Morphological evidence of basal keratinocyte migration during the re-epithelialization process

Akihiro Hosoya, Jong Min Lee, Sung Won Cho, Ji Youn Kim, Naoshi Shinozaki, Takahiko Shibahara, Masaki Shimono, Hansung Jung

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The regeneration of wounded stratified epithelium is accomplished via the migration of keratinocytes from the margins of the wound. However, the process of keratinocyte migration on the wound surface and the role of epithelial stem cells during re-epithelialization remain to be elucidated. Therefore, we administered BrdU to embryonic mice and generated epithelial defects on the buccal mucosa of these mice at two weeks after birth, using CO 2 laser irradiation, with which we removed the entire thickness of the epithelium. In the unwounded epithelium, cytokeratin 14, p63, and BrdU were localized within the basal layer of the epithelium, but the majority of cells within the regenerated epithelium were immunopositive for these proteins. PCNA-negative and BrdU-positive basal keratinocytes, which evidence a slow cell cycle, were localized solely within the basal layer of the unwound epithelium facing the tips of dermal papillae. After laser irradiation, these basal keratinocytes facing the tips of the papillae evidenced positive immunoreactivity for PCNA, in addition to BrdU. These results indicate that epithelial stem cells of oral mucosa may be localized in the basal layer of the epithelium facing the tips of dermal papillae, and may migrate laterally with other basal keratinocytes in response to external stimuli.

Original languageEnglish
Pages (from-to)1165-1175
Number of pages11
JournalHistochemistry and cell biology
Volume130
Issue number6
DOIs
Publication statusPublished - 2008 Dec 1

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Re-Epithelialization
Keratinocytes
Epithelium
Bromodeoxyuridine
Proliferating Cell Nuclear Antigen
Mouth Mucosa
Lasers
Stem Cells
Epithelial Cells
Keratin-14
Skin
Wounds and Injuries
Carbon Monoxide
Regeneration
Cell Cycle
Parturition

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Hosoya, Akihiro ; Lee, Jong Min ; Cho, Sung Won ; Kim, Ji Youn ; Shinozaki, Naoshi ; Shibahara, Takahiko ; Shimono, Masaki ; Jung, Hansung. / Morphological evidence of basal keratinocyte migration during the re-epithelialization process. In: Histochemistry and cell biology. 2008 ; Vol. 130, No. 6. pp. 1165-1175.
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Hosoya, A, Lee, JM, Cho, SW, Kim, JY, Shinozaki, N, Shibahara, T, Shimono, M & Jung, H 2008, 'Morphological evidence of basal keratinocyte migration during the re-epithelialization process', Histochemistry and cell biology, vol. 130, no. 6, pp. 1165-1175. https://doi.org/10.1007/s00418-008-0499-3

Morphological evidence of basal keratinocyte migration during the re-epithelialization process. / Hosoya, Akihiro; Lee, Jong Min; Cho, Sung Won; Kim, Ji Youn; Shinozaki, Naoshi; Shibahara, Takahiko; Shimono, Masaki; Jung, Hansung.

In: Histochemistry and cell biology, Vol. 130, No. 6, 01.12.2008, p. 1165-1175.

Research output: Contribution to journalArticle

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AU - Jung, Hansung

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