Mouse genetics: Catalogue and scissors

Young Hoon Sung, In Jeoung Baek, Je Kyung Seong, Jin Soo Kim, Han Woong Lee

Research output: Contribution to journalShort survey

22 Citations (Scopus)

Abstract

Phenotypic analysis of gene-specific knockout (KO) mice has revolutionized our understanding of in vivo gene functions. Asthe use of mouse embryonic stem (ES) cells is inevitable for conventional gene targeting, the generation of knockout mice remains a very time-consuming and expensive process. To accelerate the large-scale production and phenotype analyses of KO mice, international efforts have organized global consortia such as the International Knockout Mouse Consortium (IKMC) and InternationalMouse Phenotype Consortium (IMPC), and they are persistently expanding the KO mouse catalogue that is publicly available for the researches studying specific genes of interests in vivo. However, new technologies, adopting zinc-finger nucleases (ZFNs) orTranscription Activator-Like Effector (TALE) Nucleases (TALENs) to edit the mouse genome, are now emerging as valuable and effective shortcuts alternative for the conventional gene targeting using ES cells. Here, we introduce the recent achievement of IKMC, and evaluate the significance of ZFN/TALEN technology in mouse genetics.

Original languageEnglish
Pages (from-to)686-692
Number of pages7
JournalBMB reports
Volume45
Issue number12
DOIs
Publication statusPublished - 2012 Dec 1

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Sung, Y. H., Baek, I. J., Seong, J. K., Kim, J. S., & Lee, H. W. (2012). Mouse genetics: Catalogue and scissors. BMB reports, 45(12), 686-692. https://doi.org/10.5483/BMBRep.2012.45.12.242