Our study investigated the histogenesis and biological significance of spindle cell carcinoma (SpCC) by comparing with moderately-well-differentiated squamous cell carcinoma (SCC) cell lines. Snail mRNA expression was readily detectable in the SpCC cell line, while E-cadherin was undetectable. SpCC cells showed lower proliferative and invasive activities than two other SCC cell lines. Culturing under air-liquid interface conditions promoted squamous cell differentiation, whereas fibroblastic differentiation after submerged culture with collagen, suggesting that the microenvironment may be a regulating factor of spindle cell differentiation as well as Snail expression and spindle cell change may not always entail the invasive behavior.
Bibliographical noteFunding Information:
This research has been supported by the Korean Ministry of Health and Welfare Grant No. HMP-00-B-20507-0177 and by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD), Basic research promotion fund (KRF-2005-005-05901). The authors express sincere gratitude to Dr. Tae Jung Kwon, National Institute of Scientific Investigation for expert interpretation of ultrastructural pictures, and to Dr. Darren Williams, Yonsei University for critical reading the manuscript.
All Science Journal Classification (ASJC) codes
- Cancer Research