Mountain ginseng extract exhibits anti-lung cancer activity by inhibiting the nuclear translocation of NF-κB

Jeong Won Hwang, Jung Han Oh, Hwa Seung Yoo, Yeon Weol Lee, Chong Kwan Cho, Ki Rok Kwon, Jung Ho Yoon, Junsoo Park, Song Her, Zee Won Lee, Ik Soon Jang, Jong Soon Choi

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Abstract

Administration of mountain ginseng (MG) extract can restore advanced cancer to a normal state. To elucidate the mechanism by which MG extract prevents the progression of lung cancer, the processes of proliferation and death of lung cancer cells (A549) were examined after treatment with MG extract. Butanol-extracted MG (BX-MG) showed a high inhibitory effect (IC50 = 2 mg/ml) by attenuating proliferation and inducing apoptosis in lung cancer cells. By HPLC-UV analysis of BX-MG, ginsenosides, Rb1 was identified as the most abundant ginsenoside, followed by Rg1, Re, Rc and Rb2. BX-MG induced caspase-3 dependent apoptosis by inhibiting NF-κB. In addition, BX-MG activated p53 and p21, resulting in the attenuated proliferation of A549 cells. Reduced activity of the NF-κB promoter and increased activity of the p53 promoter indicate that BX-MG regulates apoptosis at the level of transcription in lung cancer cells. Furthermore, BX-MG blocked the nuclear translocation of RelA and the associated reduction in surviving. These results suggest that BX-MG inhibits lung cancer cell growth by activating tumor suppressors and inhibiting nuclear translocation of NF-κB.

Original languageEnglish
Pages (from-to)187-202
Number of pages16
JournalAmerican Journal of Chinese Medicine
Volume40
Issue number1
DOIs
Publication statusPublished - 2012 Feb 10

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All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine

Cite this

Hwang, J. W., Oh, J. H., Yoo, H. S., Lee, Y. W., Cho, C. K., Kwon, K. R., Yoon, J. H., Park, J., Her, S., Lee, Z. W., Jang, I. S., & Choi, J. S. (2012). Mountain ginseng extract exhibits anti-lung cancer activity by inhibiting the nuclear translocation of NF-κB. American Journal of Chinese Medicine, 40(1), 187-202. https://doi.org/10.1142/S0192415X12500152