Morphometric evaluation of oesophageal wall in patients with nutcracker oesophagus and ineffective oesophageal motility

H. S. Kim, HyoJin Park, J. H. Lim, S. H. Choi, C. Park, S. I. Lee, J. L. Conklin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The pathogenesis of nutcracker oesophagus (NE) and ineffective oesophageal motility (IEM) is unclear. Damage to the enteric nervous system or smooth muscle can cause oesophageal dysmotility. We tested the hypothesis that NE and IEM are associated with abnormal muscular or neural constituents of the oesophageal wall. Oesophageal manometry was performed in patients prior to total gastrectomy for gastric cancer. The oesophageal manometries were categorized as normal (n = 7), NE (n = 13), or IEM (n = 5). Histologic examination of oesophageal tissue obtained during surgery was performed after haematoxylin and eosin (H&E) and trichrome staining. Oesophageal innervation was examined after immunostaining for protein gene product-9.5 (PGP-9.5), choline acetyltransferase (ChAT) and neuronal nitric oxide synthase (nNOS). There were no significant differences in inner circular smooth muscle thickness or degree of fibrosis among the three groups. Severe muscle fibre loss was found in four of five patients with IEM. The density of PGP-9.5-reactive neural structures was not different among the three groups. The density of ChAT immunostaining in the myenteric plexus (MP) was significantly greater in patients with NE (P < 0.05) and the density of nNOS immunostaining in the circular muscle (CM) was significantly greater in IEM patients (P < 0.05). The ChAT/nNOS ratio in both MP and CM was significantly greater in NE patients. NE may result from an imbalance between the excitatory and inhibitory innervation of the oesophagus, because more than normal numbers of ChAT-positive myenteric neurones are seen in NE. Myopathy and/or increased number of nNOS neurones may contribute to the hypocontractile motor activity of IEM.

Original languageEnglish
Pages (from-to)869-876
Number of pages8
JournalNeurogastroenterology and Motility
Volume20
Issue number8
DOIs
Publication statusPublished - 2008 Aug 1

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Esophageal Motility Disorders
Nitric Oxide Synthase Type I
Choline O-Acetyltransferase
Myenteric Plexus
Manometry
Muscles
Smooth Muscle
Enteric Nervous System
Neurons
Muscular Diseases
Hematoxylin
Gastrectomy
Eosine Yellowish-(YS)
Esophagus
Stomach Neoplasms
Proteins
Motor Activity
Fibrosis
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology

Cite this

Kim, H. S. ; Park, HyoJin ; Lim, J. H. ; Choi, S. H. ; Park, C. ; Lee, S. I. ; Conklin, J. L. / Morphometric evaluation of oesophageal wall in patients with nutcracker oesophagus and ineffective oesophageal motility. In: Neurogastroenterology and Motility. 2008 ; Vol. 20, No. 8. pp. 869-876.
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Morphometric evaluation of oesophageal wall in patients with nutcracker oesophagus and ineffective oesophageal motility. / Kim, H. S.; Park, HyoJin; Lim, J. H.; Choi, S. H.; Park, C.; Lee, S. I.; Conklin, J. L.

In: Neurogastroenterology and Motility, Vol. 20, No. 8, 01.08.2008, p. 869-876.

Research output: Contribution to journalArticle

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AU - Kim, H. S.

AU - Park, HyoJin

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AB - The pathogenesis of nutcracker oesophagus (NE) and ineffective oesophageal motility (IEM) is unclear. Damage to the enteric nervous system or smooth muscle can cause oesophageal dysmotility. We tested the hypothesis that NE and IEM are associated with abnormal muscular or neural constituents of the oesophageal wall. Oesophageal manometry was performed in patients prior to total gastrectomy for gastric cancer. The oesophageal manometries were categorized as normal (n = 7), NE (n = 13), or IEM (n = 5). Histologic examination of oesophageal tissue obtained during surgery was performed after haematoxylin and eosin (H&E) and trichrome staining. Oesophageal innervation was examined after immunostaining for protein gene product-9.5 (PGP-9.5), choline acetyltransferase (ChAT) and neuronal nitric oxide synthase (nNOS). There were no significant differences in inner circular smooth muscle thickness or degree of fibrosis among the three groups. Severe muscle fibre loss was found in four of five patients with IEM. The density of PGP-9.5-reactive neural structures was not different among the three groups. The density of ChAT immunostaining in the myenteric plexus (MP) was significantly greater in patients with NE (P < 0.05) and the density of nNOS immunostaining in the circular muscle (CM) was significantly greater in IEM patients (P < 0.05). The ChAT/nNOS ratio in both MP and CM was significantly greater in NE patients. NE may result from an imbalance between the excitatory and inhibitory innervation of the oesophagus, because more than normal numbers of ChAT-positive myenteric neurones are seen in NE. Myopathy and/or increased number of nNOS neurones may contribute to the hypocontractile motor activity of IEM.

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