfluidic system. Whole blood of patients with systemic lupus erythematosus (SLE) who have auto-reactive T cells were infused into the activated HUVECs which subsequently showed a higher level of binding compared to a control blood sample from a person without SLE. When these autoreactive T cells were treated with immunosuppressors tacrolimus and cyclosporin A, the binding of the T cells to HUVECs was dramatically decreased. Therefore, this microfluidic system is capable of differentiating the physiological status of T cells or endothelial cells representing different disease conditions, as well as being useful for the identification of novel reagents that modulate the functions of leukocytes or endothelial cells.

AB - Leukocyte adhesion to the endothelium through surface molecules such as E-selectin and intercellular adhesion molecule-1 (ICAM-1) is a critical cellular event reflecting the physiological status of both cell types. Here we present a microfluidic system that can not only easily monitor the interaction between leukocytes and endothelial cells under physiological conditions, but also screen drug candidates for potential modulation of this interaction. Shear stress, which is an important factor for the binding of activated T cells to tumor necrosis factor-alpha (TNF-α)-treated human umbilical vein endothelial cells (HUVECs), was easily controlled by adjusting the flow rate in the microfluidic system. Whole blood of patients with systemic lupus erythematosus (SLE) who have auto-reactive T cells were infused into the activated HUVECs which subsequently showed a higher level of binding compared to a control blood sample from a person without SLE. When these autoreactive T cells were treated with immunosuppressors tacrolimus and cyclosporin A, the binding of the T cells to HUVECs was dramatically decreased. Therefore, this microfluidic system is capable of differentiating the physiological status of T cells or endothelial cells representing different disease conditions, as well as being useful for the identification of novel reagents that modulate the functions of leukocytes or endothelial cells.

UR - http://www.scopus.com/inward/record.url?scp=79959971780&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959971780&partnerID=8YFLogxK

U2 - 10.1039/c1an15038c

DO - 10.1039/c1an15038c

M3 - Article

VL - 136

SP - 2831

EP - 2836

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 13

ER -