Administration of mountain ginseng (MG) extract can restore advanced cancer to a normal state. To elucidate the mechanism by which MG extract prevents the progression of lung cancer, the processes of proliferation and death of lung cancer cells (A549) were examined after treatment with MG extract. Butanol-extracted MG (BX-MG) showed a high inhibitory effect (IC50 = 2 mg/ml) by attenuating proliferation and inducing apoptosis in lung cancer cells. By HPLC-UV analysis of BX-MG, ginsenosides, Rb1 was identified as the most abundant ginsenoside, followed by Rg1, Re, Rc and Rb2. BX-MG induced caspase-3 dependent apoptosis by inhibiting NF-κB. In addition, BX-MG activated p53 and p21, resulting in the attenuated proliferation of A549 cells. Reduced activity of the NF-κB promoter and increased activity of the p53 promoter indicate that BX-MG regulates apoptosis at the level of transcription in lung cancer cells. Furthermore, BX-MG blocked the nuclear translocation of RelA and the associated reduction in surviving. These results suggest that BX-MG inhibits lung cancer cell growth by activating tumor suppressors and inhibiting nuclear translocation of NF-κB.
Bibliographical noteFunding Information:
This work was financially supported by a Korea Basic Science Institute NAP grant (T3178B) to J.S. Choi and by the Mid-career Researcher Program through an NRF grant funded by the MEST (No. No RO1-2008-000-20253-0) to I.S. Jang. In addition, this work was supported by the Technology Development Program for Agriculture and Forestry (108069-03-1-CG000), Ministry for Agriculture, Forestry and Fisheries.
All Science Journal Classification (ASJC) codes
- Complementary and alternative medicine