Morphogenic potentials of D2, D3, and D4 dopamine receptors revealed in transfected neuronal cell lines

Barbara C. Swarzenski, Lei Tang, Young Jun Oh, Karen L. O'Malley, Richard D. Todd

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Molecular cloning studies have defined a family of dopamine D2-like receptors (D2, D3, and D4), which are the products of separate genes. Our previous work has shown that stimulation of dopamine D2-like receptors in cultures of fetal cortical neurons increases the extension and branching of neurites. To determine which D2-like receptors possess morphogenic potentials, a clonal mesencephalic cell line (MN9D) was transfected with D2, D3, or D4 receptor subtypes and treated with quinpirole, an agonist of D2- like receptors, and changes in morphological characteristics were quantitated. Stimulation of D2 receptors increased the number and branching of neurites with little effect on neurite extension; stimulation of D3 and D4 receptors increased the branching and extension of neurites. Similar results were found for primary mesencephalic cultures stimulated with quinpirole. These results suggest that the known D2-like receptors have specific developmental roles in regulating neuronal morphogenesis of dopaminergic pathways.

Original languageEnglish
Pages (from-to)649-653
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number2
DOIs
Publication statusPublished - 1994 Jan 18

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Dopamine D4 Receptors
Dopamine D3 Receptors
Dopamine D2 Receptors
Neurites
Quinpirole
Cell Line
Molecular Cloning
Morphogenesis
Neurons
Genes

All Science Journal Classification (ASJC) codes

  • General

Cite this

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abstract = "Molecular cloning studies have defined a family of dopamine D2-like receptors (D2, D3, and D4), which are the products of separate genes. Our previous work has shown that stimulation of dopamine D2-like receptors in cultures of fetal cortical neurons increases the extension and branching of neurites. To determine which D2-like receptors possess morphogenic potentials, a clonal mesencephalic cell line (MN9D) was transfected with D2, D3, or D4 receptor subtypes and treated with quinpirole, an agonist of D2- like receptors, and changes in morphological characteristics were quantitated. Stimulation of D2 receptors increased the number and branching of neurites with little effect on neurite extension; stimulation of D3 and D4 receptors increased the branching and extension of neurites. Similar results were found for primary mesencephalic cultures stimulated with quinpirole. These results suggest that the known D2-like receptors have specific developmental roles in regulating neuronal morphogenesis of dopaminergic pathways.",
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Morphogenic potentials of D2, D3, and D4 dopamine receptors revealed in transfected neuronal cell lines. / Swarzenski, Barbara C.; Tang, Lei; Oh, Young Jun; O'Malley, Karen L.; Todd, Richard D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 2, 18.01.1994, p. 649-653.

Research output: Contribution to journalArticle

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