Morphological and molecular changes associated with Pitchfork during mouse palate development

Chengri Jin, Jong Min Lee, Qinghuang Tang, Liwen Li, Min Jung Lee, Hyoung Seon Baik, Han Sung Jung

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mammalian palate development is regulated by complex processes. Many cellular and molecular events, such as cell proliferation, apoptosis, cell migration and the epithelial mesenchymal transition, regulate proper palate development and some abnormalities in palate development lead to cleft palate. Various developmental disorders, such as cleft palate and disorders of the lung, kidney and heart, are known to be associated with ciliary defects. Pitchfork, a mouse embryonic node gene, is associated with ciliary targeting complexes located at the basal body during primary cilia disassembly. To determine the function of Pitchfork during palate development, we examine Pitchfork expression patterns and morphological changes in the developing secondary palate after Pitchfork over-expression. From embryonic day 12.5 (E12.5) to E13.5 in mice, Pitchfork was highly expressed in the developing mouse secondary palate. Morphological differences were observed in vitro in cultured palates in the Pitchfork over-expression group compared with the control group. Pitchfork over-expression induced primary cilia disassembly during palate development. Sonic hedgehog and Patched1 expression levels and palatine rugae morphology were altered in the over-expressed Pitchfork group during palate development. Thus, the proper expression levels of Pitchfork might play a pivotal role in normal secondary palate morphogenesis.

Original languageEnglish
Pages (from-to)385-393
Number of pages9
JournalCell and Tissue Research
Volume358
Issue number2
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Palate
Cilia
Cleft Palate
Basal Bodies
Epithelial-Mesenchymal Transition
Hedgehogs
Morphogenesis
Cell Movement
Cell Proliferation
Apoptosis
Kidney
Lung
Control Groups

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Jin, Chengri ; Lee, Jong Min ; Tang, Qinghuang ; Li, Liwen ; Lee, Min Jung ; Baik, Hyoung Seon ; Jung, Han Sung. / Morphological and molecular changes associated with Pitchfork during mouse palate development. In: Cell and Tissue Research. 2014 ; Vol. 358, No. 2. pp. 385-393.
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Morphological and molecular changes associated with Pitchfork during mouse palate development. / Jin, Chengri; Lee, Jong Min; Tang, Qinghuang; Li, Liwen; Lee, Min Jung; Baik, Hyoung Seon; Jung, Han Sung.

In: Cell and Tissue Research, Vol. 358, No. 2, 01.01.2014, p. 385-393.

Research output: Contribution to journalArticle

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