Abstract
Phenotypic analysis of gene-specific knockout (KO) mice has revolutionized our understanding of in vivo gene functions. Asthe use of mouse embryonic stem (ES) cells is inevitable for conventional gene targeting, the generation of knockout mice remains a very time-consuming and expensive process. To accelerate the large-scale production and phenotype analyses of KO mice, international efforts have organized global consortia such as the International Knockout Mouse Consortium (IKMC) and InternationalMouse Phenotype Consortium (IMPC), and they are persistently expanding the KO mouse catalogue that is publicly available for the researches studying specific genes of interests in vivo. However, new technologies, adopting zinc-finger nucleases (ZFNs) orTranscription Activator-Like Effector (TALE) Nucleases (TALENs) to edit the mouse genome, are now emerging as valuable and effective shortcuts alternative for the conventional gene targeting using ES cells. Here, we introduce the recent achievement of IKMC, and evaluate the significance of ZFN/TALEN technology in mouse genetics.
| Original language | English |
|---|---|
| Pages (from-to) | 686-692 |
| Number of pages | 7 |
| Journal | BMB reports |
| Volume | 45 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2012 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
Cite this
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Mouse genetics : Catalogue and scissors. / Sung, Young Hoon; Baek, In Jeoung; Seong, Je Kyung; Kim, Jin Soo; Lee, Han Woong.
In: BMB reports, Vol. 45, No. 12, 01.12.2012, p. 686-692.Research output: Contribution to journal › Short survey
TY - JOUR
T1 - Mouse genetics
T2 - Catalogue and scissors
AU - Sung, Young Hoon
AU - Baek, In Jeoung
AU - Seong, Je Kyung
AU - Kim, Jin Soo
AU - Lee, Han Woong
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Phenotypic analysis of gene-specific knockout (KO) mice has revolutionized our understanding of in vivo gene functions. Asthe use of mouse embryonic stem (ES) cells is inevitable for conventional gene targeting, the generation of knockout mice remains a very time-consuming and expensive process. To accelerate the large-scale production and phenotype analyses of KO mice, international efforts have organized global consortia such as the International Knockout Mouse Consortium (IKMC) and InternationalMouse Phenotype Consortium (IMPC), and they are persistently expanding the KO mouse catalogue that is publicly available for the researches studying specific genes of interests in vivo. However, new technologies, adopting zinc-finger nucleases (ZFNs) orTranscription Activator-Like Effector (TALE) Nucleases (TALENs) to edit the mouse genome, are now emerging as valuable and effective shortcuts alternative for the conventional gene targeting using ES cells. Here, we introduce the recent achievement of IKMC, and evaluate the significance of ZFN/TALEN technology in mouse genetics.
AB - Phenotypic analysis of gene-specific knockout (KO) mice has revolutionized our understanding of in vivo gene functions. Asthe use of mouse embryonic stem (ES) cells is inevitable for conventional gene targeting, the generation of knockout mice remains a very time-consuming and expensive process. To accelerate the large-scale production and phenotype analyses of KO mice, international efforts have organized global consortia such as the International Knockout Mouse Consortium (IKMC) and InternationalMouse Phenotype Consortium (IMPC), and they are persistently expanding the KO mouse catalogue that is publicly available for the researches studying specific genes of interests in vivo. However, new technologies, adopting zinc-finger nucleases (ZFNs) orTranscription Activator-Like Effector (TALE) Nucleases (TALENs) to edit the mouse genome, are now emerging as valuable and effective shortcuts alternative for the conventional gene targeting using ES cells. Here, we introduce the recent achievement of IKMC, and evaluate the significance of ZFN/TALEN technology in mouse genetics.
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UR - http://www.scopus.com/inward/citedby.url?scp=84872348123&partnerID=8YFLogxK
U2 - 10.5483/BMBRep.2012.45.12.242
DO - 10.5483/BMBRep.2012.45.12.242
M3 - Short survey
VL - 45
SP - 686
EP - 692
JO - BMB Reports
JF - BMB Reports
SN - 1976-6696
IS - 12
ER -