Monocyte enrichment from leukapheresis products by using the Elutra cell separator

Sinyoung Kim, Hyun Ok Kim, Eun Jung Baek, Youjeong Choi, Han Soo Kim, Min Geul Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

BACKGROUND: Dendritic cells (DCs), used in clinical trials for cancer immunotherapy, require processing on an expanded scale to conform to current good manufacturing practice guidelines. This study evaluated a large-scale monocyte enrichment procedure with a commercially available cell separator (Elutra, Gambro BCT) and analyzed the capacity of enriched monocytes to differentiate into DCs. STUDY DESIGN AND METHODS: Mononuclear cells were collected in two patients with malignant melanoma and seven healthy donors by leukapheresis. Continuous-counterflow elutriation with the Elutra was performed to enrich and purify monocytes from leukapheresis products. Purity and recovery of enriched monocytes were analyzed by flow cytometry. DCs were generated from the elutriated monocytes and characterized by phenotypic surface marker and stimulatory capacity in an allogeneic mixed lymphocyte reaction. RESULTS: In the leukapheresis products, the total MNC count was 7.3 × 109 ± 0.7 × 109 and the mean percentage of CD14+ monocytes was 16.5 ± 3.8 percent, which increased to 68.9 ± 7.4 percent after elutriation with the Elutra. The mean monocyte recovery was 94.3 percent. Elutriated monocytes were successfully cultured into phenotypically and functionally mature DCs. CONCLUSION: These results indicate that the Elutra cell separator allows for fast and easy enrichment of monocytes within a closed system. Furthermore, these monocytes can be differentiated into functionally mature DCs. Compared to plastic adherence and immunomagnetic selection methods, the elutriation procedure is inexpensive, efficient, and very effective.

Original languageEnglish
Pages (from-to)2290-2296
Number of pages7
JournalTransfusion
Volume47
Issue number12
DOIs
Publication statusPublished - 2007 Dec 1

Fingerprint

Leukapheresis
Monocytes
Dendritic Cells
Mixed Lymphocyte Culture Test
Practice Guidelines
Immunotherapy
Plastics
Melanoma
Flow Cytometry
Tissue Donors
Clinical Trials

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

Kim, Sinyoung ; Kim, Hyun Ok ; Baek, Eun Jung ; Choi, Youjeong ; Kim, Han Soo ; Lee, Min Geul. / Monocyte enrichment from leukapheresis products by using the Elutra cell separator. In: Transfusion. 2007 ; Vol. 47, No. 12. pp. 2290-2296.
@article{7da55e4f788e403f80431376f4e40e9f,
title = "Monocyte enrichment from leukapheresis products by using the Elutra cell separator",
abstract = "BACKGROUND: Dendritic cells (DCs), used in clinical trials for cancer immunotherapy, require processing on an expanded scale to conform to current good manufacturing practice guidelines. This study evaluated a large-scale monocyte enrichment procedure with a commercially available cell separator (Elutra, Gambro BCT) and analyzed the capacity of enriched monocytes to differentiate into DCs. STUDY DESIGN AND METHODS: Mononuclear cells were collected in two patients with malignant melanoma and seven healthy donors by leukapheresis. Continuous-counterflow elutriation with the Elutra was performed to enrich and purify monocytes from leukapheresis products. Purity and recovery of enriched monocytes were analyzed by flow cytometry. DCs were generated from the elutriated monocytes and characterized by phenotypic surface marker and stimulatory capacity in an allogeneic mixed lymphocyte reaction. RESULTS: In the leukapheresis products, the total MNC count was 7.3 × 109 ± 0.7 × 109 and the mean percentage of CD14+ monocytes was 16.5 ± 3.8 percent, which increased to 68.9 ± 7.4 percent after elutriation with the Elutra. The mean monocyte recovery was 94.3 percent. Elutriated monocytes were successfully cultured into phenotypically and functionally mature DCs. CONCLUSION: These results indicate that the Elutra cell separator allows for fast and easy enrichment of monocytes within a closed system. Furthermore, these monocytes can be differentiated into functionally mature DCs. Compared to plastic adherence and immunomagnetic selection methods, the elutriation procedure is inexpensive, efficient, and very effective.",
author = "Sinyoung Kim and Kim, {Hyun Ok} and Baek, {Eun Jung} and Youjeong Choi and Kim, {Han Soo} and Lee, {Min Geul}",
year = "2007",
month = "12",
day = "1",
doi = "10.1111/j.1537-2995.2007.01470.x",
language = "English",
volume = "47",
pages = "2290--2296",
journal = "Transfusion",
issn = "0041-1132",
publisher = "Wiley-Blackwell",
number = "12",

}

Monocyte enrichment from leukapheresis products by using the Elutra cell separator. / Kim, Sinyoung; Kim, Hyun Ok; Baek, Eun Jung; Choi, Youjeong; Kim, Han Soo; Lee, Min Geul.

In: Transfusion, Vol. 47, No. 12, 01.12.2007, p. 2290-2296.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Monocyte enrichment from leukapheresis products by using the Elutra cell separator

AU - Kim, Sinyoung

AU - Kim, Hyun Ok

AU - Baek, Eun Jung

AU - Choi, Youjeong

AU - Kim, Han Soo

AU - Lee, Min Geul

PY - 2007/12/1

Y1 - 2007/12/1

N2 - BACKGROUND: Dendritic cells (DCs), used in clinical trials for cancer immunotherapy, require processing on an expanded scale to conform to current good manufacturing practice guidelines. This study evaluated a large-scale monocyte enrichment procedure with a commercially available cell separator (Elutra, Gambro BCT) and analyzed the capacity of enriched monocytes to differentiate into DCs. STUDY DESIGN AND METHODS: Mononuclear cells were collected in two patients with malignant melanoma and seven healthy donors by leukapheresis. Continuous-counterflow elutriation with the Elutra was performed to enrich and purify monocytes from leukapheresis products. Purity and recovery of enriched monocytes were analyzed by flow cytometry. DCs were generated from the elutriated monocytes and characterized by phenotypic surface marker and stimulatory capacity in an allogeneic mixed lymphocyte reaction. RESULTS: In the leukapheresis products, the total MNC count was 7.3 × 109 ± 0.7 × 109 and the mean percentage of CD14+ monocytes was 16.5 ± 3.8 percent, which increased to 68.9 ± 7.4 percent after elutriation with the Elutra. The mean monocyte recovery was 94.3 percent. Elutriated monocytes were successfully cultured into phenotypically and functionally mature DCs. CONCLUSION: These results indicate that the Elutra cell separator allows for fast and easy enrichment of monocytes within a closed system. Furthermore, these monocytes can be differentiated into functionally mature DCs. Compared to plastic adherence and immunomagnetic selection methods, the elutriation procedure is inexpensive, efficient, and very effective.

AB - BACKGROUND: Dendritic cells (DCs), used in clinical trials for cancer immunotherapy, require processing on an expanded scale to conform to current good manufacturing practice guidelines. This study evaluated a large-scale monocyte enrichment procedure with a commercially available cell separator (Elutra, Gambro BCT) and analyzed the capacity of enriched monocytes to differentiate into DCs. STUDY DESIGN AND METHODS: Mononuclear cells were collected in two patients with malignant melanoma and seven healthy donors by leukapheresis. Continuous-counterflow elutriation with the Elutra was performed to enrich and purify monocytes from leukapheresis products. Purity and recovery of enriched monocytes were analyzed by flow cytometry. DCs were generated from the elutriated monocytes and characterized by phenotypic surface marker and stimulatory capacity in an allogeneic mixed lymphocyte reaction. RESULTS: In the leukapheresis products, the total MNC count was 7.3 × 109 ± 0.7 × 109 and the mean percentage of CD14+ monocytes was 16.5 ± 3.8 percent, which increased to 68.9 ± 7.4 percent after elutriation with the Elutra. The mean monocyte recovery was 94.3 percent. Elutriated monocytes were successfully cultured into phenotypically and functionally mature DCs. CONCLUSION: These results indicate that the Elutra cell separator allows for fast and easy enrichment of monocytes within a closed system. Furthermore, these monocytes can be differentiated into functionally mature DCs. Compared to plastic adherence and immunomagnetic selection methods, the elutriation procedure is inexpensive, efficient, and very effective.

UR - http://www.scopus.com/inward/record.url?scp=36349015333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36349015333&partnerID=8YFLogxK

U2 - 10.1111/j.1537-2995.2007.01470.x

DO - 10.1111/j.1537-2995.2007.01470.x

M3 - Article

VL - 47

SP - 2290

EP - 2296

JO - Transfusion

JF - Transfusion

SN - 0041-1132

IS - 12

ER -