MRF family genes are involved in translation control, especially under energy-deficient conditions, and their expression and functions are modulated by the TOR signaling pathway

Du Hwa Lee, Seung Jun Park, Chang Sook Ahn, Hyunsook Pai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Dynamic control of protein translation in response to the environment is essential for the survival of plant cells. Target of rapamycin (TOR) coordinates protein synthesis with cellular energy/nutrient availability through transcriptional modulation and phosphorylation of the translation machinery. However, mechanisms of TOR-mediated translation control are poorly understood in plants. Here, we report that Arabidopsis thaliana MRF (MA3 DOMAIN-CONTAINING TRANSLATION REGULATORY FACTOR) family genes encode translation regulatory factors under TOR control, and their functions are particularly important in energy-deficient conditions. Four MRF family genes (MRF1-MRF4) are transcriptionally induced by dark and starvation (DS). Silencing of multiple MRFs increases susceptibility to DS and treatment with a TOR inhibitor, while MRF1 overexpression decreases susceptibility. MRF proteins interact with eIF4A and cofractionate with ribosomes. MRF silencing decreases translation activity, while MRF1 overexpression increases it, accompanied by altered ribosome patterns, particularly in DS. Furthermore, MRF deficiency in DS causes altered distribution of mRNAs in sucrose gradient fractions and accelerates rRNA degradation. MRF1 is phosphorylated in vivo and phosphorylated by S6 kinases in vitro. MRF expression and MRF1 ribosome association and phosphorylation are modulated by cellular energy status and TOR activity. We discuss possible mechanisms of the function of MRF family proteins under normal and energy-deficient conditions and their functional link with the TOR pathway.

Original languageEnglish
Pages (from-to)2895-2920
Number of pages26
JournalPlant Cell
Volume29
Issue number11
DOIs
Publication statusPublished - 2017 Nov 1

Fingerprint

Sirolimus
translation (genetics)
Starvation
Ribosomes
starvation
energy
ribosomes
Genes
genes
TOR Serine-Threonine Kinases
Phosphorylation
phosphorylation
Ribosomal Protein S6 Kinases
Plant Cells
Protein Biosynthesis
Arabidopsis
proteins
Sucrose
Proteins
nutrient availability

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

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title = "MRF family genes are involved in translation control, especially under energy-deficient conditions, and their expression and functions are modulated by the TOR signaling pathway",
abstract = "Dynamic control of protein translation in response to the environment is essential for the survival of plant cells. Target of rapamycin (TOR) coordinates protein synthesis with cellular energy/nutrient availability through transcriptional modulation and phosphorylation of the translation machinery. However, mechanisms of TOR-mediated translation control are poorly understood in plants. Here, we report that Arabidopsis thaliana MRF (MA3 DOMAIN-CONTAINING TRANSLATION REGULATORY FACTOR) family genes encode translation regulatory factors under TOR control, and their functions are particularly important in energy-deficient conditions. Four MRF family genes (MRF1-MRF4) are transcriptionally induced by dark and starvation (DS). Silencing of multiple MRFs increases susceptibility to DS and treatment with a TOR inhibitor, while MRF1 overexpression decreases susceptibility. MRF proteins interact with eIF4A and cofractionate with ribosomes. MRF silencing decreases translation activity, while MRF1 overexpression increases it, accompanied by altered ribosome patterns, particularly in DS. Furthermore, MRF deficiency in DS causes altered distribution of mRNAs in sucrose gradient fractions and accelerates rRNA degradation. MRF1 is phosphorylated in vivo and phosphorylated by S6 kinases in vitro. MRF expression and MRF1 ribosome association and phosphorylation are modulated by cellular energy status and TOR activity. We discuss possible mechanisms of the function of MRF family proteins under normal and energy-deficient conditions and their functional link with the TOR pathway.",
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MRF family genes are involved in translation control, especially under energy-deficient conditions, and their expression and functions are modulated by the TOR signaling pathway. / Lee, Du Hwa; Park, Seung Jun; Ahn, Chang Sook; Pai, Hyunsook.

In: Plant Cell, Vol. 29, No. 11, 01.11.2017, p. 2895-2920.

Research output: Contribution to journalArticle

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