Abstract
The regeneration of wounded stratified epithelium is accomplished via the migration of keratinocytes from the margins of the wound. However, the process of keratinocyte migration on the wound surface and the role of epithelial stem cells during re-epithelialization remain to be elucidated. Therefore, we administered BrdU to embryonic mice and generated epithelial defects on the buccal mucosa of these mice at two weeks after birth, using CO2 laser irradiation, with which we removed the entire thickness of the epithelium. In the unwounded epithelium, cytokeratin 14, p63, and BrdU were localized within the basal layer of the epithelium, but the majority of cells within the regenerated epithelium were immunopositive for these proteins. PCNA-negative and BrdU-positive basal keratinocytes, which evidence a slow cell cycle, were localized solely within the basal layer of the unwound epithelium facing the tips of dermal papillae. After laser irradiation, these basal keratinocytes facing the tips of the papillae evidenced positive immunoreactivity for PCNA, in addition to BrdU. These results indicate that epithelial stem cells of oral mucosa may be localized in the basal layer of the epithelium facing the tips of dermal papillae, and may migrate laterally with other basal keratinocytes in response to external stimuli.
| Original language | English |
|---|---|
| Pages (from-to) | 1165-1175 |
| Number of pages | 11 |
| Journal | Histochemistry and cell biology |
| Volume | 130 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2008 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Histology
- Molecular Biology
- Medical Laboratory Technology
- Cell Biology
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Morphological evidence of basal keratinocyte migration during the re-epithelialization process. / Hosoya, Akihiro; Lee, Jong Min; Cho, Sung Won; Kim, Ji Youn; Shinozaki, Naoshi; Shibahara, Takahiko; Shimono, Masaki; Jung, Han Sung.
In: Histochemistry and cell biology, Vol. 130, No. 6, 01.12.2008, p. 1165-1175.Research output: Contribution to journal › Article
TY - JOUR
T1 - Morphological evidence of basal keratinocyte migration during the re-epithelialization process
AU - Hosoya, Akihiro
AU - Lee, Jong Min
AU - Cho, Sung Won
AU - Kim, Ji Youn
AU - Shinozaki, Naoshi
AU - Shibahara, Takahiko
AU - Shimono, Masaki
AU - Jung, Han Sung
PY - 2008/12/1
Y1 - 2008/12/1
N2 - The regeneration of wounded stratified epithelium is accomplished via the migration of keratinocytes from the margins of the wound. However, the process of keratinocyte migration on the wound surface and the role of epithelial stem cells during re-epithelialization remain to be elucidated. Therefore, we administered BrdU to embryonic mice and generated epithelial defects on the buccal mucosa of these mice at two weeks after birth, using CO2 laser irradiation, with which we removed the entire thickness of the epithelium. In the unwounded epithelium, cytokeratin 14, p63, and BrdU were localized within the basal layer of the epithelium, but the majority of cells within the regenerated epithelium were immunopositive for these proteins. PCNA-negative and BrdU-positive basal keratinocytes, which evidence a slow cell cycle, were localized solely within the basal layer of the unwound epithelium facing the tips of dermal papillae. After laser irradiation, these basal keratinocytes facing the tips of the papillae evidenced positive immunoreactivity for PCNA, in addition to BrdU. These results indicate that epithelial stem cells of oral mucosa may be localized in the basal layer of the epithelium facing the tips of dermal papillae, and may migrate laterally with other basal keratinocytes in response to external stimuli.
AB - The regeneration of wounded stratified epithelium is accomplished via the migration of keratinocytes from the margins of the wound. However, the process of keratinocyte migration on the wound surface and the role of epithelial stem cells during re-epithelialization remain to be elucidated. Therefore, we administered BrdU to embryonic mice and generated epithelial defects on the buccal mucosa of these mice at two weeks after birth, using CO2 laser irradiation, with which we removed the entire thickness of the epithelium. In the unwounded epithelium, cytokeratin 14, p63, and BrdU were localized within the basal layer of the epithelium, but the majority of cells within the regenerated epithelium were immunopositive for these proteins. PCNA-negative and BrdU-positive basal keratinocytes, which evidence a slow cell cycle, were localized solely within the basal layer of the unwound epithelium facing the tips of dermal papillae. After laser irradiation, these basal keratinocytes facing the tips of the papillae evidenced positive immunoreactivity for PCNA, in addition to BrdU. These results indicate that epithelial stem cells of oral mucosa may be localized in the basal layer of the epithelium facing the tips of dermal papillae, and may migrate laterally with other basal keratinocytes in response to external stimuli.
UR - http://www.scopus.com/inward/record.url?scp=56749095722&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=56749095722&partnerID=8YFLogxK
U2 - 10.1007/s00418-008-0499-3
DO - 10.1007/s00418-008-0499-3
M3 - Article
C2 - 18773217
AN - SCOPUS:56749095722
VL - 130
SP - 1165
EP - 1175
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
SN - 0948-6143
IS - 6
ER -